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Convenient Method for the Determination of Foaming Properties of Egg White and Its Verification

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(1)KOREAN J. FOOD SCI. TECHNOL. Vol. 36, No. 5, pp. 728~732 (2004). ©The Korean Society of Food Science and Technology. ÂW –®;9ËK 5 n;9 ï&¢ *‚ *Þ /;O» Ï9 ¦Ã B¢Áªæ* ]L ®'·. Convenient Method for the Determination of Foaming Properties of Egg White and Its Verification Mi-Ra Kim and Jee-Young Imm* Department of Food and Nutrition, Kookmin University Convenient method for determination of egg white foaming properties was suggested. Highly reproducible results were obtained when 70 g egg white was whipped at 1,200 rpm for 2 min. Using standardized testing method, foaming capacity and stability of egg white samples including fresh egg white, freeze-dried egg white and commercial egg white powder were effectively differentiated. Foaming capacity of egg white was not affected during storage of fresh egg white up to 4 weeks at 4oC, whereas foaming stability significantly decreased after 2 weeks of storage. Key words: foaming property, method, standardization, egg white. *. †. ÂWªö~ –®;WËKj ËçÊV *šBº ¢N'b ‚ –®;WËK~ ;{‚ ï&O»š jº~ . *Òræ  W~ –®;WËKj ï&~V *‚ ’º æ³'b‚ ê¯ >Ú zb¾ ‚&zB O» 5 G;Vêº jçræ BB>Ú ®æ pb– shaking, bubbling/sparging, whipping ~ ·‚ öÒ¢ šÏ‚ G;O»š  >î (4-6). ¾ &¦ª~ O» f G;š Ç~–¾ G;"; 7 –.>æ pf æ> ‚ ž~ ç^jv& ®&Ë~– &~ Ëj¢ jº‚ ~V ê ‚ . ÂW –®;WËK~ ï&O» 7 &Ë Þ'b‚ ÒÏ> ®º O»f whippingb‚Ž B" B› ~ B  ®Ö ;" ¸f FÒWj &æ ®b¾ '‚ –®;W –šš¾ G;O»~ Ò*Wö &‚ ’º B : ìb– ’¶ö V¢ ª~'b‚ æ;>–¾ ôf ¦ªj ãþö ~ š~ ®º ;š .  ’º ÂW –®;WËK 5 n;W~ ;{‚ ï&¢ * ~ –® ;W  R«B ò~ ·, whipping * 5 ³ê j –.~ ‚&zB G; O»j B~¶ ~&b– G ;O»~ Ò*Wj ¦Ã~ ÖëÚöB *Þ~² 'Ï>º © j Ï'b‚ ~& .. ®Wî~ bÒz' VËWf ®~ ®î" V^Wö ç7'ž 'Ëj ~º ºžb‚B ’² Ϛê, >K  Wî" >ª~ ç^·Ïö ~š Ö;>º Wî, FzËK 5 –®;WËK" ?š ‡Ú-‡Ú, ‡Ú-VÚ~ êšöB ·Ï~ º Wî, º;W" ?f Wî *~ ç^·Ïö ~š Ö;> º Wî‚ ’ª† > ® (1). šf ?f Wî~ VË' ß Wf Wî~ «~, ’V, ²>W, jžÖ V, ’– " ?f Wî~ ÚÒ' º²f Nê, pH, "~ ;ê  Wî š 7~² >º ~ã ºžö V¢ š² ‚*B (2). ÂWf –®;W, º;W 5 Ú ÖOËK ~ ·‚ VËWj F~ ®º Wî ²Ò‚Ž B", B› 5 GB ® ~ &®ö n®® ÒÏ> ®b– ß® –®;WË Kf ›ö ¦bf ¦æj B~ ËV Wª~ ªÖö ê æj & (3). ‚"~ &®f ÞҎj º’~º “ready to eat type”~ ޚ' VË~ 7ºWš Ã&~º º^š– š‚ ;~ B® B–¢ *šBº /" *' GšöB ‡ç  W Ë6j &ê ªö ;~ ®²Ò& F^F > ®b – ÂWªö~ šz' VËWš ‡çÂW ËçF ãÖ  ÎÏWf z× Ã&† > ® .. Òò 5 O» Òò ÂWf –ž(")öB /‚ F‚ fj †¦~ j B–‚ ê ÒÏ~&b– ÿ¢‚ ÂWj ÿ֚–~ ÿ֚ –ÂWj B–~& . ªZš–‚ B–B çëÏÂWªöf Ovonor(France)Ò~ B®j ÒÏ~& .. *Corresponding author: Jee-Young Imm, Department of Food and Nutrition, Kookmin University, 861-1 Chongnung-dong, Songbukgu, Seoul 136-702, Korea Tel: 82-2-910-4772 Fax: 82-2-911-4771 E-mail: jyimm@kookmin.ac.kr 728.

