Seroprevalence of Mycoplasma hyopneumoniae infection in Korean swine herds

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(1)³¤¥Èó. * .* a. (2005) 45 1 Korean J Vet Res(2005) 45(1) : 55~61. ÖÒ¾¢·îËö*.ZDPQMBTNBIZPQOFVNPOJBFF÷N`Ò. BB²ÁB[Áª«9 Á·^ Á;/

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(3). *ÞL >~ zÒr zÒj() (²Òß: 2005j 2ú 28¢) 1. Seroprevalence of Mycoplasma hyopneumoniae infection in Korean swine herds Hye-kwon Kim, Tae-yung Kim, Jong-sung Lim1, Yang-ho Lee1, Bong-kyun Park* College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea 1 Merial Korea Ltd., Seoul 137-040, Korea (Accepted= February 28, 2005) Abstract : Serum samples of 1,175 pigs from 148 Korean swine farms not using Mycoplasma hyopneumoniae (M. hyo) vaccines were collected for seroepidemiological study of M. hyo infection by indirect ELISA method. Informations of each farm were provided about province where the farm was located and season when blood samples were collected. Then, the selected farms were divided into farm units which had 5 serum samples according to production stages : sow, suckling piglet (<30 days old), nursery pig (30-70 days old), and growing pig (>70 days old). Seroprevalence of M. hyo infection according to production stages, province, and season was investigated by using ELISA-positve rate of the selected samples for each study. This study showed that 85.34% (78.94-91.78%, 95% CI) of farms were positive to M. hyo infection and 34.81% (32.09-37.53%, 95% CI) among pigs were sero-positive to M. hyo infection in Korean swine farms. In the study of seroprevalence by production stage, most farms had sows and growing pigs which were sero-positive to M. hyo infection (sow: 83.05%, growing pigs: 87.72%) and most pigs seemed to be naturally infected by M. hyo at 8-10 weeks of age. Also, M. hyo infection showed seasonal pattern that most pigs were infected in late fall to early winter. However, in the study of seroprevalence by province, there was no significant correlation between province and M. hyo sero-positive rate. Key words : Mycoplasma hyopneumoniae, Seroprevalence, ELISA, Korean swine herds. * V. *2Oö &*º ;{® rJê :& ìb¾, M. 6"î ®º ÖÒ~ î`ö* 6" *þ 7V ç Ã& º & ® [10], ç77/ *2 ö ®Ú &Ë n® ·¢ '>Ú æ ® . ¾ V 7öBê M. hyoj ªÒbB [13], V*2 $ &Ë *2O~ ~¾& F > ®j © b 'B . &æ ~ãº 5 "æV^W VÃê:Ê(PRRSV, porcine reproductive and respiratory syndrome virus), porcine circovirus hyo. Mycoplasma hyopneumoniae(M. hyo)º "æö* î z2¢®îöZj FB~º 7º ¶* ]Ú &¦ ª~ ·îËö* ^B& >º î÷ [2]. ªçÃçf *ö' î 8 9Ë &~, ÒòÎN &~ . ç' ÷æf ^8 4Î~ F î£b * ÷ ê¯> ßû'b 8êf . "æö ª2 \& J>Ú germinal center¢ ;9 [1, 4, 6].. *º ³ êÓ Biogreen 21 “Z"æ~ î÷ Îî8ç 8F {ã” BÞL >~\²~ æöbR >¯>îr. *Corresponding author: Bong-kyun Park College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea [Tel: +82-2-880-1255, Fax: +82-2-885-0263, E-mail: [email protected]]. 55.

