IEG 환경지질연구정보센터

한국지하수토양환경학회 추계학술발표회 및 통합 10주년 기념 국제심포지엄 2010년 10월 14-15일 한양대학교

공업폐수 활성슬러지에서 분리된 신종 클로로페놀 분해균주 Georgenia daejeonensis의 분리 및 동정

Georgenia daejeonensis. sp. nov., a 4-chlorophenol-degrading bacterium isolated from activated sludge of industrial wastewater

Sung-Geun Woo^1,2․YINGSHUN CUI³․Myung-Suk Kang³․Long JIN³․Kwang Kyu Kim⁴․ Myungjin Lee²․Joonhong Park¹ and Sung-Taik Lee³

1School of Civil and Environmental Engineering, Yonsei University, ²Research and Development Division, H-Plus Eco Ltd., ³Department of Biological Sciences, KAIST ⁴Korean Collection for Type

Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology e-mail: Joonhong Park : [email protected]

Sung-Geun Woo : [email protected]

Abstract

A Gram-positive, rod-shaped, aerobic bacterial strain, designated SG01^T, was isolated from activated sludge of industrial wastewater treatment in Daegu, South Korea. SG01^T was characterized taxonomically by using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain SG01^T belongs to the family Bogoriellaceae, class Actinobacteridae, and is related most closely to Georgenia muralis 1A-C^T (97.6 % sequence similarity), Georgenia ruanii YIM 004^T (96.6 %) and Georgenia thermotolerance TT02-04^T (96.8 %). The G+C content of the genomic DNA of strain SG01^T was 64.2 mol%. The detection of quinone system with menaquinone MK8(H4) as the predominant components and a fatty acid profile with anteiso-C15:0, C16:0, iso-C15:0, anteiso A-C15:1, C18:0, iso-C14:0, and anteiso-C13:0 as the major components supported the affiliation of strain SG01^T to the genus Georgenia.

However, the DNA-DNA relatedness between strain SG01^T and the type strains of 3 closely related species of the genus Georgenia ranged from 17 to 29 %, showing clearly that the isolate constitutes a new genospecies. Strain SG01^T could be clearly differentiated from its phylogenetic neighbours on the basis of some phenotypic, genotypic and chemotaxonomic features. Therefore, strain SG01^T is considered to represent a novel species of the genus Georgenia, for which the name Georgenia daejeonensis sp.

nov. is proposed.

Acknowledgement

This work was supported by the GAIA Project(173-091-006), Ministry of Environment, Republic of Korea.

Key words : Georgenia, chlorophenol-degrading bacterium, 16S rRNA, characterization, fatty acid