Korean Journal of Food Science and Technology

KOREAN J. FOOD SCI. TECHNOL. Vol

22.

No.7, pp 824-827 (/990)

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*§t~:i!J-Effect of Fed of Phenolic Acids in Plant on Serum Cholesterol

Concentration in Rats

Young-Su Cho and Chung-Kie Kim

Department of Agriculture Chemistry, College of Agriculture, Dong-A University, Pusan Abstract

Phenolic acids are widely distributed in all plant meteriai. Most of these acids were combined with plant or grain cell wall. On the other hand, it had been reported that p-coumaric acid exhibited hypocholesterolemic activity in rats. We have undertaken a study of these compounds with regard to their effect on the rat. In this study, the effects of phenolic acid on the serum cholesterol level in rats fed with cholesterol free and cholesterol enriched diets were examined. The commercially available phenolic acid were purchased in the experiment. These compounds were incoporated in the diet at a level of 0.2%. These diets were fed for 21 days to male wistar strain rats with a body weight of 80 to 9Og. It was found no significant change in serum cholesterol level in the phenolic acid fed rats in both cholesterol-free and cholesterol-enriched diet.

Key words: phenolic acid, serum cholesterol level

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824

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Table 1. Composition of experimental diets (%)

Dietary group Control

CF CEa)

p-Coumaric acid

CFbl CE

Ferulic acid Caffeic acid

CF CE CF CE Casein p-Coumaric acid Ferulic acid Caffeic acid Corn oil Mineral mixture') Vitamin mixture d) Wheat starch Cellulose powder Cholesterol Sodium cholate Lard Sucrose 20.0 1.0 4.0 1.0 2.0 2.0 70.0 20.0 1.0 4.0 1.0 2.0 2.0 1.0 0.4 5.0 63.6 20.0 0.2 1.0 4.0 1.0 2.0 2.0 69.8 20.0 0.2 1.0 4.0 1.0 2.0 2.0 1.0 0.4 5.0 63.4 20.0 0.2 1.0 4.0 1.0 2.0 2.0 69.8 20.0 0.2 1.0 4.0 1.0 2.0 2.0 1.0 0.4 5.0 63.4 20.0 0.2 1.0 4.0 1.0 2.0 2.0 69.8 20.0 0.2 1.0 4.0 1.0 2.0 2.0 1.0 0.4 5.0 63.4

a) CF: Cholesterol-free diet, b) CE: Cholesterol-enriched diet c) Phillip-Hart's mixture

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0.2 mg. Ca pantothenate 5 mg, p-Amino benzoic acid 5 mg, Nicotinic acid 6 mg, Inositol 6 mg, Choline chloride 200 mg

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Table 2. Food intake, body weight gain, liver weight, and serum lipids of rats given diets containing some phenolic acids .)

Dietary group

Control p-Coumaric acid Ferulic acid Caffeic acid

(02%)

Food intake(g/21 days) Body weight gain(g/21 days) Liver weightCg/100g B.W.) b) Serum lipids(mg/100 ml) Total cholesterol HDL-cholesterol LDL-cholesterol 247.1± 1.7 77.6± 1.7 4.8± 0.3 119.7± 18.8 36.1± 5.4 83.6± 14.3 a) (Mean values with their S.D. for five rats/group)

b) B.W.: Body weitht (0.2%) 249.9± 1.3 76.5± 3.1 4.6± 0.1 119.4± 12.5 28.2± 6.3 91.2± 16.2 (0.2%) 247.4± 1.8 72.8± 2.0') 4.8± 0.2 134.5± 15.2 34.0± 8.4 100.5± 6.7 248.8± 0.9 77.5± 2.1 4.5± 0.3 115.6± 4.7 24.6± 2.4c) 91.5± 5.6

c) Significantly different from the value of control group(p<0.05)

Table 3. Food intake, body weight gain, liver weight, and serum lipids of rats given hypercholesterolemia inducing diets containing some phenolic acids')

Dietary group

Control p-Coumaric acid Ferulic acid Caffeic acid (0.2%) Food intake(g/21 days) 242.6± 2.6

Body weight gain(g/21 days) 86.7± 2.6 Liver weight(g/l00g B.W.) b) 6.5± 0.3 Serum lipids(mg/lOO ml) Total cholesterol 658.6± 74.4 HD L-cholesterol 34.0± 7.4 LDL-cholesterol 652.6± 49.0 Triglyceride 116.0± 31.1

a) (Mean values with their S.D. for five rats/group) b) B.W.: Body weight (0.2%) 242.5± 2.6 83.3± 3.4 6.6± 0.4 665.1± 65.l 31.6± 6.2 633.5± 30.0 113.6± 13.8 (0.2%) 242.7± 2.2 81.2± 1.9 ,) 6.8± 0.4 798.6± 95.6 39.6± 8.2 758.9± 90.5 119.8± 15.6 243.3± 2.2 85.8± 2.8 6.7± 0.5 617.9± 90.3 30.0± 4.0 587.8±98.5 117.7± 17.0

c) Significantly different from the value of control group(p<0.05)

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.'2.'3j,t:.j-.

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J

Choromatography, 1 80. 139 (1979)

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827

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(1978)

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Sci. Food Agric., 35, 657(1984)

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Agric. Food Chem., 37, 345(1989)

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J

Nutrition. 11 3, 546(1983)

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