Effect of the Combination of Total Saponin of Red Ginseng and Coisis Semen for the Prevention and Treatment of Obesity

서 론

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紅蔘 薏苡仁 肥滿

김인경, 민상연, 김장현 동국대학교 한의과대학 소아과학교실

Original Article

Effect of the Combination of Total Saponin of Red Ginseng and Coisis Semen for the Prevention and Treatment of Obesity

In-Kyoung Kim, Sang-Yeon Min, Jang-Hyun Kim

Department of Pediatrics, College of Oriental Medicine, Dongguk University

Objectives: This study was on the pharmaceutical components in purified ginseng total saponin (GTS), coisis semen (CS), the combination of these drugs, and the major component of coicis coixol for the prevention and treatment of obesity.

Methods: In this study, to evaluate the effect on the suppression of obesity, high fat diet-induced obese rats were treating with the drugs, the effects on the balance of energy and diet activity were examined, and the change of weight, the change of the intake of diet, body fat rate, etc. were assessed.

Results: The results demonstrate that in high fat diet-induced obese white rats, the combination treatment of ginseng total saponin and coicis was effective in suppression of weight gain, reduction of intake of food, and reduction of body fat.

Conclusions: The results suggest that a combination treatment with major components of red ginseng total saponin and coicis may be used therapeutically for the suppression and treatment of obesity.

Key Words : Total saponin of red ginseng, coisis semen, coicis coixol, obesity

접수：2008년 6월 26일 수정：2008년 9월 2일 채택：2008년 9월 5일

교신저자：김장현(Jang-Hyun Kim)

경북 경주시 석장동 1090-1번지 동국대학교 경주한방병원 소아과, 동국대학교 한의학연구소 Tel：+82-54-770-1260, Fax：+82-54-770-2281, E-mail：[email protected]

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(ginseng total saponin, GTS) (Coicis Semen, CS),

(coixol)

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재료 및 방법

1. 재료 1)

80±10g(3 ) Sprague-Dawley (Orient Co. Institute, Korea) .

1

, 5 (High fat diet,

HF: AIN-76A diet #100496, Dyets Inc., Bethlehem,

PA, USA) . 7

Ingredients High fat diet

Casein 200

DL-Methionine 3

Corn Starch 150

Sucrose 345

Cellulose 50

Corn Oil -

Beef Tallow 205

Salt Mixture 35

Vitamin Mixture 10

Choline Bitartrate 2

Fat % (Calories) 40.0

High fat diet: AIN-76 diet #100496 (Dyets Inc., Bethlehem, PA, USA)

3 (ginseng total saponin, GTS), (Coicis Semen, CS),

(coixol) .

(

23±3 , 50±10%), 12

,

. 5 .

Table 1 .

2)

5 ,

, (ginseng total saponin,

GTS), (Coicis Semen, CS) (GTS-CS) (coixol)

. 3)

6 42

80mesh (

7.22%) .

acetonitrile, n-butanol, met-

hanol, E. Merck HPLC

,

. 85%

, Sigma

.

70%

pH

3 .

Ando n-

, ,

. Ko

HPLC ginsen-

oside-Rb1, -Rb2, -Rd

. (1ml/kg, i.p.),

(200mg/kg, i.p.), (100mg/kg, i.p.),

(1mg/kg, i.p.) .

2. 방법 1) ,

2 ,

,

.

(epididymal

fat) ,

(peritoneal fat) (visceral fat) .

2)

± (mean±S.E.)

. ANOVA(analysis of variance)

Tukey’s test

. p<0.05

. SPSS (version 8.0)

.

결 과

1. 체중의 변화

,

. (425.6±20.3)

GTS(310.8±8.5) 73.0%

(p<0.05) , GTS-CS (296.0±10.1) 69.5%

(p<0.01) (Table 2).

