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Fast Protein Liquid
Chromatography (FPLC )
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Column components
1. Packing
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Gel degassing.
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Stopping the outlet of the column.
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Filling liquid in the bottom of the column
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Pouring gell slurry to the column.
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Settling of gels.
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Adjusting the adaptor
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Fixed adaptor
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Conneting a line to the adaptor
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Adaptor touching
the upper layer of gels.
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Gel level lowers due to the solution flow
into the column.
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Stopping the outlet
line
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Connetion of pump
line to the adaptor
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Blue dextran solution flows in the column.
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2. Valve control
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Injection of sample
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FPLC controller.
3. Remote controller
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Pump front panel.
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Pump flow rate calibration
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Manual mode of gradient setting
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Valve number setting
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Flow rate setting
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Multimeter for digital data aquisition
4. Multimeter
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UV detector
5. Detector
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Software UV signal data aquisition
6. Software(PROTEK 506)
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Multimeter setting for sending data
to notebook computer
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Sampling time setup
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File name.
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Save data file In a folder
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Starting data acquisition End ing data collection
