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A pattern of cell death induced by 40 kHz ultrasound in yeast cell model

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Fig. 1. Irradiation system of 40 kHz ultrasound applied to the yeast cell culture of the 1.5 ml Eppendorf tube.
Fig.  2.  Ab sorb ance  at  wavelength  355  nm  of  KI  solution with different distilled water level in irradiation  system
Fig. 3. For cell viability test, overnight cultured yeast cells were treated with 40 kHz ultrasound (US) at various  times, incubated for 24 h (left), and then measured at absorbance of 660 nm with different time intervals (right)  for cell death detection
Fig. 5. Reactive oxygen species levels of yeast cells irradiated with 40 kHz ultrasound irradiation at various times were assessed by dichlorohydrouorescein diacetate staining under fluorescence microscope
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