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Effects of propofol-induced autophagy against oxidative stress in human osteoblasts

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Fig. 1. The in vitro experimental protocols. Control = normal incubation, H 2 O 2  = normal incubation after H 2 O 2  treatment, PPC/H 2 O 2  = propofol  preconditioning (PPC) before H 2 O 2  treatment, 3-MA/PPC/H 2 O 2  = 3-methyladenine treatment followe
Fig. 2. Effect of propofol and 3-MA on cell viability. (A) The viability of propofol-treated hFOB cells
Fig. 5. Flow cytometry analysis of apoptosis and necrosis with Annexin- Annexin-V-FITC and propidium iodide staining
Fig. 9. Western blot analysis. Expression of collagen type I, bone  morphogenic protein (BMP)-2, osterix, and transforming growth  factor-beta (TGF-β)1 in the experimental groups indicated

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