The effect of the stem of Spatholobus suberectus Dunn on the proliferation and gene expression related apoptosis in human cervical cancer cells

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The effect of the stem of Spatholobus suberectus Dunn on the proliferation

and gene expression related apoptosis in human cervical cancer cells

Byounghoe Kim, Seunghee Baek

Dept. of gynecology, College of Orinental Medicine, Daegu Haany University 대구한의대학교 한의학과 부인과교실

김병회 백승희,

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이 자궁경부암세포의 세포자멸사 유도와 관련 유전자 발현에 미치는 영향 鷄血藤

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0 0 .2 0 .4 0 .6 0 .8 1 1 .2

Cell numbers (ratio)

C o ntro l 10 0 2 00 3 0 0

* * * *

*

0 0 .2 0 .4 0 .6 0 .8 1 1 .2

Cell numbers (ratio)

C o ntro l 10 0 2 00 3 0 0

* * * *

*

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C o n trol C o n trol

1 0 0 ㎍ /㎖

1 0 0 ㎍ /㎖ 2 0 0 ㎍ /㎖2 0 0 ㎍ /㎖ ^{3 0 0 ㎍ /㎖}^{3 0 0 ㎍ /㎖}

1 4 . 9 1 8 . 3 9 3 0 . 4 3 4 5 . 9 9 3 0 0 ㎍ / ㎖

1 5 . 8 4 1 2 . 0 6

1 0 . 6 3 G 2 / M

9 . 7 1 9 . 4 8

1 7 . 8 4 S

2 9 . 7 4 3 1 . 5 6

5 2 . 2 6 G 1

4 3 . 5 7 4 3 . 7 5

1 9 . 0 6 S u b G 1

2 0 0 ㎍ / ㎖ 1 0 0 ㎍ / ㎖

C o n t r o l

1 4 . 9 1 8 . 3 9 3 0 . 4 3 4 5 . 9 9 3 0 0 ㎍ / ㎖

1 5 . 8 4 1 2 . 0 6

1 0 . 6 3 G 2 / M

9 . 7 1 9 . 4 8

1 7 . 8 4 S

2 9 . 7 4 3 1 . 5 6

5 2 . 2 6 G 1

4 3 . 5 7 4 3 . 7 5

1 9 . 0 6 S u b G 1

2 0 0 ㎍ / ㎖ 1 0 0 ㎍ / ㎖

C o n t r o l

( % g a t e d )

P r o c a s p a s e 3 ( 3 2 k D a )

P A R P ( 1 1 6 / 8 5 k D a )

β- t u b u l i n ( 4 6 k D a )

1 1 6 k D a 8 5 k D a

R b ( 1 1 6 k D a ) P r o c a s p a s e 3 ( 3 2 k D a )

P A R P ( 1 1 6 / 8 5 k D a )

β- t u b u l i n ( 4 6 k D a )

1 1 6 k D a 8 5 k D a

R b ( 1 1 6 k D a )

β

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P 2 1 ( 2 1 k d a )

P 5 3 ( 5 3 k D a ) P 2 7 ( 2 7 k D a )

C y c l i n A C y c l i n D 1

C y c l i n E β - t u b u l i n ( 4 6 k D a ) P 2 1 ( 2 1 k d a )

P 5 3 ( 5 3 k D a ) P 2 7 ( 2 7 k D a )

C y c l i n A C y c l i n D 1

C y c l i n E β - t u b u l i n ( 4 6 k D a )

β

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