(2) ÂW –®;WËK 5 n;W ï&¢ *‚ *Þ G;O»" ÏW ¦Ã. –®;9ËK~ ï& –®G;Ë~º ò~ &“ö Vž v>³ê~ æz&  ;F > ®º electronic heavy duty stirrer(IKA, Nara, Japan)¢ ÒÏ~&b– "OÏ –®Vö ÒÏ>º ©" ÿ¢‚ *‚Æ ¢ ËO~ ò~ ·(60-80 g), v>*(1-3ª), v>³ê (1,000-1,400 rpm)¢ æzʖ –®;W~ ‚'–šj J;~ & . –®;W~ G; öº Fig. 1(a)öB BB :f ?š ²*»" –®;WÏV~ 㚠–Ò 5 *‚Æ~ ‚~" : š~ –Ò¢ ¢;~² Fæ~ *‚Æ¢ ²*  B~ º öKö ~‚ –®;W &š¢ B–~& . ;WB –®~ ¦bº –®;WÏV ãšö ‚B ..F(100-1,000 mL, 10 mL *Ï)j £Ú G;~&b– overrun(%)b‚ ¾æÚî . Overrun(%) òχ 100 mL~Z² – –® 100 mL~Z² =  ------------------------------------------------------------------------------------------------------------------------------ × 100 –® 100 mL~Z² –®n;9 ï& –®;WÏV~ : šf Fig. 1(b)f ?š ¢;‚ ’V(2 mm) f *Ï~ ’sj ÚÚ *~ ã"ö Vž –®n;Wj ï &~& . –®;W  ÏV~ : š" ÿ¢‚ ’V~ Ò~ N¢ ÏV : ö ËO~ –®;W 7 ò~ FÂj j ã® N~& . –® ¦b~ G; ê ÏV : š~ Ò~ N¢ B–~ drainage testing unit(Fig. 1(c))b‚ vÚ~&b – ¢;* *Ïb‚ ‡zB –®~ Z²¢ digital balance(Ohaus, USA)¢ šÏ~ G;~& . –®WËK" n ;W~ ï& öº ïË&Ë 7ž fj çNö 1* O~. Fig. 1. Schematic presentation of foaming testing unit. a: foaming apparatus, b: bottom of container, c: drainage testing unit.. 729. ~ ò~ Nê Nšö ~‚ –®WËK 5 n;W~ æ z¢ ‚²z~& . 6‚ ÿ¢‚ –šöB 20² > G;~ ï&O»~ Ò*Wj ¦Ã~& . ‚&z* O»~ Ï9 ¦Ã ‚&zB O»~ ÏWj ¦Ã~V *~ F‚ ÂW, ÿ ֚–ÂW, ªZš–ÂWª~ –®;WËK" n;W~ Nš ¢ –Ò~&b–, F¦j 4oCöB 4"* &Ë~– &ËV* ö Vž –®;WËK" n;W~ æz¢ G;~& . Ûêª+ Î þf 3² šç > G;~&b–, Ö"~ ÛêªC f Minitab Ver. 13.1(Minitab inc., USA)j šÏ~& . ¾Ò’ *~ F~' Nš(p < 0.05)º ANOVAf Tukey~ 7jv¦; »j ÒÏ~& .. Ö 5 8 –®;9ËK~ ï& .V òï~ æz& –® overrunö ~º Î"¢ –Ò~ V *~ 1,000 rpm, 2ªb‚ whipping –šj ;~ F ‚ ÂW~ –® overrunj G;~& . Fig. 2ö ¾æ :f ? š 70 g~ ò¢ Òς ãÖ 60 6º 80 g~ òf jv† r F~'b‚ ¸f overrunj ¾æÚî . –®;W~ G;ö ÒÏ>º '.