인삼으로부터
Acyl-CoA-binding Protein
유전자의 동정 및 계통적 분석인준교・류명현・최광태・최관삼1・김세영2・양덕춘*
한국인삼연초연구원 신사업연구부,1충남대학교 응용생물화학부,2경희대학교 농학과
Isolation and Phylogenetic Analysis of Acyl-CoA-binding Protein Gene from Panax ginseng C.A. Meyer
IN, Jun Gyo・ RYU, Myong Hyun・ CHOI, Kwang Tae・CHOI, Kwan Sam
1・KIM, Se Young
2・YANG, Deok Chun*
Korea Ginseng & Tobacco Research Institute, Taejon, 305-345, Korea
1
Department of Applied Biology, Chungnam National University, Taejon, 305-764, Korea
2
Department of Agronomy, Kyung Hee University, Suwon, 449-701, Korea
A B S T R A C T Acyl-CoA b ind ing protein s (ACBP) are small highly-conserved cytosolic p ro teins that bind lon g-chain acyl-CoAs. A c DNA en cod ing ACBP was iden tified fro m cDNA libra ry co ns tructed fro m hairy ro ot p oly A+ RNA in expressed seq uen ce tag s (EST) analysis. The cDNA clo ne was 4 53 bp lo ng and carried an o pen read ing frame of 2 6 4 bp (1 0 k Da). Th e ginseng ACBP amin o acid seq uen ce was co mp ared with o th er repo rted plant ACBPs us in g th e CLUSTALW. Gins eng ACBP is 8 9%, 81 %, 8 0 %, an d 7 3% id entica l with ACBP from casto r bean , lilly, D i g i t a l i s a nd A r a b i d o p s i s, resp ectively . Ho wever, g inseng ACBP is 5 amin o acids smaller than A r a b i d o p s i s an d rap e se ed ACBPs. Als o there is n o an y kno wn sign al pep tide s equ ence in g inseng ACBP.
Key wo rd : Acyl-CoA-bind ing p ro tein (ACBP), EST, h airy roo t, Panax gins eng
*Corresponding author. Tel 042-866-5434 Fax 042-862-2522 E-mail [email protected]
식물조직배양학회지 제28권 제4호
