Evaluation of micronucleus frequency in cytokinesis-blockedlymphocytes of cattle in the vicinity of Uljin nuclear power station

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(3). *ÎL >~ ö¶K~ö (²Òß: 2004j 7ú 1¢) 1. Evaluation of micronucleus frequency in cytokinesis-blocked lymphocytes of cattle in the vicinity of Uljin nuclear power station Se-ra Kim, Chang-mo Kang1 and Sung-ho Kim* College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, Korea 1 Korea Institute of Radiological & Medical Science (Accepted= July 1, 2004) Abstract : Cytogenetic and hematological analysis was performed in peripheral blood of cattle in the vicinity of Uljin nuclear power station and control area. The frequency of micronuclei(MN) in peripheral blood lymphocytes from cattle was used as a biomarker of radiobiological effects resulting from exposure to environmental radiation. An estimated dose of radiation was calculated by a best fitting linear-quadratic model based on the radiation-induced MN formation from the bovine lymphocytes exposed in vitro to radiation over the range from 0 Gy to 4 Gy. MN ratio in lymphocytes of cattle from Uljin nuclear power station and control area were 8.90/1,000 and 9.60/1,000, respectively. There were no significant differences in MN frequencies and hematological values in cattle between Uljin and control area. Key words : radiation, micronuclei, cattle, lymphocytes, nuclear power station. * . ' 5 F¿Ú OÒW bî~ FZ¢ 2k~º Ò & "¢ ® [25, 26, 29]. ²öB â* ² G;»f 1993j Scarfi [23]ö ~ . B ¾ OÒF 5 "Öz>²ö ~ ëW þö Ò Ï>î [8] " Ò:ÊjöB æê ~ã J"~ N¢ 2k~º O»b B 'Ï>î [28]. &Ë ô 'Ï>º OÒFb~ b' FïG; f .Ú â*~ Q' æÿ [7] dicentric" centric ring~ êGj 7b "ïÚ ªC» OÒF Ò 'Ï G;>î [3, 10, 11, 22]. ¾ .^~ Q' æzº ö*V^(stem cell) 5 ^W?ê¦V Ú¶ ;ê Ï&Ëæ~ ;ê, *ã" ê~ ^Òö ~ >' ² ~ jN, jË". ö¶KB*²¢ &çb OÒF b' Fï G; f " J «Ò¶¢ &çb ¯>î [5, 16, 27, 30, 31], "ïÚªC»j &~ " ^îª N â*~ ² Bj æ ~ ® [30, 31]. ÿb F¾ ^¢ Ï OÒF b~ b' G; f " "ïÚ~ çFZ 5 ÿb« *~ 6>W N & Ò>îb [12], " *Þ ² Bö & & ê¯>B v &æ ^F*' ªC*~ N 6 rJæ ® [4, 15, 21]. ö* "æ ÿ bj &çb º Úr ö* Ò ê OÒ W bî~ Ú¦J"ö & ËV ê OÒË bî~ » \º 8F¦ ö¶K\**Òë~ æöb >¯>îr.. *Corresponding author: Sung-ho Kim College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, Korea [Tel: +82-62-530-2837, Fax: +82-62-530-2841, E-mail: [email protected]]. 343.

(4) B^¢Á;cÎÁBW^. 344. ?f .FËV ç ~ æzö V .>~~ N Cç~ ^B6 ® [7] "ïÚªC» f Òò~ B· 5 ªCö ôf *" K ®Ú¢ ~ , $ ç >&~ ?NB VF ®Ú¢ > ®. º 6 ® [10, 17]. >^¢ Ï~º *Û ' ² ¦Òö j~ ^îª N â*¢ Ï ² ¦Òº ¢² ª~ { 5 ª^ ~ VB& &Ë~V r^ö *Ò OÒFbª¢öB FïG; 5 >w.bî~ Î" ¦; þö ô ' Ï> ® [1, 9, 14, 17, 21, 32]. ÿbf "* ~ãö Ò~º &æ Fbîö 'Ëj Aº . OÒFj j bÒ' F*Ë FB bî 5 ³£ ~ z' F*Ë FBbîö ~ ò æ, &ÒË, ç, &~ ~ Ãçj ¢ bÒ > ® . ß® jÿbj j &»f *~ ~ãj F º &6öB &ç ¶Ú~ Ë ö ò jî¢ *ö & F¶~ ·Ïj &æ > ® Ú "* ~ã~ FW ï& ª¢öB Ö 7º~. [2, 6]. öBº ²¢ &çb .>~ 5 ^î ª N â*öB BB ²j G;~ Þêö¶ KB*²f &æ~ N¢ &V~&b ² B ;ê¢ þ'b ÖÂB OÒF b Fï->w Fö 'Ï~ º;Fïj 2k~& .. Òò 5 O». . >~ 8. þ&ç ²~ ö..j 2Ö Î&B vacutainer ö j~ ÿb*Ï .ªCV(Hemavet 850+, CDC Technologies Inc., USA)¢ ÒÏ, W., '. 5 .² 6~ ç¢ ¦ÒÏ ê ªC~& . W.º CW ., ^7, ^Ö, ^", , â*¢ 6ê G ;~ C> 5 WªNj ÖÂ~&b, '.º C' ., hemoglobin, hematocrit j ÖÂ~& .. þ^

