Journal of Sasang Constitutional Medicine

Abstract

실험재료 1.

1) 약재

시약 및 기기 2)

실험방법 2.

검액 조제 1)

암세포에 대한 독성 2)

통계 처리 3.

암세포주에 대한 물 추출물의 세포 생장 억제 1.

효과

암세포주에 대한 분획물의 세포

2. n-Hexane

생장 억제 효과

Fig. 1. The Viability of Cells was Measured by MTT Activities in Water Extract of Anemarrhenae Rhizoma on Cancer Cell Lines, L-1210 and S-180. * Statistically different(p<0.05) when compared with another group of same concentration.

0 2 0 0 4 0 0 6 0 0 8 0 0 1 0 0 0

0 2 0 4 0 6 0 8 0 1 0 0

% o f S u rv iv a l

C o n c e n t r a t i o n s ( µ g / m l )

L - 1 2 1 0 S - 1 8 0

* *

암세포주에 대한 분획물의 세 3. Ethyl acetate

포 생장 억제 효과

Fig. 2. Microscopic View of Anti-cancer Activities in Water Extract of Anemarrhenae Rhizoma on Cancer Cell Lines, L-1210 and S-180. Control, Normally Grown up Cells; Sample, Water Extract of Anemarrhenae Rhizoma Treated Cells.

L - 1 2 1 0 S - 1 8 0

C o n t r o l C o n t r o l

S a m p l e S a m p l e

Fig. 3. The Viability of Cells was Measured by MTT Activities in n-hexane Fraction of Water Extract of Anemarrhenae Rhizoma on Cancer Cell Lines, L-1210 and S-180.

0 2 0 0 4 0 0 6 0 0 8 0 0 1 0 0 0

0 2 0 4 0 6 0 8 0 1 0 0

L - 1 2 1 0 S - 1 8 0

% o f S u rv iv a l

C o n c e n t r a t io n s ( µ g / m l)

암세포주에 대한 분획물의 세포 생

4. Butanol

장 억제 효과

Fig. 4. The viability of Cells was Measured by MTT Activities in Ethyl Acetate Fraction from Water Extract of Anemarrhenae Rhizoma on Cancer Cell Lines, L-1210 and S-180. * Statistically different(p<0.05) when compared with another group of same concentration.

0 2 0 0 4 0 0 6 0 0 8 0 0 1 0 0 0

0 2 0 4 0 6 0 8 0 1 0 0

L - 1 2 1 0 S - 1 8 0

% o f S u rv iv a l

C o n c e n t r a t i o n s ( µ g / m l )

*

Fig. 5. The Viability of Cells was Measured by MTT Activities in Butanol Fraction from Water Extract of Anemarrhenae Rhizoma on Cancer Cell Lines, L-1210 and S-180. * Statistically different(p<0.05) when compared with another group of same concentration.

0 2 0 0 4 0 0 6 0 0 8 0 0 1 0 0 0

0 2 0 4 0 6 0 8 0 1 0 0

L - 1 2 1 0 S - 1 8 0

% o f S u rv iv a l

C o n c e n t r a t i o n s ( µ g / m l )

*

암세포주에 대한 수용성 분획물의 세포 생장 5.

억제 효과

Fig. 6. The Viability of Cells was Measured by MTT Activities in H

O Fraction from Water Extract of Anemarrhenae Rhizoma on Cancer Cell Lines, L-1210 and S-180.

0 2 0 0 4 0 0 6 0 0 8 0 0 1 0 0 0 0

2 0 4 0 6 0 8 0 1 0 0

L - 1 2 1 0 S - 1 8 0

% o f S u rv iv a l

C o n c e n tra tio n s (µ g /m l)