(4) BB²ÁB[Áª«9Á·^Á;/. 56. b~ 6"" îz2¢®îöZ"~ &Wö & $ ô Úr [2, 3, 10, 14]. ~æò, ÒG~ã~ ÓÖ ¦, PRRSV, porcine circovirus " M. hyo~ 6" ·çöº ` &W ì º ê ®. [14]. êf PCR, .Ó' ¦Ò j Û M. hyo 6" j ê > ®² >î . .Ó' ¦Ò~ ãÖ, Mycoplasma «"~ vN>w ^B>Ú zæò, 1989 jö M. hyo ßöj ªÒ êbB, vN>wj ²z ELISA& &Ër [7]. ÖÒ¾¢öBº 2004jö "æ~ "_" .Ó¦Ò · WN *~ ç&&ê¢ ªC Ö", 7-12"_ ;ê~ F¶î öVöB GWê 7Úº 6ö M. hyo ¶6" n®~² B~º ©b Ò>Úr [8]. ~æò, ÖÒ¾¢öB ' ÒGêö 8 ;{ F÷ N~ Nº Ò>æ p~, æê, ê.ê F÷N~ N $ B : ì . 8¢B, þöBº BÞ &v >~"& Öëÿb:Ê þ ~Ö> Úê .Ó þ2j Û ÖÒ¾¢öB "æ ÒGêê, æê, ê.ê M. hyo 6"·çö & ªC ¶ ~& .. Òò 5 O». . þ2 .þ2f 2003j 5ú¦V 2004j 12úræ ·î* ^ >~Òö ~ j.>Ú þ ~Ö>Úê © j &çb ~& . .j 2,500 rpmb 25ª* ö ªÒ~ .Ój ªÒ~&, ªÒB .Óf .Ó¦Ò Hræ -20 CöB ïÿ >î . .Ó ¦Ò ªÒB .ÓöB M. hyoö & Úº *7 ELISA B ¦Â~&b, IDEXX (Westbrook, USA)~ Mycoplasma hyopneumoniae antibody test kitf B¢: J(])~ Mycoplasma hyopneumoniae antibody test kit¢ ÒÏ~& . þO»f B®~ ÒÏJ«Bö 8¢ ¯r . 2003j 5úöB 2004j 5úræº IDEXX kit& ÒÏ>î, êú B¢:J kit& ÒÏ>î . IDEXX kit~ ãÖ 650 nm W%êöB OD 8j G; ê positive control negative control~ 8 ö 8¢ S/P ratio 8j \~& {S/P ratio = (Sample OD− Negative OD)/(Positive OD− Negative OD)}. S/P ratio 8 0.4 ç¢ ãÖ ·W, 0.3-0.4¢ ãÖ ~·Wb 6;~² >º, þöB ~·Wf ·Wb /~æ p~ . B¢:J kit~ ãÖº, 410 nm W% o. êöB OD 8j G;~&b, îR&æ S/P ratio& 0.4 ç¢ ãÖò ·Wb 6;~& . ·îË~ .ê 5 R ª~ 2003j 5ú¦V 2004j 12úræ þ "æ . þ2j ~Ö ·îË 7öB M. hyo ê Ú r, M. hyoö & W V ìº ·îËj Fê ~& . ''~ ·îËö & ;B ~Ö>Úê Æ , æ, þ2~ ¢_ j J~& . Æº ê.ö 8¢ *(3ú-5ú), ª(6ú-8ú), &j(9ú-11ú), ÎÞ (12ú-2ú) \ª~ V~&b, æf ãVê, Ï Óê, *¢ê, ãçê 4B ê * \ª~& . ¢ _ ;~ ãÖ ÖÒ¾¢öB ¢>'b ÒÏ>Úæº ÒGê \ªö 8¢ Îî, F¶î(<30¢_), F¶ î(30-70¢_), GWî(>70¢_)~ 4&æ~ ê ¾* Ú \ª~&b, ''~ ÒGêö ~º BÚ þ2 7 5B¢ ª~ ºÂ~& . Ûê' ºÂ~ Þ~¢ *~, ''~ ·îËf ÒGêö 8¢ \ ª>Ú r, ·îËî Î ÒGê¢ þ2B <º ©öB¦V ~¾~ ÒGêòj <º ©ræ. ·~² ¾æÒ . -² ÒGêö 8¢ \ªB ³Ë~ ;º îÚ *(farm unit)B ÒÏ >î, farm unitº ê., æ ;f þ ß; Ò Gêö ~º 5B~ .Ó þ2j ~² ~&. . C 148B~ ³Ë Fê>îb, 235B~ farm unit \ª>î, C 1,175B~ .Ó þ2 ¦Òö ÒÏ>î . Ûê ¦Ò (1) ·îË *öB~ M. hyo F÷N Ò B B ¢^öB ÖÒ¾¢ GWî~ ãÖ 50% ç~ ·WNj &bæ [8], 95% ÖêB ³Ë ~ M. hyo 6" ¦¢ 6~V *Bº 5B~ þ2 jº~² >î . Îî~ ãÖ, GWî~ ãÖf FÒ ©b '~, 95% ÖêB 5B~ þ2 ÷~ ·W ¦¢ 6~º jº >¢ 6 ~& . ª~ ö ''~ farm unit 5B~ þ 2j ~&bæ *~ j ò~& . 8¢B 5 B~ þ2 7öB 1B ç~ *7 ELISA ·W BÚ& ¾æÆ ãÖ farm unit¢ ·W ·îËb *"~&. . -² Îjê Îî GWîö ~º 116B~ farm unit¢ ºÂ~ M. hyo ·WNj êÖ~&{(· W farm units/Îî GWîö ~º *Ú farm units) Ü100}, ÖÒ¾¢ ·îË~ M. hyo F÷Nj ê Ö~& . ·îËöB~ F÷N öò jî¢ ÖÒ¾¢ î`~ *>' M. hyo F÷Nj rjV *~ Ò.

