WCIM 2014
34 32nd World Congress of Internal Medicine (October 24-28, 2014)
OS-IFD-10 Infectious Disease
Prototype Design of Lymphocyte TCD4+ Resistant Against HIV Infection Generated from Peripheral Blood Haematopoietic Stem Cell (PBMCs)
Purwati PURWATI1 Roche Indonesia, Indonesia1
Background: AIDS epidemic has spread to all parts of Indonesia and currently more than 150 countries reported the existence of HIV/AIDS from around the world. Addi- tionally, HIV/AIDS treatment using ARV drugs also fi nd obstacles that must be faced in terms of host, environment and the agent. The objective of this study was to generate lymphocytes TCD4+ that are resistant to HIV infection generate from PBMCs through by deletion of 32 bp CCR5 encoding gene.
Methods: In principle, this study was done in three steps. First, isolation, culture and purifi cation of lymphocyte TCD4+ from PBMC (Mather, 2008; Rantam, et.al., 2009).
Second, lymphocyte TCD4+ characterization by PCR with primer F 5’CAAGTCGAG- CGCCCCGCAAGGGG-3, R 5’GTCCGAGTGTGGCTGATCATCC-3 (Thomsen, et.al., 2002;
Yuwono, 2006; (Hall and Ziedonis 2007; Purwati, et.al., 2009). Third, designing of lym- phocyte TCD4+ prototype which was resistant to HIV infection by deletion of 32 bp CCR5 full gene.
Results: Twenty-four hours after culture, there were abundant cell growths. TCD4+
lymphocytes from isolated and cultured 10 ml PBMC were found to be 2 x 107. Phe- notype characterization of TCD4+ lymphocyte provided positive results, while the genotype showed similarities to that in corresponding gene bank of CCR5 variant A and variant B. Prototype of HIV resistant TCD4+ lymphocytes was made by nucleotide deletions in conserved areas, at position 554-576 bp, using restriction enzymes EcoRI checked using PCR and sequencing. In conclusion, prototype design of HIV resitent TCD4+ lymphocytes is obtained through the deletion of 32 bp CCR5 encoding full gene at GTCAGTATCAATTCTGGAA GAATTT CCAGACA using EcoRI enzyme.
OS-IFD-11 Infectious Disease
Psychiatric Morbidity Among HIV-Infected Children and Adolescents in a Resource-Poor Kenyan Urban Commu- nity
Njeri Rahab MBUGUA1, Asunta Wagura WAGURA2, Elizabeth Ann BUKUSI1, Judy W KAMAU4, Wangari KURIA3, Muthoni MATHAI4
Kenya Medical Research Institute, Kenya1, Kenya Network of Women Living with HIV/AIDS, Kenya2, Kenyatta National Hospital, Kenya3, Nairobi University, Kenya4
Background: The course of HIV/AIDS in children has been transformed from an acute to a chronic one with the advent of Anti-Retroviral Therapy.
Objectives: The aim of this study was to determine the prevalence and pattern of psychiatric morbidity in HIV-infected children and adolescents between 6 and 18 years of age and the relationship between their socio-demographic factors, immune sup- pression and psychiatric morbidity.
Methods: The study was conducted at a ( Kenya Network of Women Living with HIV/
AIDS) pediatrics HIV clinic in Nairobi, between February and April 2010. One hundred and sixty-two HIV-infected children and adolescents aged between 6 and 18 years and their guardians were interviewed.
Results: Seventy-nine (48.8%) of the study participants were found to have psy- chiatric morbidity. The most prevalent Diagnostic Statistical Manual, 4th Edition TR psychiatric disorders were: Major depression (17.8%), Social phobia (12.8%), Opposi- tional Defi ant Disorder (12.1%) and Attention Defi cit Hyperactivity Disorder (12.1%).
Twenty-fi ve per cent of the study participants had more than one psychiatric disorder.
Conclusions: The prevalence of psychiatric morbidity in HIV-infected children is higher than that found in children in the general population. There is therefore a need to in- tegrate psychiatric services into the routine care of HIV-infected children.
OS-NEP-01 Nephrology
Dipping Status in Individuals Monitored by Ambulatory Blood Pressure Measurements
Hakan SARLAK1, Fatih BULUCU1, Erol ARSLAN1, Seref DEMIRBAS1, Kenan SAGLAM1 Internal Medicine, Gulhane Military Medical Academy, Turkey1
Background: Blood pressure normally decreases during the night-defi ned as ‘dipping’.
The incidence of cardiovascular events is higher in patients with a lesser drop in noc- turnal blood pressure than in those with greater drop (ESC 2013). We aimed to deter- mine the status of dipping and to compare the dipping status in different subgroups of the measurements from individuals ambulatory monitored for blood pressure.
Methods: A total of 692 24-h ambulatory blood pressure measurements, consisting of 393 hypertensives and 299 normotensives (male:334 measurements, female:358 measurements) were included in to the study. Dipping status (night-day systolic blood pressure ratio) was investigated in all measurements.
Results: Mean systolic blood pressure and diastolic blood pressure were 130.2 ± 14.1 mmHg and 79.2 ± 10.1 mmHg on average, respectively. In the comparison of meas- urements of 30-64 years old and = 65 years old mean systolic and diastolic blood pressure (129.8 ± 13.82 and 132.17 ± 15.60; 80.44 ± 10.06 and 73.06 ± 8.17, respec- tively), mean diastolic blood pressure was signifi cantly low (p <0.001). Decline of night systolic blood pressure was signifi cantly less in both hypertensive and normotensive women than those of hypertensive and normotensive men (7.74±11.51 and 7.88±8.74 vs 12.48±10.46 and 10.13±9.15, p<0.001; p=0.008, respectively). Also, dipping ratio was signifi cantly lower in the subgroup of hypertensive women aged = 65 years than those of aged 30-64 years (Table 1).
Conclusions: According to these data, dipping rate was found significantly lower in hypertensive women = 65 years old than those of younger hypertensive women.
Considering the low rate of dipping, the presence of an evening dose seems to be important in elderly hypertensive women when arranging the treatment for their hy- pertension. In monotherapy, divided doses will be rational.
