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Effects of Immersion Liquid and High Hydrostatic Pressure on the Physicochemical Quality Characteristics of Scomber japonicus

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침지액 및 초정수압의 병행처리에 의한 고등어(Scomber japonicus) 의 물리화학적 품질 특성 변화

박지혜1, 배난영1, 박선희1, 김민지1, 김꽃봉우리2, 최정수3, 안동현1*

1부경대학교식품공학과/식품연구소

2부경대학교수산과학연구소

3경남정보대학교호텔외식조리계열

Received: December 4, 2015 / Revised: March 15, 2016 / Accepted: March 16, 2016

서 론

고등어(Scomber japonicus)농어목고등어과고등어속 속하는난류성회유어종으로우리나라에서어획량이 생선하나이다. 우리나라연근해와일본, 중국 미국캘리포니아연안등의태평양에주로분포하고 있으며우리나라에 8 17, 세계에 15 49종이분포하고 있다[22]. 정어리, 전갱이꽁치와함께 4등푸른생선으 불리며영양성분으로 docosahexaenoic acid (DHA, 22:5n-3)

eicosapentaenoic acid (EPA, 20:5n-3) 등과같은 ω-3 방산인 고도불포화지방산(polyunsaturated fatty acids,

PUFA)비율이 36.1%풍부하여영양적가치가높은

대표적인고지방어류이다[7].

그러나고등어는지질함량이매우높은적색육어류로 비단백질소성분이많이있어생선이부패하는동안 세균에의해이용되기때문에다른고단백식품보다변패가 쉬운것으로간주되고있다. 또한선도저하가매우빠른 종으로가공지질산화에의해빠르게산패되어불쾌취 유발하고단백질변성촉진영양가의저하품질에 나쁜영향을줌으로써가공제품이나요리로이용

한요인이되고있는실정이다[9, 20]. 이와같은이유로고등

어는염장품으로많이이용되고있으며[25], 최근들어이와 같은문제점들을개선하고고등어의이용을증대하기위하 Effects of Immersion Liquid and High Hydrostatic Pressure on the Physicochemical Quality Characteristics of Scomber japonicus

Ji-Hye Park1, Nan-Young Bae1, Sun-Hee Park1, Min-Ji Kim1, Koth-Bong-Woo-Ri Kim2, Jung-Su Choi3, and Dong-Hyun Ahn1*

1Department of Food Science & Technology/Institute of Food Science, Pukyong National University, Busan 48513, Republic of Korea

2Institute of Fisheries Sciences, Pukyong National University, Busan 46041, Republic of Korea

3Subdivision of Culinary Arts, Kyungnam College of Information and Technology, Busan 47011, Republic of Korea

This study investigated the effects of combined treatments of immersion liquid and high hydrostatic pres- sure (HHP, 100−400 MPa) on the quality of mackerel. In this study, we measured viable cell counts, pH level, color value, texture properties, and sensory evaluation of mackerel. Viable cell counts of mackerel treated with combined HHP were decreased by 2 log cycles at 300 MPa and 400 MPa compared to the control. The pH values of mackerel treated with HHP at 300 MPa and 400 MPa were higher than that of other samples.

Hardness, gumminess, and chewiness decreased when treated with combined HHP and increased with increase in HHP. Lightness and whiteness of mackerel increased, but redness decreased, with increase in HHP. In case of sensory evaluation, texture of mackerel treated with HHP at 300 MPa and 400 MPa showed higher scores than that of other samples. These results suggest that immersion liquid and HHP treatments can increase microbiological safety and improve textural properties of mackerel.

Keywords: Scomber japonicus, high hydrostatic pressure, texture, sensory evaluation

*Corresponding author

Tel: +82-51-629-5831, Fax: +82-51-629-5824 E-mail: [email protected]

© 2016, The Korean Society for Microbiology and Biotechnology

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고등어의저장성품질개선을위한연구가활발히

행되고있다. 예로고등어에자외선조사[27], 방사선

[5], 저온삼투압탈수법[18], 초정수압처리법[14] 등의 가열처리방법이진행되어있으며, 천연항산화제로써양파 생강즙[19], 유자액[11], 커피박[26], 한약재해조류[12]

등을사용하는연구들이보고되고있다.

