Effects of Sweet Bee Venom on the respiratory system in Rats

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(1)ISSN 2093-6966. Journal of Pharmacopuncture. 47 http://dx.doi.org/10.3831/KPI.2011.14.3.047. Original Article. Sweet Bee Venom 시술이 Rat의 호흡기계에 미치는 영향. Effects of Sweet Bee Venom on the respiratory system in Rats. Received :May 31, 2011 Accepted :Aug 29, 2011. Jong Young Lee1, Ki Rok Kwon2*. KEY WORDS: Melittin; Sweet Bee Venom; whole body plethysmography; respiratory system. 2. 1 Sangji University Graduate School of Oriental Medicine, Wonju, Korea Research Center of Pharmacopuncture Medicine, Korean Pharmacopuncture Institute, Seoul, Korea. ABSTRACT Objectives: This study was performed to analyse the effects of Sweet Bee Venom(SBV-purified melittin supported by G&V Co., the major component of honey bee venom) on the respiratory system in rats. Methods: All experiments were conducted at Biotoxtech Company, a non-clinical studies authorized institution, under the regulations of Good Laboratory Practice(GLP). Male rats of 5 weeks old were chosen for this study and after confirming condition of rats was stable, SBV was administered in thigh muscle of rats in 0.175, 0.35 and 0.7 dosage. And checked the effects of SBV on the respiratory system using the whole body plethysmography. And respiratory rate, tidal volume and minute volume of rats were checked after administered SBV (melittin). Results: 1. In the measurement of respiratory rate, there were not observed any significant differences compared with control group. 2. In the measurement of tidal volume, there was not observed any significant differences compared with control group. 3. In the measurement of minute volume, 0.35 dosage group showed significant differences compared with control group. But we estimated that this result was caused by individual differences. Conclusions: Above findings suggest that SBV seems to be safe treatment in the respiratory system of rats. And further studies on the subject should be conducted to yield more concrete evidences.. I. 서론. cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.. 2011 Korean Pharmacopuncture Instltute. Corresponding Author Ki Rok Kwon. Research Center of Pharmacopuncture Medicine, Korean Pharmacopuncture Institute, Seoul, Korea Tel: +82-2-2658-9051 E-mail: [email protected] http://pharmacopuncture.co.kr.

(2) 48. Journal of Pharmacopuncture 제 14권 제 3호( 2011년 9월). II. 재료 및 방법 Apis melifera 1. SBV SBV Germany Saline. LA230S, CP323S, Sartorius, Normal vortex mixer 0.875, 1.75 3.5. SBV. ana-. 2. phylactic shock 2.1 Sprague-Dawley SD rat Sweet Bee Venom SBV melittin. 10-0744755. SBV. 106.7. Melittin 50 tide melittin. 40pep-. 26 2840 gel filtration. SBV. rat. 139.1g. 20.3. 5. 23.6. 40.5 58.5 Teklad Certified Irradiated Global 18 Protein Rodent Diet 2918C 2005-79 Good Laboratory Practice GLP. SBV 50. 100 2.2 27. SBV. 24 6. rat SBV body plethysmography system Respiratory rate 1 minute volume. 4. whole tidal volume. 2.3 1 SBV 60. 0.1. 0.175. 0.007 100 0.7 0.35 SBV. 0.4.

(3) 49. normal saline 0.2 2. 3. 3 Fig 1. The aspect of whole body plethysmography system The system of whole body plethysmography provide opportunities to assess upper airway breathing more objectively. Respiratory rate, tidal volume and minute volume of rats were checked after administered Sweet Bee Venom (melittin).. 3.1 1 chamber. 1 SBV 30. SBV chamber. 1 chamber. 4 3. 3.2. 4. parameters. 4.1 SBV 0 0.5, 1, 2. 4. 4.2 Unrestrained whole body plethysmography system, Buxco Electronics, Inc., U.S.A. chamber 1 parameters respiratory rate 1 tidal volume minute volume Biosystem XA Version 2.11, Buxco Electronics, Inc., U.S.A. 10 10 Fig. 1-2. Fig 2. The process how to performed respiratory functional test in rats 1. entered rats for experiment, 2. refined for a week in the cage, 3. checked respiratory function by whole body plethysmography, 4. observation and measurement and finally analyzed the data.. 4.3. 6. 3.