(5) ÖÒ¾¢ ·îËö* Mycoplasma hyopneumoniae F÷N `Ò. Gêö çì, þ2B Îjê *Ú 1,175B~ . ÓöB ·W .Ó~ jNj êÖb ÖÒ¾¢ î ` Ú M. hyo F÷Nj êÖ~& . (2) î`~ ÒGêê M. hyo F÷N Ò .>Úê 235B~ farm unit¢ Îî,

(6) F¶î, F ¶î, G9îö V¢ ª~~& . '' 59, 42, 77, 57B ~ farm unitb ¾~Úrb, '' 295, 210, 385, 285 B~ BÚ þ2>¢

(7) ~& . 5B~ BÚ þ2 7ö 1B ç~ ·9 ¾æÂ farm unitº ·9b $~ & (95% Öê). .Ó Ú M. hyo Ú·9 ¦f G 9ê*~ çê¢ ª+~8 *~ χ ¦;O»j Ï~& . (3) î`~ æê M. hyo F÷N Ò *Ú 235B~ farm unitö* ã8ê, ÏÓê, *¢ê, ãçêö ~º © j ºÂ~& . '' 66, 49, 14, 51B~ farm units& Îjrb, 330, 245, 70, 255B~ BÚ þ2>¢

(8) ~& . .Ó Ú M. hyo Ú·9 ¦f æ æ>*~ çê¢ ª+~8 *~ χ ¦ ; O»j Ï~& . r, ÒGêö V F÷N ~ N¢ J~8 *~, ' æö* Îjê þ2j ÒGêê \ª ê, ç7 ;O»j Û~ æ ê ·9N~ 8j ;~& . ß;æö*~ ç7 ;~ = (sr1×S1+sr2×S2+sr3×S3+sr4×S4) ÝN 2. 2. 57. Ö . ·îË *~ . IZP F÷N î`Ú BÚ *~ . IZP F÷N `Ò Îî G9îö ~º 116B~ farm unit* Ö Ò¾¢ ·îËö* M. hyo F÷Nj êÖ Ö, Ö Ò¾¢ ·îË~ 85.34%(95% CI, 78.90-91.78%)& M. hyoö 6"B ©b ¾æÒ . 1,175B~ BÚ .Ó¦ Ò¢ Û î`Ú BÚ F÷Nf 34.81%(95% CI, 32.0937.53%) ¾æÒ (Table 1). æ~ ÒGêê . IZP F÷N jL`Ò ·îË *~ ãÖ, Îî G9î(>70¢_)~ F÷ N '' 83.05%f 87.72%* ¸² ¾æÒb,

