Feeding Frequency Influences the Growth, Food Consumption, Body Composition and Hematological Response of the Korean Rockfish Sebastes schlegelii

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600 Copyright © 2016 The Korean Society of Fisheries and Aquatic Science pISSN:0374-8111, eISSN:2287-8815

서 론

양식어류의성장은생태계내다양한물리

·

화학

·

생물학적요 인들의영향을받지만

,

^어류가^섭취하는^사료의^양과^섭취^사

료의체내이용효율이가장직접적인영향을미친다

(Buurma

and Diana, 1994).

^사료^비용은^총^생산^경비^중

50-60%

^이상을 차지하기때문에가장경제적인사료공급이이루어져야만

,

^경 영상이득을취할수있다

(Sveier and Lied, 1998; Okumus and Bascinar, 2001).

^특히

,

^해상^가두리^양식의^경우^어류가^소비 하지않고허실되는사료가수계의부영양화를비롯한수질악 화를초래할수있어

(Lupatsch et al., 2003),

^연안^지역^환경오 염방지를위해서도사료공급관리는매우중요하다

.

^즉

,

^어류 의성장률과생존율을높이고

,

개체간크기차이를줄이며허실

되는사료양과노동경비를최소화할뿐만아니라최적의사료

효율을유지할수있는사료공급방법이요구된다

(Kubitz and

Lovshin, 1999).

사료공급횟수는양식어류의먹이섭취량

,

^성장^및^노폐물^배 출량을조절하는데결정적인역할을한다

(Wang, 2007).

^적절 하지못한사료공급횟수로인한사료의과잉또는불충분한공 급은어류의성장률과사료이용효율의감소및생산경비의증 가를초래할수있으며주변환경을오염시키는주원인으로작 용한다

(Lee et al., 2000).

^또한^최적^사료^공급^횟수는^사육^어류 의일간사료섭취량과공급시기에대한정보를제공함으로써 체계적인먹이공급방안을수립할수있다

(Wang et al., 1998).

조피볼락

(Sebastes schlegelii)

^은^연안^정착성^어종이자^넙치 다음으로높은양식생산량을보이는우리나라대표적해산양

조피볼락(Sebastes schlegelii)의 성장, 사료 섭취, 체성분 및 혈액성상에 미치는 사료 공급 횟수의 영향

오승용

^1,2

·박진우

¹

*

1한국해양과학기술원 생태기반연구센터, ²과학기술연합대학원대학교 해양생물학과

Feeding Frequency Influences the Growth, Food Consumption, Body Composition and Hematological Response of the Korean Rockfish

Sebastes schlegelii

Sung-Yong Oh

^1,2

and Jin Woo Park

¹

*

1

Marine Ecosystem and Biological Research Center, Korea Institute of Ocean Science and Technology, Ansan 15627, Korea

2

Department of Marine Biology, Korea University of Science and Technology, Daejeon 34113, Korea

The effects of feeding frequency on the growth, food consumption, body composition, and hematological response of the Korean rockfish Sebastes schlegelii were investigated for 77 days at ambient water temperatures (17.2-24.5℃) in a sea cage in Tongyeong, Korea. Three replicate groups of fish were hand-fed to satiation with a commercial diet in one of four different feeding frequency trials (one meal every 2 days, and one, two and three meals per day). At the end of the experiment, the mean weight gain, specific growth rate, and daily feed intake of fish fed one and two meals per day were significantly higher than those of fish fed one meal every 2 days or three meals per day. The feed efficiency of the fish fed three meals per day was significantly lower than that of the fish in the other groups. The glucose concentration of fish fed one meal every 2 days was significantly higher than that of the other groups. We conclude that the optimum feeding frequency for improving the growth of Korean rockfish weighing 100-200 g reared in sea cages is one meal per day under our experimental conditions.

Key words: Korean rockfish, Feeding frequency, Feed intake, Growth, Hematology

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Licens (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

http://dx.doi.org/10.5657/KFAS.2016.0600 Korean J Fish Aquat Sci 49(5) 600-606, October 2016

Received 13 September 2016; Revised 23 October 2016; Accepted 24 October 2016

*Corresponding author: Tel: +82. 31. 400. 6230 Fax: +82. 31. 406. 2882

E-mail address: [email protected]

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식어종으로서

,

^대부분^종묘^생산^이후^연안^지역의^해상^가두리 에서사육이이루어지고있다

.

