WCIM 2014 SEOUL KOREA 455
Poster Session
The Korean Journal of Internal Medicine Vol. 29, No. 5 (Suppl. 1)
with simvastatin signifi cantly reversed the decreased viability of H1975 cells and the increased expression of pro-apoptotic proteins.
Conclusions: Simvastatin restored the expression of BIM to induce mitochondrial apoptosis in cells harboring T790M mutation. Our study suggests the probability of simvastatin to overcome gefi tinib resistance with T790M mutation.
PS 1591 Lung Cancer
Malignant Bronchial Cytologic Result without Visible Endobronchial Lesions Can Predict Prognosis in Lung Cancer Patients?
Hwa Young Lee1, Hye Yon Lee1, Chin Kook Rhee1, Seung Joon Kim1, Seok Chan Kim1, Sook Young Lee1, Ji Young Kang1
Division of Respiratory, Allergy and Critical Care, The Catholic University of Korea, Seoul St. Mary`s Hos- pital, Korea1
Background: Bronchoscopy is a routinely performed procedure in the initial evaluation of lung cancer. However, malignant bronchial fl uid cytology from washing or brushing specimens as a prognostic factor has not been verifi ed clearly in lung cancer patients.
Methods: Of 310 patients who were diagnosed lung cancer and performed a broncho- sopy for initial staging work up between January 2009 and August 2010, 109 patients who had no endobronchial lesions were enrolled. Retrospective chart review was done to identify baseline clinical characteristics and prognosis.
Results: Among the 109 patients, 20 had positive malignant cells on bronchial brush- ing or washing fl uid cytology. Mean age was 66 years and 74 (67.9%) patients were male. Seventy-three (67%) patients were adenocardinoma, 22 (20.2%) squamous cell carcinoma, 10 small cell carcinoma and 2 adenosquamous cell carcinoma. Fourty-three (39.4%) patients were stage IV at initial diagnosis, 34 (31.2%) stage I, 26 (23.9%) stage III and 5 (5.5%) stage II. For mean 27 months’ follow up periods, mortality was 29.4% and mean progression free time was 10 months. Overall survival and progres- sion-free survival were higher in patients with malignant cytologic results than in pa- tients without it. But a multivariate analysis demonstrated that whereas cancer type and initial stage were signifi cant prognostic factors for overall survival, the result of malignant cells on washing or brushing fl uid cytology was not.
Conclusions: This study shows that malignant cytologic results from initial bronchial specimens could not be a signifi cant prognostic factor in lung cancer patients, al- though the initial staging was not similar between the two groups. Large scale further studies should be done to determine whether the initial cytologic results are associat- ed with overall survival and progression free survival in lung cancer patients.
PS 1592 Lung Cancer
Association Between Lung Cancer Risk and Putative Functional Variants of XRCC1 That Were Selected Using RegulomeDB in a Korean Population
Seung Soo Yoo1, Chengcheng Jin2, Jin Eun Choi2, Keum Ju Choi1, So Yeon Lee1, Yong Dae Lee1, Shin Yup Lee1, Jaehee Lee1, Seung Ick Cha1, Chang Ho Kim1, Jae Yong Park1 Internal Medicine, Kyungpook National University School of Medicine, Korea1, Biochemistry and Cell Biology, Kyungpook National University School of Medicine, Korea2
Background: ENCODE project revealed that nearby or distantly located non-coding DNA regulates the expression of coding genes. RegulomeDB is a new database that can be used to predict whether variants affect transcription factor binding and gene expression.
We investigated the association between lung cancer risk and potentially functional pol- ymorphisms of XRCC1 that were selected using RegulomeDB in a Korean population.
Methods: 185 polymorphisms of XRCC1 were evaluated using RegulomeDB. There was strong evidence that 10 polymorphisms affected XRCC1 expression with scores of 1a–1f that were based on the RegulomeDB. rs2854510 polymorphism was rare in Asians. Eight polymorphisms were in strong linkage disequilibrium (LD). rs2854509, which was one of the 8 polymorphisms in LD, and rs7248167, which was not in the LD block, were genotyped using the Taq-Man® assay in 610 lung cancer patients and 607 age- and sex-matched controls. Additionally, 4 polymorphisms of XRCC1 (Arg399Gln, Arg280His, Arg194Trp, and -77T>C), which were investigated with regard to the association with lung cancer risk in previous studies, were also genotyped.
Results: Two polymorphisms (rs2854509 and rs7248167) that were predicted to affect XRCC1 expression based on their RegulomeDB scores were not associated with lung cancer risk (P = 0.31 and 0.93, respectively). When stratifi ed according to age, gender, smoking status, and tumor histology, the 2 polymorphisms of XRCC1 were not associat- ed with lung cancer risk. Among the 4 polymorphisms that were previously studied, only XRCC1 Arg280His was signifi cantly associated with lung cancer risk (dominant model, adjusted odds ratio = 0.61, 95 % confi dence interval = 0.46–0.83, P = 0.002).
Conclusions: Although RegulomeDB is an attractive tool for predicting regulatory po- tential of variants, the 2 polymorphisms that were selected using RegulomeDB were not associated with lung cancer risk.
PS 1593 Lung Cancer
Polymorphisms in TERT Are Associated with Lung Cancer Risk in a Korean Population
Seung Soo Yoo1, Sook Kyung Do2, Jin Eun Choi2, Keum Ju Choi1, So Yeon Lee1, Yong Dae Lee1, Shin Yup Lee1, Jaehee Lee1, Seung Ick Cha1, Chang Ho Kim1, Jae Yong Park1 Internal Medicine, Kyungpook National University School of Medicine, Korea1, Biochemistry and Cell Biology, Kyungpook National University School of Medicine, Korea2
Background: Telomeres have an important role in the maintenance of chromosome integrity and stability. Short telomeres are associated with human aging, age-related diseases, and tumor development, including lung cancer. We investigated the associa- tion between lung cancer risk and polymorphisms that may affect telomere length.
Methods: Six polymorphisms of TERT, TERC or ZNF676 were genotyped using the Taq- Man® assay or polymerase chain reaction-restriction fragment length polymorphism assay in 1100 lung cancer patients and 1100 age- and sex-matched controls.
Results: Two polymorphisms of TERT were signifi cantly associated with lung cancer risk. The rs2736098 AA genotype was associated with an increased risk for lung cancer compared to that of the GG or GA genotype (adjusted odds ratio = 1.36, 95%
confi dence interval = 1.03–1.80, P = 0.03). TERT rs2853669 T>C was also associated with increased lung cancer risk (under recessive model, adjusted odds ratio = 1.37, 95% confi dence interval = 1.04–1.80, P = 0.02).
Conclusions: These fi ndings suggest that two polymorphisms (rs2736098 G>A and rs2853669 T>C) in TERT may contribute to genetic susceptibility to lung cancer.