한편초정수압처리는최근각광받고있는비가열처리 하나로압력이온도처럼열역학적인인자로작용하여 열처리와동일한살균효과를기대할있으면서소수성 이온결합에만영향을미치기때문에식품의고유풍미 영양성분을그대로유지할있는장점이있으며미생물 효소의활성을효과적으로제어할있다[21]. 초정수압 처리는열처리에의한변성기작과는달리분자의수화에 기인한단백질변성을일으킨다. 이는압력수준의증가에 단백질외부의분자들이단백질내부로침투되어단백 내부 공간 확장에 의하여 단백질구조가 일부 풀리는 molten globule 현상을야기하는것으로알려져있다[17].

연구진은이전연구에서침지액에담그지않고저장기 동안초정수압처리고등어의 histamine 저감화효과[14]

유자/매실침지액과초정수압(450 MPa) 병행처리에

냉장저장저장성증진효과[15]대해확인하였으 , 연구에서는침지액처리와함께다양한초정수압 조건에서(100400 MPa) 생균수, 물성, 색도, pH 능적특성변화를측정하여저장성향상뿐만아니라품질을 향상시킬있는최적의초정수압처리조건을제시하고자 한다.

재료 및 방법

실험재료

연구에사용된고등어는부산시수영구민락동소재의 현대수산에서활어상태로구입하여, 즉시머리와내장을 거하여평균두께 1.5 cm 2개의필렛형태로제조하였다. 침지액 제조 및 초정수압 처리

침지액은소금 0.8%, 설탕 2%조성으로제조하였으며,

진공포장지에고등어필렛을넣은, 동량무게의침지액을 첨가하고 진공 포장한 다음 초정수압기(215L-600Ultra, Avure Technologies Inc., OH, USA) processing chamber 넣어수온 1824oC에서각각 100, 200, 300, 400 MPa 3분간초정수압을처리하였다.

생균수 측정

초정수압처리한고등어육을무균적으로 2 g 취한 균된 PBS (Phosphate buffered saline, pH 7.4)첨가하여

10 희석한 homogenizer (AM-7, Ace homogenizer, Japan)사용하여 1000 rpm에서 1분간균질화한 10 희석법으로희석하였다. 희석된시료액을 PCA (plate count agar, BD DifcoTM, CA, USA)도말하여 37oC에서 24시간

배양한생성된집락을계수하여 CFU/g으로나타내었다.

pH 측정

고등어육을세절하여 5 g취한 10배량의증류수 50 ml 가하여 균질기(Ace Homogenizer, AM-7, Japan) 10,000 rpm에서 2분간 균질화한 , 실온에서 pH meter (HM-30V, TOA, Japan)이용하여측정하였으며 2반복 하였다.

물성 측정

시료를 2 × 2 × 1.5 cm자른경도(hardness), 부서짐 (fracturability), 부착성(adhesiveness), 탄력성(springiness), 응집성(cohesiveness), 검성(gumminess), 씹힘성(chewiness) 복원성(resilience)측정하였다. 이때 force 100 g, distance 10 mm, test speed 2.0 mm/s 조건에서 texture meter (T1-AT2, SMS Co., Japan)이용하여측정하였으며, 5 이상반복측정한평균값을취하였다.

색도 측정

시료를 1.5 × 1.5 cm 자른 색차계(JC801, Color Technosystem Co., Japan)측정하며, 고체 calibration시행 하여 standard 설정하였다(X = 94.98, Y = 96.78, Z = 115.38). 각각의 색도는 L* (lightness, 명도), a* (redness, 적색도), b* (yellowness, 황색도) 값을이용하여 측정하였 . 백색도(whiteness)백색도지표인 L*3b*이용하 계산하였다(L* = 93.73, a* = -0.12, b* = 0.11).

