305 -P3-70
Ailanthus altissima induces apoptosis by activating p53 in Human lung cancer cell Department of Agrofood Resources, National Academy of Agricultural Science, RDA
Yu-Jin Hwang, Kang-Jin Cho, Haeng Ran Kim and Kyung-A Hwang* 가죽나무의 종양억제유전자 p53 활성화에 의한 인간 폐암 세포 아폽토시스 유도
국립농업과학원 농식품자원부 : 황유진, 조강진, 김행란, 황경아*
Objectives
The present study was investigated to identify the possible biochemical pathways involved, and assess the therapeutic potential of Ailanthus altissima in cancer. Cancer is still a leading cause of death worldwide, in spite of the intensive efforts and substantial advances that have occurred through focusing on improving treatments. The WHO indicates that one-third of all cancer deaths are preventable and that diet is closely linked to cancer prevention. On the basis of this idea, attention has centered on dietary phytochemicals as an effective intervention in cancer development. This study was performed to confirm the anticancer mechanism of various fractions of Ailanthus altissima on A549 human lung cancer cell cells.
Materials & Methods
Sample Preparation : The ethanol extract from Ailanthus altissima was fractionated into Hexane, n-BuOH, Chloroform and H2O. Cell Cytotoxicity : The cell cytotoxicity
were measured by MTT assay. Real-time PCR : Real-time PCR was performed using 2X SYBR Green. All results were normalized to GAPDH expression. DNA fragmentation: The DNA isolation was carried out using AllPrep DNA kit. Isolated DNA was electrophoresed in 2% agarose gels and visualized with gel Doc imaging system. Results
The present results provided evidence for the potential use of Ailanthus altissima in the prevention of human lung cancers. The results showed that Ailanthus altissim
significantly inhibited the proliferation of A549 cells, confirmed by the cell viability. The cytotoxic activity of Ailanthus altissima is likely to be due to the induction of apoptosis, as demonstrated by the DNA fragmentation results. To address apoptotic cell death A549 cell by Ailanthus altissima fractions, we investigated the expression of p53, p21, Bax and Bcl2. Treating with Ailanthus altissima led to induced apoptosis of A549 cells by down-regulating Bcl-2 gene expression and up-regulating p53, p21 and Bax expression. From these results, we expect that Ailanthus altissima extracts can be used nutraceutical and pharmaceutical materials for anti-cancer effect.
306 -* 시험성적
Fig. 1. The growth inhibition of Ailanthus altissima fractions on A549 cells.
Fig. 2. Induction of apoptosis by Ailanthus altissima fractions in A549 cells.
Fig. 3. mRNA expression of p53(a), p21(b), Bax(c) and Bcl2(d) in A549 cells treated with Ailanthus altissima fractions.