(9) F¶î(<30¢_)~ ãÖ 28.57% &Ë Ô² ¾z. (Table 2). χ ¦; Ö, χ = 51.9405* ÒGêf · 2. 2. Table 1. Seroprevalence of Mycoplasma hyopneumoniae infection in Korean swine farms from May 2003 to December 2004 Prevalence. Total number (A). Positive number (B). B/A(%) (95% CI1)). Among Korean swine farms (No. of farm unit). 116. 99. 85.34 (78.90-91.78). Among pigs (No. of pigs). 1,175. 409. 34.81 (32.09-37.53). 1). 95% confidence interval.. sr1, sr2, sr3, sr4 =. ß; æ ö* Îî,

(10) F¶î, F¶î, G9î ~ ·9 þ jN. S1, S2, S3, S4 = N=. Îî,

(11) F¶î, F¶î, G9î ~ + þ > *Ú þ >. î`~ ê.ê F÷N Ò B~ ö* * ú ª(6-8ú), &j(9ú ÎÞ ú ö ~º © j ºÂ~& . ' ' B~ & Îjrb, 300, 305, B~ BÚ þ2>¢

(12) ~& . .Ó Ú M. hyo Ú·9 ¦f ê. æ>*~ çê¢ ª+~8 *~ χ ¦;O»j Ï~& . r, ÒGêö V F÷N~ N¢ J~8 *~, ' ê.ê Î jê þ2j ÒGêê \ª ê, *ö* 8. ç7 ;O»j Û~ ê.ê ·9N~ 8j ; ~& .. Table 2. Seroprevalence of Mycoplasma hyopneumoniae infection by production stage in Korean swine farms Production unit survey Production stages. M. hyo (4) 235 farm unit , (3-5 ), 11 ), (12-2 ) 60, 61, 70, 30 farm units 350, 150 2. Selected Positive farm units farm units (A) (B). B/A(%) (95% CI1)). Sow. 59. 49. 83.05 (73.48-92.62). Suckling piglet (<30 days old). 42. 12. 28.57 (14.91-42.23). Nursery pig (30-70 days old). 77. 39. 50.65 (39.48-61.82). Growing pig (>70 days old). 57. 50. 87.72 (79.20-96.24). 1). 95% confidence interval. χ2 = 51.9405, p<0.005..

(13) B*²ÁB[Áª«9Á·^Á;/. 58. Table 3. Seroprevalence of Mycoplasma hyopneumoniae by production stage in Korean pigs. Table 4. Serorevalence of Mycoplasma hyopneumoniae by region in Korean pigs Individual survey. Individual survey. Production stages. Selected Positive samples(A) samples(B). Sow. 295. Suckling piglet Nursery pig. 172. 210. 18. 385. Growing pig. 81. 285. 138. B/A(%) (95% CI1)) 58.30 (52.67-63.93) 8.57 (4.78-12.36) 21.04 (16.97-25.11) 48.42 (42.62-54.22). Selected Positive Crude Adjusted rate2) (%) samples samples rate1) (A) (B) (B/A, %) (95% CI3)). Province. Gyeonggi-do. 330. 117. 35.45. 30.46 (25.49-35.43). Chungcheong-do. 245. 95. 38.78. 40.90 (34.74-47.06). 70. 27. 38.57. 25.93 (15.66-36.20). 255. 93. 36.47. 36.13 (30.23-42.03). Chunla-do Kyungsang-do. 1). 95% confidence interval. χ2 = 190.9177, p<0.005.. 1). (Positive samples/Selected samples)×100. Value that production stage is considered with direct standardization. 3) 95% confidence interval. χ2 = 0.7705. 2). îË~ F÷N Òöº F~' ç&&ê& ®r ¦;>î (p<0.005). BÚ *öB ÒGêê F÷Nf ·îË *öB ~ NZ" jÝ~² ¾z (Table 3). BÚ * Òö Bº χ ¦; Ö", χ = 190.9177B ÒGêf BÚ ê ·WN *~ ç&&ê& Ö ¸² ¾æÒ (p<0.005). Îî~ ãÖ GWîö j ·WN £ 10% ;ê ¸ ² ¾æÒb¾ 95% Ö* 'Ï F~' Ö" B ææº p~ .