관능평가

평가를위해숙련된패널들(부경대학교식품공학과식품 자원개발실원 10)대상으로선정하여논의과정을통해 검사에필요한용어를마련하고, 용어에대한혼동을 소한으로하기위해용어에맞는정의를내리도록하였다. 준비된시료를일정한크기로잘라같은접시에놓아둔 7점수법을이용하여(fresh aroma), (fresh taste), (salinity), 경도(hardness), 탄력성(springiness) 5가지 항목을평가하였다. 점수는좌로부터우로이동하면서특성 강도가증가하도록선의양쪽에용어한계를표시하여 사하였다.

통계 분석

실험결과에 대한 통계 분석은 SAS program

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(Statistical analytical system V8.2, SAS Institute Inc.,

NC, USA)이용하여분산분석을하였으며처리구간의

유의성을검증하기위해 Duncan다중검정법을사용하여

평균값간의유의차를분석하였다(p < 0.05).

결과 및 고찰

미생물 생육 억제 효과

고등어필렛의침지액초정수압처리에의한미생물

억제효과를알아보기위해생균수측정을결과(Table

1), 침지액초정수압처리를하지않은무처리구에서는 2.75 × 105 CFU/g균수를보였으며초정수압처리를하지 않고침지액처리만처리구에서는 5.06 × 104 CFU/g 수를보여아무것도처리하지않았을때보다침지액처리를 했을균수가감소하는것을확인할있었다. 일반적으 염도가높을수록미생물생육이제어되는것으로알려져

있어염에의해균수가감소한것으로사료된다[13]. 침지액

처리를하여각각 100, 200, 300 400 MPa압력으로 정수압처리한처리구에서는 1.92 × 104, 1.37 × 104, 5.52 × 103 1.37 × 103 CFU/g균수를보여초정수압처리 력이증가함에따라균의생육이억제됨을확인하였다. 이는 Gou [8]보고에서조미오징어에 400 MPa초정수압 처리한미생물의수가대조군에비교하여저장초기 2.77 log cycle감소를보인결과와유사하며, 또한 Park

[23]생굴에 350 MPa 초정수압을처리한초기총세균

수가 101 CFU/ml초정수압처리에의해 1 log cycle

감소하였고저장 7일차에 103 CFU/ml저장기간동안 균수의증가가억제됨을확인한있다. 또한이는초정수 처리로미생물의세포막붕괴세포막에존재하는 백질의변성이일어나정상적인생육이어렵게되어미생물 수가감소된다는 Kang [14] Bull [2]연구와도일치 하였다.

pH 변화

고등어필렛의침지액초정수압처리가 pH미치는 효과를알아본결과(Table 2), 무처리구는 5.88, 침지액

리만처리구는 5.80이었으며, 침지액초정수압처리

처리구는각각 100, 200, 300 400 MPa압력에 따라 5.91, 5.81, 6.15 6.50으로 200 MPa압력까지는 무처리와비교하여유의적으로차이가적었으며 300 400 MPa압력이올라감에따라 pH유의적으로증가하는 경향을보였다. 이는일반적으로초정수압처리는단백질의 구조적변화를일으키며, 이에따라일부염기그룹의노출 야기함으로써 pH다소 증가시키는것으로보고되고 있다[16]. 또한 Hong [10]돈육에초정수압열처리한 초정수압처리에의해수분이단백질구조사이로침투 하여단백질풀림현상이일어남에따라단백질구조가 화하여 pH약간증가한다고보고하여연구결과와 치하였다.

물성

고등어필렛의침지액초정수압처리에따른물성의

화를 알아본 결과(Table 3), 경도의 경우 무처리구는

2175.65, 침지액처리만처리구는 3116.07침지액 리한처리구가무처리보다높았으며, 침지액초정수압 처리한처리구는각각 100, 200, 300 400 MPa압력에 따라 1138.11, 1314.81, 1833.82 2029.93으로압력이 가할수록경도가유의적으로 증가하는경향을보였다. Ju [13]연구에서염도가증가할수록고등어의경도가증가하 였다고하여연구결과에서침지액처리에의한경도의 증가는 NaCl의한것으로사료된다. Kim [16]연구 에서고등어연제품제조시설탕비율을 03%달리하였 을때, 12% 처리구에서는증가하였고, 3% 처리구에서는 소하는경향을보였고 0% 처리구보다는증가하는경향을 Table 1. Viable cell counts of mackerel muscle treated with

high hydrostatic pressure in immersion liquid.

