L. Increased multipolar spindle in anti-KIFC1 scFv expressing cells
V. CONCLUSION
We found that variable regions of cytosolic IgG intrabodies determine H:L association and antigen-binding activity. Also, partial intra-chain disulfide bonds in the cytosolic IgG intrabodies were closely related to the integrity of constant region. Cytosolic antibody in a scFv form showed antigen-binding activity and could inhibited the function of target protein.
This is the first report to investigate structural properties of cytosolic IgG intrabody.
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