‚ ò~ ·f G;ÏV 5 *‚Æ¢~ dimension ö V¢ æzF > ®b¾  þ–š~ ãÖ 60 g š~~  ò¢ R«‚ ãÖ *‚Æ¢f ÂW"~ 7/šš 6²~ Ï ª‚ whippingš ¢Ú¾æ p~b– 80 g šç~ ò¢ ÒÏ ‚ ãÖ –®š *‚Æ¢~ ‚çræ ;W>Ú z šç~ – ®š W>æ p~ . ‚Þ 60-80 g~ ò º*öBº R« B ò~ Îv& –®;Wö ÒÏ>îb– whipping ê ‡Ú ç‚ šÒ~º òº &V>æ p~ . ÂW –®;W";~ ª¶' V*f jç «{® rJ^ ® æ pb– æ ÂWö šÒ~º Wî~ ç^·Ï~ Ö"‚ B Ö bç'b‚ šš> ® (7). Johnson" Jabik(8)f. Fig. 2. Foam overrun of fresh egg white determined at various initial sample weights. a-b : Means with different superscript are significantly different (p<0.05)..

(3) ‚“®"²æ B 36 ² B 5 ^ (2004). 730. Table 1. Overrun of fresh egg white determined at various whipping conditions Whipping speed (rpm) 1,000 1,200 1,400. Overrun (%) at whipping time (min) 1. 2. 3. 430Û15.5b,y 524Û12.9a,y 311Û18.7c,y. 568Û0b,x0.0 623Û15.3a,x 638Û4.4a,x0. 574Û11.4a,x 628Û12.8a,x 643Û25.7a,x. a-c. : Means with different superscript within a column are significantly different (p<0.05). x-y : Means with different superscript within a row are significantly different (p<0.05).. angel cake systemöB –®~ ;Wö Vê& ¸f Wîf globulins, ovalbumin, ovotransferrin, lysozyme, ovomucoid, ovomucin~ Bšî  ~& . –®;Wj *‚ ‚' òï(70 g)j Ö;‚ ê whipping ³ ê 5 *ö Vž –®;WËKj G;~& . Table 1" ? š ;WB –®~ overrunf whipping ³ê(1,000-1,400 rpm) 5 *~ Ã&(1-3ª)ö V¢ Ã&~º ãËj ¾æîb¾ 1,200 rpm, 2ª šç~ –šöBº whipping ³ê 5 *~ Ã&ö V¢ WB –® ¦b~ F~'ž Ã&º ¾æ¾æ p~ . ‚Þ, Table 1~ G; –š z †ž whipping ³ê 5  *~ Ã& öº –®~ Žö *çš &V>î . š‚  W~ overbeating *çf ÂWö šÒ~º ovalbumin ª¶~ æ Wš Ã&~– VÚf ‡Ú~ êšöB w÷>Ú vâÚ lamellae [j ;W~V r^ž ©b‚  >Ú ® (9). Whippingš æ³Nö V¢ ;W>º ovalbumin ª¶~ w÷f ‚š‚Wj 6²ʖ ®ÏW~ «¶‚ *~>Ú –®~ »Z& ¢Ú¾ º ©b‚ 6>– –®¾~ //~ F‚ ž&j ç. ~&bæ‚ z šç~ whipping *~ Ã&º J~æ p~ . –®;9ËK 5 n;9~ Ò*9 ï& ï& O»~ Ò*Wj ¦Ã~V *~ *~ þöB J; B whipping –š(òï 70 g, whipping ³ê 1,200 rpm, whipping * 2ª)öB F‚ ÂW~ –®;WËKj 20² > G;~& (Fig. 3(a)). G;B –®~ ï overrunf 622.8%, ‚&ÞNº 29.4‚B Ö ¸f Ò*Wj ¾æÚîb– –®¦ b~ G;  ¾æ JN~ ¢¦º G;"; 7 ò~ O~ * Nš 5 ò~ ®¢Wö Vž ©b‚ 6B . V¢ B –®;W * ò~ Nê 5 çNO~ *j –.† ãÖ  ;{‚ –®;W ËK~ ï&& &ˆ ©b‚ 'B . 6‚ –®~ Wïj .Vò~ ¦bö &‚ Ã&ïb‚ ‚ *‚ ãÖöê ÿ¢‚ Ö"& &V>îb– V¢B Öë' w Ïj *šBº B® WB –®~ ¦b¢ G;~ ò~ –®;WËKj jv~ê ^B& ìj ©b‚ 6B . ;WB –®~ n;Wf ¢>'b‚ –®~ W ê *š ã"Žö V¢ –®zB ‡š  ‡z>º ïj G;~º ö Ò¢ ÒÏ~ ® . VÂB ‡Úïf 7ï, ¦b _f .V ò~ 7ï(¦b)ö &‚ jN(%)‚ ‚*† > ®b– Sathef Salunkhe(10)f –®~ ;W ê ¢;ï~ –®j ;ï'b‚ : ö ’sj &ê ÏV‚ TÎ –®~ n;Wj G;~& . ¾ –®~ ;ï' šÿf * 'b‚ £æ pb– ôf G ; JN¢ ¢bÒ > ® .  þöBº –®~ ;W  ’ sš ÑÖ ÏV~ : šj ÿ¢‚ ’V~ silicon îB‚ ”Ú. Fig. 3. Frequency distribution of foam overrun (a) and drainage weight (b) of fresh egg white during 20 times repeated measurements.. ò~ ¶ ìš –®j ;W~&b– –®~ ;W ê îB ¢ B–~ Fig. 1(c)f ?f drainage test unitb‚ TÎ ³ ‚ –®n;W G;š &Ë~êƒ ~& .  ’öBº –®n;W ï&~ Î ãÖ –®;Wçê ¦V 90ªræ 15ª *Ïb‚ drainage weight~ æz¢ Îv G;~&b¾ Ö"~ ‚* öº 45ª ê G;‚ drainage weight‚ ¾æÚî . Fig. 3(b)ö ¾æ :f ?š £ 45ª~ ã" öº ‡zB –®~ ïZ²º 8.6 g ‚&ÞN 2.7j ¾æÚÚ > G;  ¸f Ò*Wj ¾æÚîb– &¦ª~ òöB ÒÏB òZ²~ £ 20% šçš ‡z>º ©b‚ ¾æÒ . –®n;W~ G;O»ö ®ÚB Zhuf Damodaran(11)f ö êB column ʂ ÚöB –®~ »Z‚ ¢V>º {K~ à &¢ G;~ –®n;W~ æz¢ B~& . Davisf Foegeding(12)f  þöBf ?š foam lamellae¢ Û~ ’s  ÏV~ : šb‚ FÂ>º ò~ ·j G;~&b– ‡ zB ò& *ږ®Z²~ 50%& >º– ²º>º *b ‚ ‚*~& .  ’öB B‚ ‚* O»f –®~ n; W" B &;  ²º>º *j J~ ß;* Ú ~ n;W 悂 ‚φ > ®j ©b‚ '>– 30ª šç ~ ã" öº Îv ¸f Ò*Wš &V>î (Ö" B)..

