Theories and Applications of Chem. Eng., 2014, Vol. 20, No. 2 1528
화학공학의 이론과 응용 제20권 제2호 2014년
Engineering of ω-Transaminase for Synthesis of Aromatic Amino Acids
박새롬, 동주영, 신종식*
연세대학교 (enzymo@yonsei.ac.kr*)
Recently, ω-transaminase (ω-TA) is increasingly applied to the synthesis of unnatural amino acids. However, substrate specificity is limited by severe steric hindrance in a small substrate-binding pocket precluding entry of bulkier than a ethyl substituent. Here, we engineered an ω-TA to endow the enzyme with capability of producing aromatic amino acids. Through site-directed mutagenesis of the active site residues based on homology structure modeling, we enlarged the small pocket enough to accept even a benzyl substituent.