Day Normal Diet GTS CS GTS-CS Coixol

12 107.0±4.8 105.6±2.9 102.8±2.2 101.8±3.4 105.6±5.1

15 135.0±5.3 131.0±3.6 126.0±3.1 125.0±4.8 135.0±4.8

18 159.2±8.2 155.6±4.3 155.0±3.8 156.6±6.3 167.8±11.2

21 203.8±8.9 204.8±6.0 197.8±4.7 198.8±7.5 203.6±11.0

24 217.8±10.9 229.4±7.1 217.2±5.9 223.0±6.8 221.0±12.0

27 273.6±12.3 267.0±7.6 256.6±9.1 266.6±4.3 270.4±9.5

30 295.2±12.9 294.0±7.8 280.8±10.8 286.6±7.8 300.2±7.7

33 303.8±15.0 315.6±9.3 299.8±11.9 305.0±9.1 318.2±14.2

36 340.8±15.7 338.0±10.7 321.0±14.2 334.2±4.7 341.6±12.0

39^# 355.4±16.0 357.4±11.1 341.6±17.1 342.0±8.8 358.6±6.8

42 365.8±17.6 332.2±14.4 345.8±6.6 315.8±11.8^* 371.8±11.0

45 381.4±16.7 309.2±13.4^* 359.6±8.8 284.3±7.5^** 358.6±19.4

48 396.0±17.3 307.0±13.6^* 365.4±7.3 282.7±3.0^** 376.0±22.3

51 401.6±17.7 305.4±19.5^** 368.6±6.7 273.0±8.9^** 385.0±14.1

54 411.8±19.3 295.5±6.8^** 365.2±7.0 281.5±13.6^** 414.6±12.0

57 425.6±20.3 310.8±8.5^* 387.4±8.1 296.0±10.1^** 423.6±12.2

rate 100% 73.0% 91.0% 69.5% 99.5%

#

, Start of Treatment

*

, P<0.05,

, P<0.01 compared with normal diet group.

Normal diet: Group of rats fed on HF diet with saline treatment.

GTS: Group of rats fed on HF diet with ginseng total saponin treatment.

CS: Group of rats fed on HF diet with Coicis Semen treatment.

GTS-CS: Group of rats fed on HF diet with ginseng total saponin-Coicis Semen treatment.

coixol: Group of rats fed on HF diet with coixol treatment.

Table 2.

day Normal diet GTS CS GTS-CS coixol

6 13.2 13.1 12.1 12.3 8.2

9 13.6 13.3 11.8 12.1 10.4

12 16.9 15.9 16.7 16.3 8.2

15 16.7 15.8 14.7 14.9 14.3

18 17.9 16.7 16.5 14.9 12.5

21 20.5 17.5 17.5 15.9 15.2

24 21.5 18.7 21.0 15.6 12.9

27 19.4 20.0 19.5 18.0 17.0

30 19.1 19.7 20.2 18.2 18.0

33 18.1 20.4 18.9 17.9 16.0

36 19.4 18.3 19.9 17.3 16.0

39^# 18.7 18.5 17.6 18.4 15.0

42 17.1 7.7^** 12.8 7.4^** 13.5

45 18.6 4.8^** 15.7 1.9^** 13.7

48 15.1 3.6^** 11.8 4.2^** 12.3

51 14.5 4.5^** 13.4 5.0^** 13.7

54 15.1 10.1^* 14.3 11.2^* 21.1

57 14.5 12.8 13.3 12.5 17.0

Total food intakes(g)

929.5 100%

754.2 81.1%

862.9 92.8%

701.6 75.5%

764.7 82.3%

Diminishment 18.9% 7.2% 24.5% 17.7%

#

, Start of Treatment

The weight of food intakes has been measured everyday, however, this table presents only the values of every 3 day for convenience.

*

, P<0.05,

, P<0.01 compared with normal diet group.

Table 3.

2. 식이섭취량의 변화

GTS 18.9%,

GTS-CS 24.5% .

, GTS GTS-CS

(p<0.05)

, 2 GTS GTS

-CS (p<0.01)

(Table 3).

3. 체지방량의 변화

(epididymal fat)

GTS(9.6±0.4) 31.1%, GTS-CS(10.2±3.3) 27.4%

(p<0.05) , (visceral

fat) GTS(8.5±1.9) 66.8%, GTS

-CS(7.4±2.7) 71.1% (p<0.01)

, (peritoneal fat)

CS(20.7±2.4) 22.2% (p<0.05), GTS-CS(7.7±0.7)

68.9% (p<0.01) (Table 4).

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Group Normal diet GTS CS GTS-CS coixol

Epididymal fat/

B.W. ㎏(g) 16.3±1.7

100%

9.6±0.4^* 58.9%

14.2±1.4 87.1%

10.2±3.3^* 62.6%

19.0±1.2 116.6%

Visceral fat/

B.W. ㎏(g) 25.6±4.3

100%

8.5±1.9^**

33.2%

17.5±0.9 68.4%

7.4±2.7^**

28.9%

27.8±2.7 108.6%

Peritoneal fat/

B.W. ㎏(g) 26.6±4.7

100%

21.0±9.5 79.0%

20.7±2.4^* 77.8%

7.7±0.7^**

31.1%

28.1±2.7 1105.6%

*

, P<0.05,

, P<0.01 compared with normal diet group.

Table 4.

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