52
-Abstract
Objectives. In this study, we investigate that methanol extract of Euphorbiae lathyridis Semen contributes to growth inhibitory effect on the HT-29 human colon cancer cells. Methods. Euphorbiae lathyridis Semen (ELS) was extracted with 80% methanol. HT-29 cells were treated with different concentrations of ELS extract for 24-72 hrs. Growth inhibitory effect was determined by MTT assay. Cell apoptosis was determined by surveying caspases cascades activation using Western blot. Cell cycle arrest was analyzed by flow cytometry with PI staining.
Results. Exposure to ELS extract showed in inhibitory effects on HT-29 cell growth as a dose-dependent manner. Cell growth inhibition by ELS extract was related with induction of cell apoptosis with DNA fragmentation through the activation of caspases-3, caspase-9 and PARP cleavage.
Conclusion. ELS extract significantly inhibited cell growth and induced cell apoptosis in HT-29 human colon cancer cells, therefore, These results suggest that ELS extract can be used as chemoprevention agent of colon cancers.
Key words: Euphorbiae lathyridis Semen; HT-29 human colon cancer cell; apoptosis
속수자 추출물의 HT-29 대장암세포 증식에 대한 억제효과
Inhibitory effects of Euphorbiae lathyridis Semen extract on cell growth in HT-29 human colon cancer cells
Hyo Won Jung, Yong-Ki Park
Oriental Medicine Drug R&D Center, College of Oriental Medicine, Dongguk University, Gyeongju 780-714, South Korea.
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24 48 72 0 20 40 60 80 100 50mγ/ml 100μg/ml 200μg/ml 300μg/ml 400μg/ml Culture time (h) C e ll v ia b ility ( % c o ntr o l) ± ±
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-PARP 116KDa
89KDa
45KDa
con 0.1 0.2 0.4
Euphorbiae lathyridis Semen (mg/ml) β-actin PARP 116KDa 89KDa 45KDa con 0.1 0.2 0.4
Euphorbiae lathyridis Semen (mg/ml) β-actin con 0.1 0.2 0.4 Procaspase 9 Procaspase 3 β-actin 47KDa 32KDa 45KDa Euphorbiae lathyridis Semen
(mg/ml) con 0.1 0.2 0.4 Procaspase 9 Procaspase 3 β-actin 47KDa 32KDa 45KDa Euphorbiae lathyridis Semen
(mg/ml)
con 100 200 400
Euphorbiae lathyridis Semen (mg/ml)
con 100 200 400
Euphorbiae lathyridis Semen (mg/ml)