Viable cell counts (CFU/g)

Untreated 2.75 × 105

Treated with immersion liquid1)

0 MPa 5.06 × 104

100 MPa 1.92 × 104 200 MPa 1.37 × 104 300 MPa 5.52 × 103 400 MPa 1.37 × 103

1)Immersion liquid with 0.8% NaCl and 2% sugar.

Table 2. pH value of mackerel muscle treated with high hydrostatic pressure in immersion liquid.

Untreated Treated with immersion liquid1)

0 MPa 100 MPa 200 MPa 300 MPa 400 MPa

5.88± 0.01c2) 5.80± 0.01d 5.91± 0.01c 5.81± 0.01d 6.15± 0.01b 6.50± 0.01a

1)Immersion liquid with 0.8% NaCl and 2% sugar.

2)Means with different superscripts in the same row (a−d) are significantly different (p < 0.05).

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였다. 따라서, 설탕이고등어연제품의경도, 검성, 씸힘성이 증가에영향을주는것으로나타났다. 따라서, 고등어에 용된설탕과소금의혼합작용에의해근육단백질의변화 나타난것으로사료된다. Chéret [4]연구에서는

어에초고압을 100 MPa처리시경도가감소하였다가 300

MPa 400 MPa에서경도가증가하는경향을보여연구

유사한경향을보였다. 200 MPa 이상의초고압처리시,

무처리구와비교하여근육이조밀해졌는데이는초고압에 의한단백질의겔화와관련이있는것으로보았으며, 압력이 증가할수록조밀도는증가하였다고하여연구에서의 경도의증가는이와관련이있는것으로사료된다.

또한검성의경우침지액초정수압처리압력이 100,

200, 300 400 MPa증가함에따라각각 238.2, 209.56,

280.35 438.91압력이증가할수록검성이유의적으로

증가하였으며씹힘성의경우에서도압력이증가함에따라 57.45, 45.19, 76.66 94.75값을나타내어압력이증가 할수록씹힘성이증가됨을확인할있었다. 검성의경우 경도와, 씹힘성은검성과정의상관관계를가지며, Chéret [4]연구에서도경도, 검성, 씹힘성은 300 MPa 이상에 압력이증가함에따라증가하는것으로나타났다. 부서짐 , 부착성, 탄력성응집성의경우초고압처리전후 렷한변화를보이지않았으며, 복원성의경우침지액처리구

무처리구에비해감소하였고, 초고압처리이후 0 MPa 처리구에비해감소하였으나압력증가에따른유의적인 이는나타나지않았다. 따라서, 고등어침지처리구에초고 처리시압력이증가함에따라경도, 검성, 씸힘성이증가 함을확인하였고, 관능평가결과에서도유사한경향을나타 내었으며, 물성의변화에따른신선한평가에서는초고압 처리전후차이를나타내지않아초고압처리가고등어 질감의변화에긍정적인효과를나타낸것으로사료된다. 색도

고등어필렛의침지액초정수압처리에따른색도 화를알아본결과(Table 4), 명도의경우무처리구는 45.81, 침지액처리만처리구에서는 46.60이었으며침지액 정수압처리한처리구는각각 100, 200, 300 400 MPa 압력에따라 49.71, 60.83, 69.40 73.30값을나타내어 초정수압의압력이증가할수록명도는유의적으로증가함을 확인하였다. 황색도의경우무처리구는 1.29, 침지액처리만 처리구에서는 -0.57이었으며침지액초정수압처리 처리구는각각 100, 200, 300 400 MPa압력에 -0.82, 0.66, -3.81 -1.61값을나타내어황색도는 의적으로감소하는경향을보였으며적색도의경우에는 200 MPa 처리구가유의적으로가장높았으며, 나머지처리구에 Table 3. Texture profile analysis of mackerel muscle treated with high hydrostatic pressure in immersion liquid.