(14) F¶î~ ãÖ 8.57%B & Ë Ôf F÷Nj ¾æî . æ ê . IZP F÷N jL`Ò ã8ê, ÏÓê, *¢ê, ãçêöB îÚ M. hyo F ÷Nf 30.46%, 40.90%, 25.93%, 36.13%B ¾æÒ. . ÏÓêöB F÷N &Ë ¸² ¾æÒb¾, 95% Ö* 'Ï F~W ®º Ö" ªC>Úææ p~. (Table 4). χ ¦; Ö", χ = 0.7705, p>0.05B ã8 ê, ÏÓê, *¢ê, ãçê~ æ æ>f .Ó¦Ò M. hyo ·WN"º F~' ç&&ê& ìº ©b ¾æÒ . ê.ê . IZP F÷N jLÒ *(3-5ú)ö 24.11%B &Ë Ôf F÷Nj &, ÎÞ(12-2ú)ö 45.63%B &Ë ¸f F÷Nj ¾æî. (Table 5). χ ¦; Ö", χ = 31.4741, p<0.005B ê . æ>f M. hyo ·WN"º ç&&ê& ¸² ¾æÒ. . ÚB, ' ÒGêö 8 ê.ê M. hyo ·WN ~ N¢ Table 6ö ¾æÚî . Îî"

(15) F¶î~ ã Ö .&' >~ N& ®îæò ê.ê ·WN~ æ 2. 2. 2. 2. Table 5. Seroprevalence of Mycoplasma hyopneumoniae by season in Korean pigs Individual survey Season. Spring (Mar-May). 300. 74. 24.67. 24.11 (19.27-28.95). Summer (Jun-Aug). 305. 120. 39.34. 35.72 (30.34-41.10). Fall (Sep-Nov). 350. 108. 30.86. 35.64 (30.62-40.66). Winter (Dec-Feb). 150. 73. 48.67. 45.63 (37.66-53.60). 2. 2. Selected Positive Crude Adjusted samples samples rate2)(%) rate1) (B/A, %) (95% CI3)) (A) (B). 1). (Positive samples/Selected samples)×100. Value that production stage is considered with direct standardization. 3) 95% confidence interval. χ2 = 31.4741, p<0.005. 2). z ·çf jÝ~² ¾æÒ . ¯, *" ÎÞ ª " &jö ·WN ç&'b ¸² ¾æÒ . F¶ î" GWîf '' *ö 8.89%, 32.73%B &Ë Ôf ·WNj ¾æÚî, ÎÞö 40.00%, 70.00%B &Ë ¸f ·WNj ¾æÚî ..

