Korean Journal of Food Science and Technology

(1)

KOREAN J FOOD SCI. TECHNOL. Vol.

22.

No.7, pp. 802~804 (1990)

Starch-iodine Complex£!

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OI%f1 a-Cyclodextrin£!

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Colorimetric Determination of a-Cyclodextrin by Using the Decoloration

of Starch-iodine Complex

In-Yeong Suh, Chul-Sung Huh and In-Kyu Hwang Hankuk Yakult Institute

Abstract

A new colorimetric method to analyze a-cyclodextrinCa-CD) by using a property of a-CD to decolor the starch-iodine complex, was presented. a-CD was quantitatively determined as follows. The a-CD standard solutions at concentrations up to 2 mg/ml were prepared. To 1 ml of starch-iodine complex

solution which contained starch(1%) and iodineCO.02% h. 0.2% KI) equivalently in distilled water,

200 III

of a-CD standard solutions and 3 ml of distilled water were added and the absorbance was

measured at 570 nm, Using this method a-CD concentration range at 0~2 mg/ml could be determined

and their absorbance patterns at 570 nm showed a good linearity. P-CD and glucose had no interference in this method for a-CD determination.

Key words: a-cyclodextrin. colorimetric determination. starch-iodine complex

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Corresponding author : Y eong Suh. Hankuk Yakult

In-stitute, Wangok-Dong. Euiwang-Si. Kyunggi-Do 449

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%<if ~~-{! ~~q]j 1 mlol1 a-CD 1iT%olj 200 Ill~

~a7}i>}2, r:pl %*4- 3 ml~ 7}13l9 Gilford Response Spectrophotometer( Gilford Ins., Lab .. USA) ~ 01 %i>}9

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(2)

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- - - , - - - - , - - - - . , - - - - , - - - - 1

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Fig. l. Absorption spectra of iodine-starch complex ( - -) and a-CD treated iodine-starch complex( -. - ) 1 . 8 , - - - , II

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Fig. 2. Absorption spectra of iodine( - -) and

a-CD-iodine complex ( - . - )

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Fig. 3. Absorption spectra of iodine-starch complex

treated with a-CD at various concentrations - - : 0 mg/ml. -. - : 0.5 mg/ml • .... : 1.0 mg/mi . • : 1.5

mg/mi . • : 2.0 mg/mi

l:i!} Il-c *%.5:.7} ~7}i5t-c 'li~ *% peak III~ * %.5:.-c 7J5:~4.

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a-804

Table 1. Statistical data for linearity between a-CD concentration and absorbance at 570 nm

a-CD cone. (mg/mI)range 0-5.0 0-3.5 0-2.0 Correlation -0.981 -0.992 -0.998 Slope Error -0.0020 0.414 -0.0025 0.085 -0.0029 0.041

Table 2. Statistical data for the interference of glucose

in the a-CD determination

- - - -

---~----Glucose conc.

Correlation Slope Error

(mg/mI)

0 -0.999 -0.0028 0.021

5 -0.999 -0.0028 0.019

10 -0.999 -0.0028 0.024

20 -0.999 -0.0028 0.024

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Aa2J'

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Table 3. Statistical data for the interference of ~-CD

m the a-CD determination ~-CD conc.

Correlation Slope Error

(mg/m!) 0 -0.998 -0.0026 0.027 0.25 -0.998 -0.0029 0.030 0.50 -0.999 -0.0028 0.027 1.00 -0.998 -0.0028 0.035

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1. French. D. : The Schardinger dextrins. Adv. Carbohydr . Chern .. 12, 189(1957)

2. Zeadon. B., Otta, K.H. and Tudos, F,]. : Separation of cyclodextrins by high-performance liquid chromatogra-phy.

J

Chromatogr., 172, 490(1979)

3. Hokse, H.: Analysis of cyclodextrins by high-perfor-mance liquid chromatography. J Chromatogr., 189, 98 (980)

4. Kitahata, S., Yoshikawa, S. and Okada, S.]. : Determina-tion of U', ~- and y-cyc1odextrin by high performance liquid chromatography.

J

jpn. Soc. Starch Sci., 25, 19 (1978)

S. Kato, 1'. and Horikoshi, K. : Colorimetnc determination of y·cyclodextrin. Anal. Chern., 56, 1738(1984) J. Hirai, H., Toshima, N. and Uenoyama, S.: Inclusion

complex formation of cyclodextrin with large dye mo-lecule. Polymer journal, 13, 607(1981)