(4) Journal of Pharmacopuncture 제 14권 제 3호( 2011년 9월). 50. 6 6 3 4.4 SBV. parameters SBV Fig 3. Effects of Sweet BV(melittin) on respiratory rate in rats Values are mean S.D., N=6 **p 0.01, Significant difference from the control group (G1) by Dunnett s t-test. 5 1 SAS Ver. 9.1.3, SAS Institute Inc., U.S.A.. 3. 1. One-way analysis of variance ANOVA p 0.05. SBV al volume. 1 0.5, 1, 2. 0. Dunnett's t-test p 0.05, p 0.01. tid4. Fig. 4. III. 결과 1. 1. 1 SBV Fig 4. Effects of Sweet BV(melittin) on tidal volume in rats Values are mean S.D., N=6 No statistically significant differences were noted in the control group (G1) and the test substance group (G2 ~ G4) (p 0.05, ANOVA). 2. 0.35 piratory rate. SBV 4. resp 0.01. 0.175. 0.7 0. SBV 0.5, 1, 2. 4. 0.35 volume. minute p 0.01. 4 0.175. Fig. 3. SBV. 0.7 0. SBV 0.5, 1, 2. 4.

(5) 51. Fig. 5. 2840 allergen. melittin. Fig 5. Effects of Sweet BV(melittin) on minute volume in rats Values are mean S.D., N=6 **p 0.01, Significant difference from the control group (G1) by Dunnett s t-test. 26 peptide PLA2. 4. melittin nuclear factorB NF- B lipopolysaccharide LPS tumor necrosis factor- TNFsodium nitroprusside SNP SBV. IV. 고찰. SBV. poison, toxin, toxicant 0.1 Apis mellifera. 0.007 0.175, 0.35. 0.4. human. 0.7. SBV 60 SD rat SBV 50-100. 1 whole body plethysmography system 0.175 40. anaphylactic. 0.7. SBV. 1. 0.35. SBV. shock 1 SBV. PLA2. hyaluronidase. phospholipase A2 histamine. SBV 0.7. melittin Melittin. SBV 40-50.

(6) Journal of Pharmacopuncture 제 14권 제 3호( 2011년 9월). 52. 0.35. SBV. 4. SBV SBV 100 SBV. V. 결론 SBV rat SBV plethysmography system. 1.. whole body 1. 0.35. SBV. 4. 2. 1. 0 0.5, 1, 2. 3.. 4. 0.35. SBV. 4. SBV. VI. References 1. Academic committee of KPI. Pharmacopunct-. urology. Elsevier Korea. 2008:167-99. 2. Kwon KR, Koh HK. An Experimental Study with Bee Venom Therapy on Anti-inflammatory and Analgesic Effects. The Journal of Korean Acupuncture & Moxibustion Society. 1998;15(2): 97-103. 3. Ko HK. Experimental Studies on the Effect of Bee Venom Theraphy on the Analgesic, Antipyretic and Anti-inflammatory Action. Korean Journal of Oriental Medicine. 1992;13(1):283-292. 4. Kwon KR, Koh HK, Kim YS, Park YB, Kim CH, Kang SK. The Effect of Bee Venom Aqua - acupuncture on the Antitumor and Immune Response in the Epithelioma by 3 - MCA. The Journal of Korean Acupuncture & Moxibustion Society. 1997;14(2):151-172. 5. Lee SN, Hong SY, Jo HC, Byeon IJ, Song HS, Kim GH. The Clinical Study on Bee Venom Acupuncture Treatment on Osteoarthritis of Knee Joint. The Journal of Korean Acupuncture & Moxibustion Society. 2003;20(5):73-8. 6. Wang WH, Ahn KB, Lim JK, Jang HS. Clincal Investigation Compared with the Effects of the Bee Venom - Acupuncture on Knee Joint with Osteoarthritis. The Journal of Korean Acupuncture & Moxibustion Society. 2001;18(3):35-47. 7. Kim TH. Kang KS, Kwon KR. Three cases of affections of the hip treated with Korean BeeVenom therapy. Journal of Pharmacopuncture. 2001;4(3):122-130. 8. Lee SH, Lee HJ, Baek YH, Kim SY, Park JK, Hong SJ, Yang HI, Kim KS, Lee JD, Choi DY, Lee DI. Effects of Bee Venom on the pain, edema, and acute inflammatory reactant of Rheumatoid Arthritis patients. The Journal of Korean Acupuncture & Moxibustion Society. 2003;20(2):77-84. 9. Lee SH, Hong SJ, Kim SY, Yang HI, Lee JD, Choi DY, Lee DI, Lee YH. Randomized Controlled Double Blind Study of Bee Venom Ther-.

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