Untreated Treated with immersion liquid1)

0 MPa 100 MPa 200 MPa 300 MPa 400 MPa

Hardness 2175.65± 26.74b2) 3116.07± 63.86a 1138.11± 2.96f 1314.81± 30.75e 1833.82± 22.91d 2029.93± 39.08c Fracturability 02.85± 0.08a 01.33± 0.24b 02.53± 0.17ab -0.19± 0.07c 02.36± 0.77ab 03.05± 0.03a Adhesiveness -8.61± 0.31a -12.88± 0.14ab -25.75± 2.86d -13.24± 1.12b -33.22± 0.47e -18.61± 0.01c Springiness 00.31± 0.00c 00.32± 0.00b 00.25± 0.00d 0.25± 0.00d 0.28± 0.07a 0.24± 0.00e Cohesiveness 00.20± 0.00b 00.25± 0.00a 00.19± 0.00c 0.19± 0.00c 0.18± 0.00d 0.20± 0.00b Gumminess 459.27± 12.98b 783.02± 14.21a 238.2± 3.15c 209.56± 8.85c 280.35± 47.49c 438.91± 34.66b Chewiness 159.05± 3.33b 0285.8± 1.32a 57.45± 0.51e 45.19± 0.15f 76.66± 0.91d 94.75± 6.44c Resilience 00.86± 0.05a 00.26± 0.01b 00.06± 0.00c 0.05± 0.00c 0.06± 0.00c 0.06± 0.00c

1)Immersion liquid with 0.8% NaCl and 2% sugar.

2)Means with different superscripts in the same row (a−f) are significantly different (p < 0.05).

Table 4. Color value of mackerel muscle treated with high hydrostatic pressure in immersion liquid.

Untreated Treated with immersion liquid1)

0 MPa 100 MPa 200 MPa 300 MPa 400 MPa

Lightness 45.81± 0.16e2) 46.60± 0.32e 49.71± 0.31d 60.83± 0.14c 69.40± 0.39b 73.30± 0.07a Redness -0.36± 0.35b -0.58± 0.29b -0.68± 0.05b 0.46± 0.28a -0.68± 0.04b -0.90± 0.04b Yellowness 1.29± 0.01a -0.57± 0.04c -0.82± 0.32c 0.66± 0.18b -3.81± 0.01e -1.61± 0.02d Whiteness (L-3b) 41.94± 0.18f 48.31± 0.28e 52.17± 0.92d 58.85± 0.57c 80.83± 0.51a 78.13± 0.01b

1)Immersion liquid with 0.8% NaCl and 2% sugar.

2)Means with different superscripts in the same row (a−f) are significantly different (p < 0.05).

(5)

서는유의적인차이가없었으나, 초고압처리에의해적색도 감소하는경향을나타내었다. 백색도의경우무처리구는

41.94, 침지액처리만처리구에서는 48.31이었으며침지

초정수압 처리한 처리구는 각각 100, 200, 300

400 MPa압력에따라 52.17, 58.85, 80.83 78.13 나타내어초정수압의압력이증가할수록백색도가유의 적으로증가함을확인하였다. 결과적으로이와같은결과는 초정수압처리에따른단백질변성으로색의변화가일어난 것으로사료되며 Cruz-Romero [6]굴에 100800 MPa 초정수압처리를하였을명도가증가하고적색도가 감소하는것이근원섬유와근소포단백질의변성과관련 다고보고하였다. 또한날개다랑어[24] 대서양연어[28]

에서도명도가증가하고, 적색도황색도가감소하는결과 보였다. 이러한 변화는 globin 변성 또는 heme 변위 (displacement) 또는방출(release)기인한것으로사료된 [3].

관능평가

고등어필렛의침지액초정수압처리가관능적특성에 미치는효과를알아보기위해, , 염도, 경도, 탄력성 목에대해정량적묘사분석을실시한결과(Table 5), 신선한 향의경우무처리구에서는 3.7, 침지액처리만처리구에 서는 3.9점수를받았으며침지액초정수압처리를 처리구에서는 100, 200, 300 400 MPa압력에따라 3.6, 3.6, 3.7 3.5점수를받아유의적인차이를나타 내지않았다. 신선한맛의경우초정수압의압력이높아짐에 따라 3.6, 3.7, 3.6 3.6점수를받아유의적인차이가