조피볼락의최적사료공급횟수 에대한연구로는

Lee et al. (2000), Seo and Lee (2008)

^그리고

Mizanur and Bai (2014)

등이수행한바가있지만

,

조피볼락의 사육이주로이루어지고있는해상가두리에서수행한연구보 고는없다

.

^따라서^본^{연구에서는}^조피볼락^양식이^주로^이루어 지고있는통영지역해상가두리내에서사료공급횟수가조피 볼락의성장

,

^사료^섭취^및^{혈액성상에}^미치는^영향을^파악함으 로써연안지역조피볼락해상가두리사육을위한최적사료공 급횟수에관한기초자료를제시하고자한다

.

재료 및 방법

실험어

실험어는 통영 지역 해상 가두리에서 사육 중인 조피볼락 을 사용하였다

.

^실험에 ^이용하기 ^전 ^실험어는 ^대형 ^가두리

(6×6×6 m)

내에서

2

개월이상순치하였고

,

순치중시판배 합사료

(Aller Aqua Co., Christiansfeld, Denmark: 8.4% moisture, 49.0% crude protein, 10.7% crude lipid, 7.0% ash, and 21.3 kJ/g energy)

^를^하루에^두^번

(09:00

^와

16:00)

^에^걸쳐^만복 공급하였다

.

실험 방법

대형가두리내에서순치후총

1,200

^마리의^조피볼락^미성어

[117.7±1.4 g(

평균

±

표준오차

)]

를무작위로선정하여실험가 두리

(2.0×3.0×2.0 m) 12

^개에

100

^마리씩^분산^{수용하였다}

.

^실 험구는

2

^일

1

^회

(0.5

^회

/

^일

), 1

^일

1

^회

(1

^회

/

^일

), 1

^일

2

^회

(2

^회

/

^일

)

^그 리고

1

일

3

회

(3

회

/

일

)

사료공급구로나누었으며

,

사료공급시 간은

0.5

^회

/

^일와

1

^회

/

^일^공급구는

09:00

^시에

, 2

^회

/

^일^공급구는

09:00

와

16:00

시에그리고

3

회

/

일공급구는

09:00, 12:30

그리

고

16:00

^로^{설정하였다}

.

^사료는^순치기간^중^공급한^사료와^동

일한사료를사용하였으며

,

^각각^설정된^시간에^만복^공급하였 다

.

실험은총

77

일간이루어졌으며

,

실험기간동안사료허실 이되지않도록세심한주의를기울여공급하였다

.

^실험^기간^동 안수온은

17.2-24.5℃

범위이었으며

,

염분과용존산소농도는 각각

31.5-33.5 psu

^와

6.5-10.1 mg/L

^{범위이었다}

.

성장률 및 사료 섭취 패턴

모든실험어는종료시에개체별로무게를측정하였다

.

각실

험어의무게는장

(gut)

^내^남아있는^{대사산물을}^비우기^위해

24

시간동안 절식한후

2-phenoxyehthanol (Sigma, St. Louis, MO, USA)

^용액

(150 mg/L)

^으로^마취하여^{측정하였다}

.

^사료^공 급횟수별조피볼락의성장률측정을위해증체율

(weight gain, WG),

일간성장률

(specific growth rate, SGR),

일간사료섭취율

(daily feed intake, DFI)

^그리고^사료효율

(feed efficiency, FE)

을아래의식으로구하였다

.

증체율

(%)=(W

_f

– W

_i

)×100/W

_i

일간성장률

(%/day)=100×(lnW

_f

– lnW

_i

)/t

일간사료섭취율

(% body weight/day)=100×C/[(W

_f

+ W

_i

)/2]/t

사료효율

=100×(W

_f

– W

_i

)/ C

여기서

, W

_f와

W

_i는최종및최초어류무게

, n

은개체수

, t

는실 험기간

(

^일

)

^그리고

C

는총누적사료량을나타낸다

.

사료공급횟수에따른개체간크기차이

(coefficient of varia-

tion, CV)

^를^조사하기^위해^아래의^식을^이용하여^{측정하였다}

.

CV (%) = 100×(standard deviation of the final weight/mean final weight of the fish in each cage)

사료공급횟수에따른일간사료섭취패턴을조사하기위 해실험

76

일째에각실험구의사료공급시간별먹이소비량 을조사하였다

.

체성분

사료공급횟수에따른조피볼락의체성분변화를조사하기 위해실험시작시무작위로

20

^마리와^실험^종료^시^각^가두리별

무작위로

10

마리씩샘플하여분석하기전

-40℃

냉동고에보관

한뒤

standard method (AOAC, 1990)

^에^따라^{분석하였다}

.