(16) ÖÒ¾¢ ·îËö* Mycoplasma hyopneumoniae F÷N `Ò. 59. Table 6. Seasonal positive rates by production stage in Korean pigs Season Spring(Mar-May) Summer(Jun-Aug). Fall(Sep-Nov). Winter(Dec-Feb). Sow. Total samples Positive samples Positive rate1), %. 90 43 47.78. 110 71 64.55. 55 36 65.45. 30 17 56.67. Suckling piglet (<30 days old). Total samples Positive samples Positive rate, %. 60 3 5.00. 45 4 8.89. 75 10 13.33. 20 1 5.00. Nursery pig (30-70 days old). Total samples Positive samples Positive rate, %. 90 8 8.89. 90 20 22.22. 130 24 18.46. 50 20 40.00. Growing pig (>70 days old). Total samples Positive samples Positive rate, %. 55 18 32.73. 60 25 41.67. 90 38 42.22. 50 35 70.00. 1). (Positive samples /Total samples)×100. 8. Yagihashi [16]~ ö 8, M. hyoö & Ú·* "æ~ ÒG Bú >ö 8¢ Ã&~¾, 2Bú _~ "æöBº &Ë Ôf M. hyo F÷Nj ~& . $ Wallgren [15]f ·îËöB îz2 ¢®îöZ B~z¢ê 4-7"_ö 8 Ã^¢ º "æöBº .Ó¦Ò Ú·* BÒ>æ p º, F& ÎÚ¯Ú~ ² " £ ÚÖ ËK H^¢ ~& . 8¢B F¶îêöBº . Ó¦Ò¢ Û M. hyo ¶6"¦¢ ;{® C&ÚV & ÚJÖæ, .Ó' Ò, Û 3Bú_öB 4B ú_ö æº Ú·* M. hyo~ ¶6"j z " > ® [16]. .Ó¦Ò, ELISAº îz2 ¢®îöZö & ÎîVç ¦Ò»b FÏ~,. Mycoplasma «"~ vN>wj ²z ©b Ò ÏB v kit*ö ·WBÚ¢ 6ê~ºº F~ N ¢ æ pj ® ~ Ö"ö ÒÏB kit~ 'Ë f ìj ©b '>Ú ê . ÖÒ¾¢ *Ú ·îËöB~ M. hyo F÷N" îÚ BÚ*~ M. hyo F÷Nf '' 85.34%, 34.81% ¾ æÒ . º ÖÒ¾¢ ·îËö M. hyo& çÒ ®r j ¾æÞ ' > ® . ß®, î Ú BÚ* ~ F÷N ·îË *~ F÷Nö j Ô² ¾æÒ º, º "æ~ "_ö 8¢ M. hyo Ú·*ö N & VV H^ . f f

(17) F¶î" F¶îö B~ ·WNf GWîö j ç&'b ÔV H^ö [8]

(18) F¶î" F¶îf ·îË~ M. hyo ¶6". Nj ;{® >'~æ á > ® ~& . 8¢B ·îËöB M. hyo ¶6" FZ¢ 6~V *~ Îî" GWîö ~º farm unit¢ ºÂ~& . ' ÒGêê M. hyo Ú·W ·îË~ ª

(19) ¢ r j Ö", Îî" GWîöB ·Wj ·îË~ > & ô~ . º &¦ª~ ³Ë M. hyo 6" ·W GWî" Îîj F~ ®rj & . ß® G WîöB M. hyo Ú·W ³Ë~ jN 87.72%B 7*8b jv ~j H, &Ë ¸² ¾æÒ . Î îf Ú·WjN ¸ ~z¢ê M. hyo~ *2ö ` V¢ ~æ p [5, 12], all-in/all-out ÒG O ê « M. hyo *2& 6²® º Ú " H [3], Îî º GWî M. hyo~ *2ö ®ÚB 7º j ~º ©b 'B . Ò, M. hyo~ ã Ö V*2& &Ë~ º Ú " H [13], * >Ï~º GWîöB M. hyo~ control B& Úææ pj ãÖ, ·îËöB M. hyo~ òW' 6" ç& ê³F > ®rj z . ' ÒGêöB~ M. hyo ·W BÚ~ jNf · îË *~ ÒöBf jÝ ·çb ¾æÒ . Î î GWîöB~ F÷N &Ë ¸~,

(20) F ¶î~ F÷Nf 10% ~ Ô² ¾z . M. hyoö & Î Ú¯Úº & 9"ræ æ³F > ®Ú [10]

(21) F¶ î(<30¢_)öB ·W BÚ~ Â*f Îv ÎÚ¯ Úö ~ ©b " >ê ® . M. hyoö ~ 6"ö B Ú·* Fê>Vræ Û 3-8" ;ê Òæ [9, 10, 11], ÎÚ¯Ú& &¦ª ² >º F¶î öV(8-10"_)ö ¶6" Ã&~V ·~, GW.