었다. 염도의경우침지액처리만하였을때는 3.9, 침지액

초정수압처리를하였을 100 200 MPa압력에서 4.0 4.2 점수를 받아 염도가 증가했지만 300 400 MPa압력에서 3.9 3.5점수를받아약간감소하 경향을보였지만유의적인차이를보이지않았다. 경도의

경우침지액처리만하였을때는 3.0, 침지액초정수압

리한경우에서는압력이증가함에따라각각 2.4, 2.7, 3.6

4.2점수를받아전체적으로는압력이증가할수록경도가

높아지는경향을보였으며 100 MPa압력에서는침지액

처리만처리구보다경도가유의적으로낮았지만 300

400 MPa압력에서는침지액처리만처리구와비교하

다소높은경향을보였다. 탄력성의경우침지액처리만 처리구에서는 3.1, 초정수압처리한처리구에서는 2.4, 2.6, 3.4 3.6으로 100 MPa압력을처리하였을탄력성이 낮아졌다가 300 MPa 이상의압력에서침지액처리만 리구보다탄력성이유의적으로증가하여경도의결과와 치하였다. 이는초정수압처리로고등어단백질의변성으 인해경도가강해진것으로사료되며초정수압처리한 육의경우초정수압처리하지않은대조구에비해보수력이 향상된다고알려져있어[1] 연구와유사하였다.

요 약

연구에서는고등어필렛에침지액초정수압을처리 하여침지액과초정수압의압력에따른고등어필렛의품질

특성변화를알아보기위해생균수, pH, 물성, 색차관능

평가를실시하였다. 생균수를측정한결과, 침지액처리를 였을무처리구보다균수가감소하였고침지액초정수 압을병행처리하였을압력이높아질수록 2 log cycle 균수가감소하여생육이억제됨을확인하였다. pH 측정 결과, 초정수압처리에의해압력이증가할수록단백질의 조가변하면서 pH약간증가하는경향을나타내었다. 성의경우압력이증가할수록경도, 검성씹힘성이증가 하였으며다른항목들에서는유의적인차이가없는것으 나타났다. 색도의경우초정수압처리단백질의변성 으로압력이증가할수록명도와백색도가증가하였다. 관능 평가결과, 초정수압처리압력이높아질수록경도와 력성이증가함을보였다. 따라서연구결과, 고등어필렛 침지액초정수압을병행처리하였을미생물의 과적인증식억제로고등어의선도저하방지품질개선 효과적일것으로사료된다.

Table 5. Quantitative descriptive analysis of mackerel muscle treated with high hydrostatic pressure in immersion liquid.

Untreated Treated with immersion liquid1)

0 MPa 100 MPa 200 MPa 300 MPa 400 MPa

Fresh aroma 3.70± 1.16a2) 3.90± 0.74a 3.60± 0.84a 3.60± 0.70a 3.70± 0.67a 3.50± 0.85a Fresh taste 3.20± 1.03a 3.80± 1.14a 3.60± 0.84a 3.70± 0.67a 3.60± 1.17a 3.60± 1.26a Salinity 3.30± 1.06a 3.90± 0.88a 4.00± 0.94a 4.20± 1.03a 3.90± 0.99a 3.50± 0.97a Hardness 3.10± 1.20bc 3.00± 1.25bc 2.40± 0.70c 2.70± 0.82bc 3.60± 1.17ab 4.20± 1.32a Springiness 3.30± 1.06abc 3.10± 1.20abc 2.40± 0.97c 2.60± 0.70bc 3.40± 0.84ab 3.60± 0.97a

1) Immersion liquid with 0.8% NaCl and 2% sugar.

2)Means with different superscripts in the same row (a−c) are significantly different (p < 0.05).

(6)

Acknowledgments

This study was supported in part by a Basic Research Project through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (No.

2013R1A1A2009906).

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수치

Table 2. pH value of mackerel  muscle treated with high hydrostatic pressure in immersion liquid.
Table 4. Color value of mackerel  muscle treated with high hydrostatic pressure in immersion liquid.
Table 5. Quantitative descriptive analysis of mackerel  muscle treated with high hydrostatic pressure in immersion liquid.

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