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(7) î ª N â*\¢ Ï Þêö¶KB*² "æ ²~ ² B ï& Table 1. Hematological values in cattle of Uljin nuclear power station and control region (mean±S.D.) Test Erythrocytes Hemoglobin Hematocrit. Unit. Control region. Uljin region. M/ul g/dL %. 7.16±0.50 9.83±0.52 29.90±1.96. 8.78±1.10 13.52±1.62 43.02±5.26. Thrombocytes. K/ul. Leukocytes Neutrophils Lymphocytes Monocytes Eosinophils Basophils. K/ul K/ul K/ul K/ul K/ul K/ul. 240.14±59.01 278.8±41.82 8.18±1.89 3.37±1.13 4.49±1.47 0.25±0.10 0.13±0.10 0.04±0.03. 8.70±1.51 4.01±1.33 4.14±0.42 0.26±0.14 0.22±0.10 0.07±0.04. 345. Table 2. Micronuclei (MN) per 500 cytokinesis-blocked lymphocytes following irradiation of cattle No. of cells Experimental without group MN. Number of micronuclei per cell 1. 2. 3. 4. Total number of MN. Donor 0 100 200 400. 1 cGy cGy cGy cGy. 493 451 392 279. 7 43 6 80 24 4 173 36 11 1. 7 55 140 282. Donor 0 100 200 400. 2 cGy cGy cGy cGy. 492 452 401 282. 8 44 4 75 19 5 167 47 12 2. 8 52 128 305. Donor 0 100 200 400. 3 cGy cGy cGy cGy. 493 441 384 252. 7 53 6 89 25 2 189 41 17 1. 7 65 145 326. Table 3. Frequency of micronuclei in cytokinesis-blocked (CB) cells following treatment with gammarays (mean±S.D.) Dose(cGy). Micronuclei per CB cell. 0 100 200 400. 0.015±0.001 0.115±0.014 0.275±0.017 0.609±0.044. Fig. 1. Photomicrograph of cytokinesis-blocked lymphocyte containing one micronuclei (arrow). Diff Quik stain, X1000.. ¶Ú~ ^ëWj J~ '³êº 4 µg/ml Û¢~& . *~ öB V·B â*öB â *º £ 16% & . þ' OÒF Òö V ²~ B ·çf Fig. 1, Table 2 5 Table 3f ?b OÒF Òö V¢ linearquadratic modelj 'Ï~ áf Ff y = 0.1016D + 0.0118D + 0.0147(y = CB ^ MN~ >, D = OÒ F Òï Gy)& . Þêö* "æ" &æ ÒG ²~ ² Bf 1,000B~ ^îªNâ* '' ï 8.90B 5 9.60B &b. &ç ²~ Î BÚöB ^ 2 B ç~ ²j &ê ãÖº ìî (Table 4). Ò &ç ²~ ² Bnê¢ þ' OÒF Ò ê á f OÒFï->wö &«~ º;Fïj 2k~V * ~, y = aD+bD +C¢ D = [ –a ± ( a – 4b ( C – y ) ) ]÷ 2b *~~ *~ j " ^ ²~ > ¢ &« : º;Fïf Fï->w êÂj * ² B. 2. 2. 2. Table 4. Micronucleus frequency in binucleated cells of bovine lymphocytes from Uljin nuclear power station region and control region Subject. Number of MN per 1, 000 CB cells Control region. Uljin region. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15. 10 4 7 7 21 10 10 13 7 7 9 8 11 8 12. 10 15 7 8 3 14 9 4 11 8. Mean±S.D.. 9.60±3.91. 8.90±3.84.