(4) ÂW –®;WËK 5 n;W ï&¢ *‚ *Þ G;O»" ÏW ¦Ã. ·‚ ÂW ò~ –®;9ËK 5 n;9 æz /; „B B‚ –®;WËK 5 n;W ï&O»~ FÏWj –Ò~V *~ F‚ ÂW, F‚ ÂWj ÿ֚–~ B –‚ ÿ֚– ÂWªö 5 çëÏ ÂWªö~ –®;WËK " n;Wj G;~& (Fig. 4). F‚ ÂW" ÿ¢‚ ;ª Žïj &æêƒ ªöj Ã~>ö ϚB G;‚ ò~ – ®;W ËKf F‚ ÂW, ÿ֚–ÂW, ªZš– ÂW~ B b‚ ¸f ©b‚ ¾æÒb– ;WB –®~ overrunj V&b ‚ ~&j r ÿ֚– ªö~ –®;W ËKf F ÂW~ 80%, ªZš– ÂWf 40% >&b‚ F~'b‚ 6²~&. (P < 0.05). –®n;W~ ãÖöê F‚ ÂWš ¢;* Ú ‡zB ·š &Ë ·j F~'b‚ ¸f n;Wj ¾æîb– ÿ֚–ÂW" ªZš– ÂW *öê F~'ž Nš& &V >î (P < 0.05). š Ö"º  ’öB Òς ï&O»j š Ï~ ' ò~ –®;W ËK 5 n;W~ Nš¢ Î"' b‚ ’êš â > ®rj ~~– š–"; 7 –®;Wö 7º‚ †j >¯~º Wî~ æz& ¢Ú¾º ©b‚  '† > ® . š– –šš ÖFWî~ VË ßWö ~º Î"ö &‚ ’ " jv† r fWî~ VËßW æzö &‚ ’. Fig. 4. Foam overrun (a) and drainage weight (b) of fresh egg white (FEW), freeze-dried egg white (FDEW) and commercial spary-dried egg white powder (SDEW). a-c : Means with different superscript are significantly different (p<0.05).. 731. º Ö B‚'b‚ šÚr . Franke and Kie ling(13)º š –Nê¾ ò~ ªZ  &šæº {Kö V¢ ªZš–*¦ ªö~ VËßWš ¢î > ®rj ~&b¾ æzB V ËßWj J«† > ®º ÂWWî ~ ’–' æWš¾ ² >W, SH Žï ~ æzº –Ò~æ p~ . *Ò  ’& öBº š–";öB ¢Ú¾º Wî~ æz& –®;WËK 5 n;Wö ~º 'Ëj –Ò~ ® . ê¦~ &Ë8*ö 8ž –®;9ËK 5 n;9æz F‚ ê¦j 4"ræ 4oCöB &Ë~– &ËV*ö Vž –®;WËK 5 n;W~ æz¢ G;~& . ÂW~ –®; WËKf –ÒB &ËV* ÿn F~'ž Nš& ¾æ¾æ p f >š –®n;W~ ãÖ &Ë 2" ã" ê¦V F~'b‚ 6²~& (p < 0.05). šf ?f Ö"º ïË &Ë  &ËV* ~ Ã&ö V¢ foam overrunöº Nš¢ šæ p~b¾ n ;Wf F~'b‚ 6²~& º Vš~ f ¢~~&. (14). ÂW~ –®;WËK 5 n;Wf ÂWj ’W~º  Wî~ *~ßW" &7‚ &ê& ®b– ß® *6š 10.7 ž lysozyme" ;ç'ž f~ pH(9.0)öB rb‚ ~*B. ž Wî"~ ç^·Ïš Ö 7º‚ †j ~º ©b‚ r J^ ® (15). –®;W  êšöB ¢Ú¾º lysozyme". Fig. 5. Changes in foam overrun (a) and drainage weight (b) of egg white during storage at 4oC. a-b : Means with different superscript are significantly different (p<0.05)..

