Antipruritic Effects of Puerariae Radix on 5-HT or AEW-Induced Itch Behavior in Mice

서 론

( ,

, )

, ( , )

.

,

2007 8 12 2007 8 14

2007 8 25

: ,

(Tel : 02-961-0419, Fax : 02-964-0325, E-mail: [email protected])

搔痒症 葛根 鎭痒

한재복

, 선보람

, 부영민

, 김호철

원 저

Antipruritic Effects of Puerariae Radix on 5-HT or AEW-Induced Itch Behavior in Mice

Jae-bok Han ^1,2 , Bo-ram Sun ¹ , Yung-min Bu ² , Hoc-heol Kim ²

1

Department of East-West Medicine, Graduate School

2

Department of Herbal Pharmacology, College of Oriental Medicine

Objectives : To find antipruritic herbal medicines, we screened the water extracts of four herbal medicines which have been frequently prescribed to treat dermatologic diseases in oriental medicine.

Methods : Water extracts of Bupleuri Radix, Scutellariae Radix, Cimicifugae Rhizoma and Puerariae Radix were administered at a dose of 300 mg/kg to male ICR mice. 1 hour later, serotonin hydrochloride dissolved in physiologic saline at a concentration of 100 nmol / 50 was administered intradermally to the nape of the neck at a dose of 50 . Then the scratching behavior was observed. Dry skin pruritus was induced with cutaneous application of acetone / ether (1:1) mixture and water (AEW) to the rostral back of male ICR mice, twice a day for 5 days. 14 hours after the last application, the most effective material in the first experiment was administered perorally at doses of 75, 150, 300, 600 and 1200 mg/kg. 1 hour later, the scratching behavior was observed.

Results : Water extracts of Puerariae Radix significantly inhibited the serotonin-induced scratching behavior and the mean of scratching bouts was reduced by half compared with the control group. Bupleuri Radix group also showed a 29% decrease in the mean of scratching bouts, but there was no statistical significance. Water extracts of Puerariae Radix also inhibited the AEW-induced scratching behavior, in a dose-dependent manner. The dose of 150 mg/kg showed the highest and statistically significant antipruritic effect.

Conclusions : These results suggest that Puerariae Radix and its constituents have antipruritic effect, and are new candidates as antipruritic agents.

Key Words : herbal medicine; puerariae radix ; itch; scratching; serotonin; dermatitis

(dermo-epidermal junction) (itch receptors)

(free unmyelinated nerve endings)

1)

. (pruritogens)

(dorsal horn) (spinothal-

amic tract) (thalamus)

(cortex cerebri)

.

.

, H1

,

.

,

,

. ,

,

. ,

,

. , (5-HT),

compound 48/80 substance P

3,4,5)

. ,

(lipid)

/ (AEW)

6)

.

,

( ),

( )

( , Bupleuri Radix), ( , Scutellariae Radix), ( , Cimicifugae Rhizoma), ( , Puerariae Radix)

( ) 5-HT

,

AEW

.

재료와 방법

1.

재료 1)

4~6 , 28~34g ICR

. 25±2 , 08:00~20:00 .

. National

Institutes of Health ‘Guide for the Care and Use of Laboratory Animals’

. 2)

Bupleuri Radix, Scutellariae Radix, Puerariae

Radix , Cimicifugae Rhizoma

2007 5 ( ) .

600g 3,000cc 2

.

2,000cc 2

, (EYELA Co.,

Model NE-1, ) ,

(EYELA Co., Model FD-1, ) .

24.8%, 48.8%, 22.7%, 41.3%

.

. 3)

5-HT hydrochloride Sigma(U.S.A.)

(0.9%

NaCl) 100nmol/50

7)

.

( ) .

2.

방법 1) 1

2% 1kg 300mg

10g 150

(p.o.) . 2 1

1kg 75, 150, 300, 600 1,200mg 1, 2, 4, 8 16%

, 10g 75

.

. 10

.

2) 5-HT AEW

2×2cm

. 1

1 5-HT 2% 50

(I.D.)

.

2 , 1:1

2x2cm

15 ,

30 .

AEW 9 5

1 , 2 , 5

. 14

6)

.

3)

1 1

5-HT , , ×

× 10×5×27cm

,

60 .

.

2 1

.

4)

8)

,

1 , 60

. unpaired

t-test , p value 0.05

* . data mean ±S.E.M.

.

결 과

, , , 300 mg/ kg

1 , 5-HT

(100nmol / 50 physiologic saline) 50 1

1 ,

. 29%

.

(Fig. 1, Table 1).

AEW 2 , 5

2 ,

75mg/kg

23%

, 150mg/kg

70%

. 300, 600, 1,200mg/kg 150mg/kg

50%

(Fig. 2, Table 2).

80 70 60 50 40

20 10 0

Scratching Bouts / 60 min

Physiologic Saline

*

*

*

*

Bupleuri Radix

Scutellariae Radix

Cimicifugae Rhizoma

Pueranae Radix

Herbal Medicine Scratching Bouts/1hr

Physiologic Saline 61.70 ± 11.72

Bupleuri Radix 43.50 ± 9.026

Scutellariae Radix 54.70 ± 7.520

Cimicifugae Rhizoma 63.60 ± 14.61

Puerariae Radix 29.90 ± 5.555 *

고 찰

, , 5-HT

(antipruritic effect) .

,

2 AEW

, 150mg/kg .

1 300mg/kg

,

50%

, 30%

.

. ,

.

,

, 300mg/kg

1kg

1,209mg, 614mg, 1,321mg,

726mg . 70kg

1 84.63g, 42.98

g, 92.47g, 50.82g 1 .

.

Dose(mg/kg) Vehicle 75 150 300 600 1,200

Scratching

Bouts/1hr 37.50 ± 11.24 28.90 ± 9.627 10.90 ± 5.174 * 18.60 ± 7.245 17.80 ± 3.852 18.70 ± 7.906

50

40

30

10

0

Scratching Bouts / 60 min

Vehicle

20

* * * *

75 150 300 600 1200

Puerariae Radix (mg/kg)

5-HT

, 5-HT2

7,9)

. ,

puerarin 5-HT

5-HT1A 5-HT2A

10)

.

,

(hippocampus) (striatum) norepin- ephrine 4-dihydroxyphenylacetic acid(DOPAC,

dopamine )

11)

. Puerarin in vitro 5-HT ADP ,

5-HT

12)

. ,

, , 5-HT ,

,

.

2 300mg/kg

50% 1

,

AEW 5-HT

.

AEW ,

6)

, 2 5

5-HT

,

. 600mg/kg, 1,200mg/kg

, 150mg/kg

70%

, 75mg/kg 30%

.

.

,

. ,

13,14,15,16)

.

.

.

결 론

, ,

5-HT

, 1:1

(AEW)

. 1. 5-HT

, , ,

. 2. AEW

, 75,

150, 300, 600 1,200mg/kg , 150mg/kg

.

5-HT

.

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