(8) B^¢Á;cÎÁBW^. 346. öB OÒF jÒ ~ BN Ô~b, ;B' º;Fïf Þêö* æ ÒG²öB -8.71 cGy, & æ ÒG²öB -5.05 cGy & .. V. OÒF b~ b' FïG;f '« Wî 5 Ö~ æz¢ 7b Î²æzG; ¯, z' O» [13, 34]" W?"ïÚw»(premature chromosome condensation) ¦Ò»j j ^F*' O» [18], .^ [7], ;¶Ö [24], ÚÎ~ æz [20]¢ Ï ^ç' O», Væ ' æz j &V~º O» B> ® . b' FïG;~ b , bïö V >w~ ¢~(dose-dependence)f *Ò OÒFö & ßû' >wj FïG; Ï ¢ ~ b ê Ö"~ ÖÂ, ¦ªb~ ¦Â " b ê bFï~ æ³W, òWb" ªb~ 'ÏW 5 · Fîö &~ Îv G; &Ë~¢ . ¾ *Ò *~ j Îv òÒ > ®º æº ìb¾ BÏ êGV¢ Ï bÒ' G;O »~ ^B6j j~V *~ b' Fï G;» 7º> ® [17]. OÒF bö & b' FïG; O»b ^ F*' ªC» ²¦Ò â*¢ " ÒÏ ~º, º jv' >« ^, ;ççöBº ª ~æ pb ~ j& Ï~V r^ [3, 10, 11, 22]. $ V~ "ïÚ ªC»ö j~ ²¦ Òº "ïÚ ¦Òö & ßê ?N¾ VF ìê ªC jv' Ö V*ö >¯F > ® . ß® ^îª N â*~ ÒÏö V¢ OÒFb ª¢~ & z× Ï~² >î [1, 9, 21, 32]. ²f *ÒOÒF~ ç7Î" $º free radicalsö ~ "ïÚ~ Z7.ª.(acentric fragment), vB ç ~ centromere Ò, kinetochore~ Ö¶ $º OºÒ~ ¶ç ö ~ ^ª "(main nucleus)ö >æ á ;W>º ©b rJ^ ® [1, 9, 21, 33]. ²¢ &çb *~ öB, ;ç& ~ ² >~& 1,000B ^îª N â* , 16-19B [8], 24.6B [23], 10-31B [28] >î . öB ²F¾ â* ² B Ö"¢ * ¶. ~ W'" jv~, öB Fï->wj êÂ ~V * 'ÏB ²~ â* >~ ï 15Bf F Ò~&b¾ &æ ÒG ²~ ï>~º £* Ô² ¾æÒ . º Fï->w êÂj * 'ÏB ²f &æ ²~ ÒG~ã~ N& &ê>º ©b '>, çV ¶~ öB 'ÏB ²~ _, ®. « 5 ÒG~ã ~ º &>Ò¢ 'B . º Þêö* "æ ÒG ²~ â* ² Bj æ OÒF b &ËW 6êj * ^ F*' B, ö* "æ ÒG ²f & æ ÒG ²öB jÝ . >~ 5 ² B >~¢ ¾æêb ö*ö ~ "æ ÒG ²~ OÒF b ' FWf ìº ©b ï&>î . &» F¾ ^ ¢ ÒÏ öBº &ç &»~ _ 7º~ [19] ß® ~ã' Fº~ ï&öB F¶~ &³ê ËV Â ;{ Ö"¢ ¾æÚÒ¢ Òò>, V¢B OÒW bî~ Ú¦ J"¾ *ÒOÒF~ ¦ b FZ 6j *~ ö¶KJ "æ~ &»ö &Bê _ &»j &çb & jº~ ÒòB . jÿb¾ &»f *~ ~ãj F~æ ç7 Ú¢ &çb ~º Ò¢ &Ú > ®º ÿb j &çb Ò& ê³>Ú¢ ©b ' > f ?f &6öB ö¶K J æ~ b6 Úê~ {ã jº~ .. Ö . Þêö¶KB*² "æ" &æ(*Î zB)öB Ò GB ²~ ö..j Ï~ .' ªC 5 ^ F*' ªCj ~& . ~ãOÒF Âö & æB ' æ ²~ â*¢ &çb ^îª N^¢ Fê~ ^öB ² G;j. ~& . þ&ÚöB þ'b OÒFj Ò â *¢ &çb êÂB ' Fï->wö ' æ ² öB G;B ² nê¢ 'Ï~ º; Fïj 2k ~& . Þêö* "æ ÒG ²f &æ ÒG ²~ ² Bf '' 1, 000B~ ^îª N â* 8.90B 5 9.60B & . ö* "æ ÒG ²f & æ ÒG ²~ .>~ 5 ²~ BöB F~ W ®º N& ìÚ, öB~ Ö"º Þêö¶K B*²f &æ~ OÒF &N FWf N& ì º ©b 'B .. ^^ò 1. Almassy, Z., Krepinsky, A. B., Bianci, A. and Koteles, G. J. The present state and perspectives of micronucleus assay in radiation protection. A review. Appl. Radiat. Isot. 1987, 38, 241-249. 2. Backer, L. C., Grindem, C. B., Corbett, W. T., Cullins, L. and Hunter, J. L. Pet dogs as sentinels for.

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