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￭ Sat-021 ￭
Microbial signature associated with prognosis of Crohn’s disease
1강북삼성병원 내과, 2강북삼성병원 소화기내과, 피디젠, 3유전체 연구소
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김유경
1, 박수경
2, 양효준
2, 박동일
2, 조성훈
3, 안광성
3Background/Aims: Limited studies have examined the intestinal microbiota composition in relation to prognosis of inflammatory bowel disease (IBD). We aim to analyze the alteration of the intestinal microbial community structure in Crohn’s disease (CD) patients according to the prognosis. Methods: 16S rRNA genes from fecal samples of 32 CD patients and 58 healthy controls (HC) were amplified using a universal primer set and sequenced with Illumina Mi-Seq. CD patients who used 2 or more biologics or undergone bowel resection (poor prognosis group, n=17) and who used only 5-ASA or im- munomodulators (good prognosis group, n=15) were included in this study. The microbial composition and diversity of each sample were analyzed with the QIIME, and the association between prognosis was investigated. Results: The contribution of bacterial groups to the intestinal microbial composition differed between CD and HC and between CD with good and poor prognosis. On taxonomic comparison at a genus level, relative abundances of Fusobacterium and Sutterella were increased CD patients with poor prognosis than good prognosis group or HC. The relative abundances of Ruminococcus were similar between HC and CD good prognosis group, but higher than poor prognosis group. The relative abundances of Lachnospira and Clostridium were higher in HC than both good and poor prognosis group of CD patients [all false discovery rate (FDR), P<0.05]. Conclusions: Our 16S rRNA sequence data demonstrate differ- ent intestinal dysbiosis in CD patients compared to HC and according to prognosis of CD. Specific microbiome might be used as microbiomarkers to predict prognosis of CD.
￭ Sat-022 ￭
What is benefit to evaluate the endoscopic severity of UC patients way to use sigmoidscopy
조선대학교병원
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이승우
Background and Aim: Ulcerative colitis(UC) is a chronic inflammatory bowel disease of unknown etiology and is a lifelong disease repeating clinical improvement and aggravation. Endoscopic severity assessment is very important, because the severity assessment is related to the prognosis of the UC patient. Colonoscopy is able to identify the entire field, but is accompanied by complication and the preparation process is difficult. The aim of this study is to evaluate the endoscopic tool to assessing severity of ulcerative colitis during follow up UC patients. Method: The subjects were 183 UC patients who were diagnosed and followed up at Chosun University Hospital from 2013 January to 2017 December. Among them, 101 patients with follow-up colono- scopy were enrolled and retrospectively evaluated for endoscopic severity. The severity is assessed by colonoscopy alone, inspector determines endoscopic severity of follow up UC patients from rectosigmoid and proximal colon. The scale of endoscopic severity is endoscopic mayo score and ulcerative colitis endoscopic index scale(UCEIS). Result: Of the 101 patients, 40 has lesions limited to the rectosigmoid colon.(39%) The average of endoscopic mayo score of entire colon is 1.21 and in case of rectosigmoid colon is 1.07. The average of UCEIS of entire colon is 2.24 and in case of rectosigmoid colon is 1.94.
The agreement endoscopic mayo score between the each site is observed with a kappa value of 0.83(p=0.00), and agreement of UCEIS between the each site is observed with a kappa value of 0.840 (p=0.00) Conclusion: There is a very high level of agreement between entire colon severity and rectosigmoid colon severity in followed UC patients. Sigmoidoscopy is a good modality for evaluating the endoscopic severity of followed UC patients, considering complication and high cost.
