• 검색 결과가 없습니다.

Inhibitory effects of Euphorbiae lathyridis Semen extract on cell growth in HT-29 human colon cancer cells

N/A
N/A
Protected

Academic year: 2021

Share "Inhibitory effects of Euphorbiae lathyridis Semen extract on cell growth in HT-29 human colon cancer cells"

Copied!
9
0
0

로드 중.... (전체 텍스트 보기)

전체 글

(1)

52

-Abstract

Objectives. In this study, we investigate that methanol extract of Euphorbiae lathyridis Semen contributes to growth inhibitory effect on the HT-29 human colon cancer cells. Methods. Euphorbiae lathyridis Semen (ELS) was extracted with 80% methanol. HT-29 cells were treated with different concentrations of ELS extract for 24-72 hrs. Growth inhibitory effect was determined by MTT assay. Cell apoptosis was determined by surveying caspases cascades activation using Western blot. Cell cycle arrest was analyzed by flow cytometry with PI staining.

Results. Exposure to ELS extract showed in inhibitory effects on HT-29 cell growth as a dose-dependent manner. Cell growth inhibition by ELS extract was related with induction of cell apoptosis with DNA fragmentation through the activation of caspases-3, caspase-9 and PARP cleavage.

Conclusion. ELS extract significantly inhibited cell growth and induced cell apoptosis in HT-29 human colon cancer cells, therefore, These results suggest that ELS extract can be used as chemoprevention agent of colon cancers.

Key words: Euphorbiae lathyridis Semen; HT-29 human colon cancer cell; apoptosis

속수자 추출물의 HT-29 대장암세포 증식에 대한 억제효과

Inhibitory effects of Euphorbiae lathyridis Semen extract on cell growth in HT-29 human colon cancer cells

Hyo Won Jung, Yong-Ki Park

Oriental Medicine Drug R&D Center, College of Oriental Medicine, Dongguk University, Gyeongju 780-714, South Korea.

(2)
(3)

51 -℃ × ℃ × × × ℃ ×

(4)

24 48 72 0 20 40 60 80 100 50mγ/ml 100μg/ml 200μg/ml 300μg/ml 400μg/ml Culture time (h) C e ll v ia b ility ( % c o ntr o l) ± ±

(5)

53

-PARP 116KDa

89KDa

45KDa

con 0.1 0.2 0.4

Euphorbiae lathyridis Semen (mg/ml) β-actin PARP 116KDa 89KDa 45KDa con 0.1 0.2 0.4

Euphorbiae lathyridis Semen (mg/ml) β-actin con 0.1 0.2 0.4 Procaspase 9 Procaspase 3 β-actin 47KDa 32KDa 45KDa Euphorbiae lathyridis Semen

(mg/ml) con 0.1 0.2 0.4 Procaspase 9 Procaspase 3 β-actin 47KDa 32KDa 45KDa Euphorbiae lathyridis Semen

(mg/ml)

con 100 200 400

Euphorbiae lathyridis Semen (mg/ml)

con 100 200 400

Euphorbiae lathyridis Semen (mg/ml)

(6)
(7)
(8)
(9)

참조

관련 문서

In this study, we investigated the effects of low-power CO 2 laser on proliferation on human gingival fibroblast cells so that determine laser

Results: The human breast cancer cell subline MCF-7/MX5 cells selected in the presence of 5 µg/ml mitoxantrone (MX) were more resistant to MX (15.7... Western blot and

In this study, two experiments were conducted to understand the effects of additional charge on the detailed growth mechanism of Alq 3 and to determine the effect of

Fig 4 : Inhibitory effects of classified methanol extracts of Acalypha australis the COX-2 protein expression of the human oral cavity carcinoma KB cells....

4 > Effects of Taro on COX-2 expression and iNOS expression(hot water) in human thyroid cancer cells. The cells were pretreated for 48hours with either

In the present study we investigated the proliferation effect of human oral cancer KB cell treated with pulsatilla koreana extract.. We analyzed the effects of this

In the present study, consistent to that report, up-regulation of VEGF mRNA was observed in the colon cancer cell with the acquired resistance to 5-FU and this

The aim of this study was to examine the effects and mechanisms of the new metformin derivative HL156A in human multidrug-resistant cancer cell lines (FADU/PTX,