(5) ‚“®"²æ B 36 ² B 5 ^ (2004). 732. ž ÂWWî"~ ;*V'֏f ;*V' >BK~ 6²¢ ¢bÚb‚Ž –®~ n;Wj ËçÒ > ®rš  >î b–(16,17) &ËV* 7 ¢Ú¾º pH çßf &ËV*~ Ã& ö V¢ ¾æ¾º –®n;W~ 6²f &ê& ®j ©b‚  'B .. º. £.  ’öBº ÂW~ –®;WËK 5 n;Wj *Þ~² ï&~V *‚ ‚&O»j B~¶ ò~ ·, whipping ³ ê 5 *š –®;Wö ~º 'Ëj –Ò~ '‚ – ®;W–šj F;~& . F;B –š(òï 70 g, 1,200 rpm, 2ª)b‚ ÂW~ –®;WËK 5 n;Wj 20² > G;~  ï&‚ Ö" ¸f Ò*Wj ¾æÚîb– ò~ æz(F ‚ ÂW, ÿ֚– ÂWªö, çëÏ ÂWªö) 5 ê¦~ &Ë V*ö Vž –®;WËK 5 n;W~ F~' Nš¢ Î"' b‚ ’ê† > ®î .. 6Ò~   ’º 2003jê ³âVFBBÒë~ ’j æöb‚ > ¯>îb– šö 6Òãî . 6‚ ’j~ ¢¦f ò¢ Bš & –ž"²Òö² 6Òãî .. ^. ò. 1. Kinsella JE. Functional properties of food proteins: A review. CRC Crit. Rev. Food Sci. Nutr. 7: 219-280 (1976) 2. Damodaran S. Functional properties. pp. 167-234. In: Food Proteins. Nakai S, Modler HW (eds). VCH Publishers Inc., New York, NY, USA (1996) 3. Kinsella JE. Functional properties of protein: Possible relationships between structure and function in foams. Food Chem. 7: 273-288 (1981) 4. Halling PJ. Protein-stabilized foams and emulsions. CRC Crit.. Rev. Food Sci. Nutr. 15: 155-203 (1981) 5. Baniel A, Fains A, Popineau Y. Foaming properties of egg albumen with a bubbling apparatus compared with whipping. J. Food Sci. 62: 377-381 (1997) 6. Hagolle N, Relkin P, Popineau Y, Bertrand D. Study of the stability of egg white protein-based foams: effect of heating protein solution. J. Sci. Food Agric. 80: 1245-1252 (2000) 7. Mine Y. Recent advances in the understanding of egg white protein functionality. Trends Food Sci. Technol. 6: 225-232 (1995) 8. Johnson TM, Zabik ME. Response surface methodology for analysis of protein interactions in angel cakes. J. Food Sci. 46: 12261230 (1981) 9. Lau K, Dickinson E. Structural and rheological properties of aerated high sugar systems containing egg albumin. J. Food Sci. 69: 232-239 (2004) 10. Stahe SK, Deshpande SS, Salunkhe DK. Functional properties of lupin seed proteins and protein concentrates. J. Food Sci. 47: 491-497 (1982) 11. Zhu HM, Damodaran S. Heat induced conformational changes in whey protein isolate and its relation to foaming properties. J. Agric. Food Chem. 42: 846-855 (1994) 12. Davis JP, Foegeding EA. Foaming and interfacial properties of polymerized whey protein isolate. J. Food Sci. 69: 404-410 (2004) 13. Franke K, Kieβling M. Influence of spray drying conditions on functionality of dried whole egg. J. Sci. Food Agric. 82: 18371841 (2002) 14. Hammershøj M, Qvist KB. Importance of hen age and egg storage time for egg albumen foaming. Lebensm. Wiss. Technol. 34: 118-120 (2001) 15. Poole S, West SI, Walters CL. Protein-protein interactions: The importance in the foaming of heterogeneous protein systems. J. Sci. Food Agric. 35: 701-711 (1984) 16. Clark DC, Mackie AR, Smith LJ, Wilson D. The interaction of bovine serum albumin and lysozyme and its effect on foam composition. Food Hydrocol. 2: 209-223 (1988) 17. Damodaran S, Anand K, Razumovsky L. Competitive adsorption of egg white proteins at the air-water interface: Direct evidence for electrostatic complex formation between lysozyme and other egg proteins at the interface. J. Agric Food Chem. 46: 872-876 (1998) (2004j 9ú 3¢ %>; 2004j 10ú 12¢ j).

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