(22) B*²ÁB[Áª«9Á·^Á;/. 60. îöB M. hyo F÷N ¸² Ã& ©b '>Ú ê . º fö ~ Úê ÒÖf ¢~~º ©b [8], F¶îV(30-70¢_)öº

(23) F¶îV(<30 ¢_)ö j ·W BÚ~ jN 21.04% ¸² ¾æ Ò . BÚ * Úê æê M, hyo F÷N Òö Bº, ãVê, ÏÓê, *¢ê, ãçê~ ææ>f M. hyo ·W ¦fº W ìº ©b ¾æ¾ Ö Ò¾¢ ßF~ ·î~ã LÛÞ~, Ò· Ê~ ¢ NWj z " > ® . BÚ * Úê ê.ê M. hyo F÷N ÒöBº ê. æ>f M. hyo ·W ¦f F~' çê& ®r C&^, ê. ö 8 *Ú î`öB F÷N~ N& F¶î G Wî~ ê.ê F÷N~ æz ·ç &7 N ®. º ©j & . ÒGêö 8 F÷N ÒöB &¦ª~ ¶6" F¶î öVf GWîöB Úæ ®º ©b ¾æ¾ ê.ê F÷N~ Nº Ö Ò¾¢öB M. hyo ¶6" B~º V¢ &Û' b º/ > ®º ¶ò& F > ® . æ, Î Þ(12-2ú)ö 45.63%B &Ë ¸f F÷Nj &bæ 6" ê Ú·* Úæº 3-8"¢ J~&j H, &Û Ì&jöB .ÎÞö ¶6" ç&'b ¾ B ©b º;B .. Ö V. 2003j 5ú¦V 2004j 12úræ BÞ&L >~ & Öëÿb:Ê þ ~Ö>Úr~ .Ó þ 2 7ö M. hyo Wj ÒÏ~æ pº 148B~ ·îË öB 1,175B~ .Óþ2j ºÂ~& . ' ·îËf æ ê.ö & ;¢

