Effects of Herbal-Acupuncture with Evodiae Fructus at KI10 on Osteoporosis in Ovariectomized Mice

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경락경혈학회지 Vol.27, No.2, pp.217 242, 2010∼ Korean Journal of Acupuncture

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Sung-Hoon Choi , Byung-Ryul Lee , Gi-Young Yang Jae-Kue Kim , Young-Suk Seo , Yun-Kyoung Yim

Dept. of Meridian & Acupoint, College of Oriental Medicine, Daejeon University

Division of Clinical Medicine, School of Korean Medicine, Pusan National University, Miz Woman's Hospital

Abstract

Objective : This study is aimed to evaluate the effects of Evodiae Fructus herbal-acupuncture (EF-HA) at KI10 on osteoporosis induced by ovariectomy in mice.

Method : Mice underwent bilateral ovariectomy. After recovering, the ovariectomized (OVX) mice were treated by needle prick, saline injection, herbal acupuncture with Evodiae Fructus (EF-HA) at KI10 for 8 weeks.

Result :

1. EF-HA at KI10 significantly inhibited the overgrowth of tibia in ovariectomized mice.

2. NP at KI10 significantly restored the tibial BMD (bone mineral density) in ovariectomized mice.

3. EF-HA at KI10 significantly restored the phosphorus and creatinine levels in ovariectomized mice serum.

4. EF-HA at KI10 significantly restored the tibial Ca and P levels in ovariectomized mice.

5. EF-HA at KI10 significantly reduced the tibial osteoclast-like cells in ovariectomized mice.

6. EF-HA at KI10 significantly inhibited the overgrowth of tibial GPL (growth plate length) in ovariectomized mice.

Conclusion : EF-HA at KI10 has protective and therapeutic effect for osteoporosis in ovariectomized mice. Thus, it is suggested that EF-HA can be an useful therapeutics in clinical field after further researches.

Key words : Osteoporosis, Herbal-acupuncture, Evodiae Fructus, KI10

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Scheme 1. Manufacturing procedure of EF-HA Solution.

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EF-HA

4 5 6 7 8 9 10 11 12 13 14 15 16 ( Weeks )

Surgery (OVX) Sacrificed

EF-HA

4 5 6 7 8 9 10 11 12 13 14 15 16 ( Weeks )

Surgery (OVX) Sacrificed

Scheme 2. Osteoporotic mouse model

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Fig. 1. Cell viability rate with various concentrations of EF-HAS.

Fig. 2. Effect of EF-HA on body weight of ovariectomized mice.

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- 225 - Fig. 3. Effect of EF-HA on the uterine weight of ovariectomized mice.

Fig. 4. Effect of EF-HA on the uterus index in ovariectomized mice.

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Fig. 5. Effect of EF-HA on the tibial length in ovariectomized mice.

Fig. 6. Effect of EF-HA on tibial BMD in ovariectomized mice.

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Table 1. Effect of EF-HA on various components in serum of ovariectomized mice.

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Table 2. Effect of EF-HA on Ca, P, Ca/P ratio in tibia of ovariectomized mice.

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- 229 - Fig. 7. Effect of EF-HA on the weight of tibia ash in ovariectomized mice.

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Fig. 8. Effect of EF-HA on osteoclast like cell in tibia of ovariectomized mice.

Fig. 9. Effect of EF-HA on osteoclast like cell in tibia of ovariectomized mice.

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- 231 - Fig. 10. Histological analysis (H&E stain) of tibia in ovariectomized mice.

Fig. 11. Effect of EF-HA on the trabecular bone volume of tibia in ovariectomized mice.

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Fig. 12. Effect of EF-HA on the trabecular bone thickness of tibia in ovariectomized mice.

Fig. 13. Effect of EF-HA on growth plate length of tibia in ovariectomized mice.

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