Overexpression of EFGR as Prognostic Factor and Effect of EGF in the Progression of Hypopharyngeal Cancer

-  - 대한 두경부 종양 학회지

제  권 제  호 

Overexpression of EFGR as Prognostic Factor and Effect of EGF in the Progression of Hypopharyngeal Cancer

Young Chang Lim, MD

, Eun Chang Choi, MD, PhD

, Yoon Tae Kim, MD

, Jang Hee Kim, MD, PhD

, Hye Sook Hwang

, Sung Un Kang, PhD

, Jae Won Chang, MD

, Yoo Seob Shin, MD

, Chul-Ho Kim, MD, PhD

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하인두암에서 예후인자로서의 표피성장인자수용체(EGFR) 과발현과 하인두암의 진행에 있어 표피성장인자(EGF)의 역할 분석

건국대학교 의과대학 이비인후-두경부외과학교실,¹ 연세대학교 의과대학 이비인후과학교실,²

아주대학교 의과대학 이비인후과학교실,³ 세포사멸조절연구센터,⁴ 아주대학교 의과대학 병리과학교실⁵

임영창¹・최은창²・김윤태^3,4・김장희⁵・황혜숙^3,4・강성운^3,4・장재원^3,4・신유섭^3,4・김철호^3,4

= 국 문 초 록 =

연구배경 및 목적

표피성장인자수용체(EGFR)는 HER2/neu(erbB2), HER3(erbB3), HER4(erbB4)를 포함하는 receptor tyrosine ki

nase의 erbB 그룹에 속하는 수용체이다. 표피성장인자수용체의 과발현은 다양한 종류의 암, 특히 두경부편평세포암 에서 예후를 악화시킨다고 알려져 있다. 이에 저자들은 하인두편평세포암에서 표피성장인자수용체의 발현 및 분포를 확인하고, 하인두암에서 표피성장인자(EGF)가 암세포의 증식과 침습에 미치는 영향에 대해 알아보고자 하였다.

대상 및 방법

57명의 하인두편평세포암 환자의 조직에서 표피성장인자수용체의 발현을 면역화학적염색을 통해 확인하고, 이에 대해 임상병리학적 요인과 생존율에 대한 분석을 시행하고, 일부 환자의 정상 및 암조직에서 Western blot을 시행하 였다. 하인두편평세포암 세포주인 FaDu에서 proliferative assay, colony dispersion, wound healing assay, invasion assay를 시행하여, 하인두암의 진행에서 표피성장인자의 역할에 대하여 분석하였다. 또한 RT-PCR과 Zymography를 통하여 Matrix metalloproteinase(MMP)-2, 9의 발현을 확인하였다.

결 과

63.2%의 하인두편평세포암 조직에서 표피성장인자수용체의 발현이 확인되었다. 표피성장인자수용체의 발현은 정상조직에서 비하여 하인두암 조직에서 유의하게 증가되어 있었으며, 병리학적 병기(S=0.022)가 올라갈수록 유의 하게 증가하였으나, 증례수의 제한으로 생존율에서는 통계적 유의성을 얻지는 못했다(S=0.053). LQYLWUR의 결과로 표피성장인자를 FaDu 세포주에 처리하였을 때, FaDu 세포주의 증식이 유의하게 증가되었으며(S＜0.05), Transwell invasion chamber상 침습의 증가가 확인되었다(S＜0.05). RT-PCR과 zymogram 실험상 표피성장인자처리시 FaDu

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교신저자 : 김철호, 442-749 경기도 수원시 영통구 원천동 산 5 아주대학교 의과대학 이비인후과학교실 전화 : (031) 219-5269 ・ 전송 : (031) 219-5264 ・ E-mail : ostium@ajou.ac.kr

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Introduction

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Materials and Methods

1. Patients

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세포주의 MMP-2, 9이 발현이 증가되고 활성화되는 것이 확인하였다.

결 론

본 연구에서 표피성장인자수용체의 과발현이 하인두암의 예후 인자로서의 가능성을 확인하였고, 표피성장인자가 하인두편평세포암의 증식과 침습에 관여하는 것을 확인하였다.

중심 단어：표피성장인자수용체・표피성장인자・하인두편평세포암・침습・예후.

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2. Cell culture

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3. Immunohistochemistry

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4. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis

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5. Western blot analysis

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6. Proliferation assay

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7. Colony dispersion analysis

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8. Wound healing assay

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9. Invasion assay

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