(24) ~&, ÒGêö 8¢ Îî,

(25) F¶î(<30¢_), F¶î(30-70¢_), GWî (>70¢_)b ª~>Úr . -² ê., æ, ÒG ê~ ;¢ <º *¢ farm unit ~ Ûê Òö Ï>Úr . Îî GWîö ~º 116B~ farm unitöB 85.34%(78.90-92.78%, 95% CI)~ ·WNj ¾æÚî, ÒGêö çì, *Ú 1,175B~ .ÓöB 34.80% (32.09-37.53%, 95% CI)~ F÷N(seroprevalence)j ¾æ Úî . ÒGêö 8 M. hyo F÷Nj Ò Ö, Îî GWîöB 83.05%(73.48-92.62%, 95% CI), 87.72% (79.20-96.24%, 95% CI)~ ·îË M. hyoö 6">Ú ®º ©b ¾æÒ . Ò BÚ * Ò Ö, 810"_~ "æöB ¶ 6" Ã&~V · º © j r > ®î .. æê M. hyo F÷Nj Ò Ö, ãVê, Ï Óê, *¢ê, ãçê ~ æ æ>f M. hyo ·WN *ö F~' çê& ìº ©b ¾æÒ (χ = 0.7705). ê.ê M. hyo F÷N ÒöBº, ÎÞ(12-2ú)ö F ÷N F~'b ¸² ¾æÒ (χ = 31.4741, p<0.005). 6" ê Ú·* ÚæVræ~ V¢ J " H, Ì&jöB .ÎÞö ¶6" ç&'b ¸ ² B ©b '>Ú ê . 2. 2. ^^ò 1. Blanchard B, Vena MM, Cavalier A, Le Lannic J, Gouranton J, Kobisch M. Electron microscopic observation of the respiratory tract of SPF piglets inoculated with Mycoplasma hyopneumoniae. Vet Microbiol 1992, 30, 329-341. 2. Cho KH, Choi JS, Kim BH. Survey on mycoplasmal pneumonia of swine in Yongnam area and antimicrobial susceptibility of Mycoplasma hyopneumoniae isolated from slaughter pigs. Korean J Vet Res 1998, 39, 96103. 3. Clark LK, Scheidt AB, Armstrong CH, Knox K, Mayrose VB. The effects of all-in/all-out management on pigs from a herd with enzootic pneumonia. Vet Med 1991, 9, 946-951. 4. Dungworth DL. The respiratory system. In: Jubb KFC, Kennedy PC, Palmer N(ed.). Pathology of Domestic Animals, 4th ed., pp. 661-663, Academic Press, New York, 1993. 5. Gardner IA, Hird DW. Host determinants of pneumonia in slaughter weight swine. Am J Vet Res 1990, 51, 1306-1311. 6. Goodwin RFW, Pomeroy AP, Whittlestone P. Production of enzootic pneumonia in pigs with a mycoplasma. Vet Rec 1965, 77, 1247-1249. 7. Kazama S, Yagihashi T, Seto K. Preparation of Mycoplasma hyopneumoniae antigen for the enzymelinked immunosorbent assay. Can J Vet Res 1989, 53, 176-181. 8. Kim HK, Kim EM, Moon HJ, Kim TY, Lim JS, Lee YH, Park BK. Analysis of Mycoplasma hyopneumoniae infection by seroepidemiological investigation in Korean swine herds. Korean J Vet Res 2004, 44, 587-591. 9. Kobisch M, Blanchard B, Le Potier MF. Mycoplasma hyopneumoniae infection in pigs: duration of the disease and resistance to infection. Vet Res 1993, 24,.

(26) ÖÒ¾¢ ·îËö* Mycoplasma hyopneumoniae F÷N `Ò 67-77. 10. Morris CR, Gardner IA, Hietala SK, Carpenter TE, Anderson RJ, Parker KM. Seroepidemiologic study of natural transmission of Mycoplasma hyopneumoniae in a swine herd. Prev Vet Med 1995, 21, 323-337. 11. Nicolet J, Zimmerman W, Chastonay M. Epidemiology and serodiagnosis of Mycoplasma hyopneumoniae. Zentralblatt Bakterol 1990, 20(suppl.), 249-253. 12. Sheldrake RF, Gardner IA, Saunders MM, Romalis LF. Serum antibody response to Mycoplasma hyopneumoniae measured by enzyme-linked immunosorbent assay after experimental and natural infection of pigs. Aust Vet J 1990, 67, 39-42. 13. Stark KD, Nicolet J, Frey J. Detection of Mycoplasma hyopneumoniae by air sampling with a nested PCR. 61. assay. Appl Environ Microbiol 1998, 64, 543-548. 14. Vicca J, Maes D, Thermote L, Peeters J, Haesebrouck F, de Kruif A. Patterns of Mycoplasma hyopneumoniae infections in Belgian farrow-to-finish pig herds with diverging disease-course. J Vet Med B 2002, 49, 349353. 15. Wallgren P, Bolske G, Gustafsson S, Mattsson S, Fossum C. Humoral immune responses to Mycoplasma hyopneumoniae in sows and offspring following an outbreak of mycoplasmosis. Vet Microbiol 1998, 60, 193-205. 16. Yagihashi T, Kazama S, Tajima M. Seroepidemiology of Mycoplasmal pneumoniae of swine in Japan as surveyed by an enzyme-linked immunosorbent assay. Vet Microbiol 1993, 34, 155-166..

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