Effects of Acupuncture on Autonomic Nervous System in Normal Subjects under Mental Stress

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Effects of Acupuncture on Autonomic Nervous System in Normal Subjects under Mental Stress

Seong-Uk Park, Woo-Sang Jung, Sang-Kwan Moon, Jung-mi Park, Chang-Nam Ko, Ki-Ho Cho, Young-Suk Kim, Hyung-Sup Bae

Department of Cardiovascular & Neurologic Diseases (Stroke Center), College of Oriental Medicine, Kyung-Hee University, Seoul, Korea.

Objectives: The objective of this study was to assess the effects of acupuncture applied at PC6 and SP4 points on heart rate variability (HRV) in normal subjects under mental stress

Methods: 24 healthy male subjects were recruited and randomized to a treatment group (12 subjects) or a control group (12 subjects). Both groups performed a mental arithmetic stress test and then acupuncture needles were inserted on PC6 and SP4 for the study group. In the control group, they took sham acupuncture as a control. Heart rate variability (HRV) was measured during the whole study period by FM-150 (digital Holter ECG recorder).

Results: In the both groups, low frequency (LF) power and ratio of low frequency to high frequency (LF/HF) was significantly increased after mental stress. Acupuncture induced significant decrease in low frequency (LF) power and ratio of low frequency to high frequency (LF/HF) which were increased after mental stress test. But sham acupuncture induced no changes.

Results: In both groups, low frequency (LF) power and ratio of low frequency to high frequency (LF/HF) significantly increased after mental stress. Acupuncture induced significant decrease in low frequency (LF) power and ratio of low frequency to high frequency (LF/HF) which increased after mental stress test. In contrast, sham acupuncture induced no changes.

Conclusions: Acupuncture on PC6 and SP4 could be useful to decrease sympathetic activity and balance autonomic nervous system for those who are under stress.

Key Words : Heart rate variability (HRV), acupuncture, autonomic nervous system, mental stress

2008 3 10 2008 5 11

: , 149

(Tel:+82-2-440-6217, Fax:+82-2-440-6296, E-mail:[email protected])

2006 (KHU-20060435)

內關 公孫

박성욱, 정우상, 문상관, 박정미, 고창남, 조기호, 김영석, 배형섭

Original Article

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연구방법 1.

연구대상

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정신적 스트레스 유발시험

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심박변이도

측정

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, Digital Holter ECG recorder FM-150(Fukuda Denshi Co.,Ltd, Japan)

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. 24 Holter ECG recorder HRV

HRV . ECG recording

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(Pre-Stress Period),

5 (Post-Stress Period), 10

(Post-Acupuncture Period),

ECG recording HRV

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취혈방법

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(Palmaris Longus) .

2) (SP4): (metatarsal bone)

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5.

연구과정 24

12

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(Pre-Stress Period),

5 . 5

(Post-Stress Period) (PC6), (SP4) 2-3cm

15 . 10

(Post-Acupuncture Period).

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1cm 0.5cm

15 (Fig. 1). Circadian fluctuation

8 30

12

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5.

데이터 처리

1)

Holter Holter

software SCM-510(Fukuda Denshi Co.,Ltd, Japan) .

2)

SPSS 13.0 for windows

. baseline characteristics

Mann-

Whitney U test .

Wilcoxon signed-rank

test ,

Mann-Whitney U test .

p-value 0.05 .

연구결과

1.

피험자들의 일반적 특징과 안정 상태에서의

HRV

비교

24 12 12

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. HRV LF, HF, LF/HF ratio

(Table 1).

Acupuncture in Acupuncture out

0 min 10 min 15 min 20 min 35 min 45 min

HRV

Rest

Pre-stress Stress Rest

Post-stress

Acupuncture or Sham Acupuncture

Rest Post-Acupuncture

Fig. 1.

2.

시험군의

변화

LF LF/HF

ratio . -

LF LF/HF ratio . HF HR

(Table 2).

3.

대조군의

변화

LF LF/HF

ratio . Sham acup-

Study Group Control Group Sig

Age 21.30 ± 1.75 22.83 ± 2.75 N·S

HR 70.45 ± 5.60 68.43 ± 7.12 N·S

LF 718.90 ± 453.18 709.80 ± 342.16 N·S

HF 454.09 ± 116.48 424.97 ± 101.01 N·S

LF/HF 1.58 ± 0.67 1.67 ± 0.58 N·S

Values are presented as mean±SD(standard deviation).

* : tested by Mann-Whitney U test (p<0.05) sig: significance, N·S: no significance HRV: Heart rate variability, HR: heart rate

LF: low frequency, HF: high frequency, LF/HF: LF/HF ratio Table 1.

Pre-stress Post-stress Post-acupuncture

HR 70.45 ± 5.60 68.54 ± 6.20 68.79 ± 5.54

LF 718.90 ± 453.18 1708.07 ± 473.20

774.41 ± 464.10

HF 454.09 ± 116.48 484.12 ± 147.42 517.79 ± 121.03

LF/HF 1.58 ± 0.67 3.53 ± 0.65

1.50 ± 0.45

Values are presented as mean±SD(standard deviation).

* : Significantly different from Baseline by Wilcoxon signed-rank test

#: Significantly different from After-mental-stress period by Wilcoxon signed-rank test HRV: Heart rate variability, HR: heart rate

LF: low frequency, HF: high frequency, LF/HF: LF/HF ratio Table 2.

Pre-stress Post-stress Post-acupuncture

HR 68.43 ± 7.12 67.67 ± 6.30 64.03 ± 7.55

LF 709.80 ± 342.16 1764.49 ± 483.21

1539.72 ± 631.54

HF 424.97 ± 101.01 459.55 ± 180.18 525.98 ± 222.04

LF/HF 1.67 ± 0.57 3.84 ± 0.49

2.93 ± 0.87

Values are presented as mean±SD(standard deviation).

* : Significantly different from Baseline by Wilcoxon signed-rank test

#: Significantly different from After-mental-stress period by Wilcoxon signed-rank test

Table 3.

uncture LF LF/HF ratio (Table 3).

4.

시험군과 대조군의

변화값 비교

HRV ,

. LF LF/HF ratio

(Table 4).

고찰 및 결론

(heart rate variability, HRV)

. HRV low frequency (LF: 0.04 0.15 Hz), high frequency(HF: 0.15 0.40 Hz), LF/HF

ratio (Task Force of the

European Society of Cardiology and the North American Society of Pacing and Electrophys- iology 1996), LF (sinus node)

, HF ( )

, LF/HF ratio -

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reflex-induced, reversible Changes after stress Changes after acupuncture

Control Group Study Group Control Group Study Group

HR -0.76 ± 6.35 -1.91 ± 5.77 -3.64 ± 6.67 0.25 ± 5.96

LF 1054.69 ± 426.87 989.17 ± 432.16 -224.77 ± 532.17 -933.66 ± 425.81*

HF 34.58 ± 123.65 30.03 ± 109.63 66.43 ± 190.36 33.67 ± 116.25

LF/HF 2.17 ± 0.53 1.95 ± 0.63 -0.91 ± 0.64 -2.03 ± 0.55*

Values are presented as mean±SD(standard deviation).

* : tested by Mann-Whitney U test (p<0.05) HRV: Heart rate variability, HR: heart rate

LF: low frequency, HF: high frequency, LF/HF: LF/HF ratio

Table 4.

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(LI 4)

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(PC 6) (LI 4)

muscle sympathetic nerve activity (MSNA)

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