^조 단백질함량은

Kjeldahl method

에따라

Auto Kjeldahl System (Foss Tecator, Hoganas, Sweden)

^을^사용하여^{분석하였고}

,

^수 분은

105℃ dry oven

^에서

24

^시간^건조시킨^다음^{측정하였다}

.

조지방은

ether-extraction method

에따라

,

조회분은

550℃

에 서

4

^시간^연소시킨^후^각각^{측정하였다}

.

혈액 성상

사료공급횟수에따른조피볼락혈액성상변화는실험종료 시각가두리별무작위로

10

^마리씩^샘플하여^혈액^내

glutamic

oxaloacetic transaminase (GOT), glutamic pyruvic transami-

nase (GPT), glucose (GLU), total cholesterol (TCHO), high

density lipoprotein cholesterol (HDLC)

그리고

hematocrit

를 조사하였다

.

^혈액은^실험어를

2-phenoxyethanol (150 mg/L)

로

1

^분간^마취시킨^후

,

^{헤파린으로}^처리된^주사기를^이용하여 미부동맥으로부터채취하였다

.

채취된혈액은

4℃

에서

5

분간 방치한후

Hb

^를^{측정하였고}

, 12,000 rpm

^에서

5

^분간^원심분리 하여혈장을추출하였다

.

그런다음

FUJI DRY- CHEM 4000i

(Fujifilm CO., Japan)

^를^사용하여^추출된^혈장^내

GOT, GPT,

GLU, TCHO

^그리고

HDLC

^를 ^{분석하였으며}

, hematocrit

^는

capillary tube

에혈액을담은뒤

hematocrit centrifuge

로

5,000

rpm

^에서

5

^분간^{원심분리하여}^전체^혈액^중^{혈구비율을}^계산하 여분석하였다

.

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통계 처리

모든통계분석은

SPSS 11.5 (SPSS Michigan Avenue, Chi- cago, IL, USA)

^통계^{프로그램을}^사용하여^분산분석

(ANOVA)

을실시하였으며

, Duncan's multiple range test

^로^평균간^유의 성을

95%

신뢰수준에서검정하였다

.

일간

2

회의먹이섭취패 턴의경우

t-test

^로^평균간^유의성을

95%

^{신뢰수준에서}^검정하 였다

.

결 과

성장률과 사료 섭취 패턴

사료공급횟수에따른조피볼락의어체중변화

,

^증체율

,

^사료 효율

,

^{일간성장률}

,

^{일간사료섭취율}^및^생존율을

Table 1

^에^나 타내었다

.

^실험구

0.5

^회

/

^일과

3

^회

/

^일의^증체율

,

^{일간성장률}^그 리고일간사료섭취율은

1

회

/

일와

2

회

/

일실험구에비해유의하 게낮은

(P<0.05)

반면

,

^일일

1

^회와

2

^회^사이에는^차이가^없었다

(P>0.05).

^{사료효율의}^경우

3

^회

/

^일^실험구가

0.5

^회

/

^일

, 1

^회

/

^일 그리고

2

^회

/

^일^보다^유의하게^낮았지만

(P<0.05),

일일

0.5

^회

, 1

회그리고

2

^회^실험구^사이에는^차이가^없었다

(P>0.05).

^생존 율은사료공급횟수의영향이없었다

(P>0.05).

사료공급횟수 에따른조피볼락의개체간크기차이

(CV)

^는

Fig. 1

^에^나타내 었다

.

^개체간^크기^차이는

2

^회

/

^일까지는^감소하고

3

^회

/

^일^사료 공급구에서다시증가하는경향을보였지만

,

^사료^공급^횟수에 따른차이는없었다

(P>0.05).

사료 공급횟수에따른조피볼락의 일간사료섭취 패턴을

Fig. 2

^에^{나타내었다}

.

^사료^공급^횟수가^{증가할수록}

1

^회^섭취량 은감소하는경향을보였다

.

^일간

2

^회의^실험구의^경우^공급^시 간에따른차이가없었지만

(P>0.05),

일간

3

^회^사료^공급구의 평균사료섭취율은

12:30

^시가

09:00

^시와

16:00

^시^보다^유의하 게낮게나타났다

(P<0.05).

체성분

사료공급횟수에따른조피볼락의체성분변화를

Table 2

^에 나타내었다

.

^수분^함량은

0.5

^회

/

^일와

3

^회

/

^일^실험구가

1

^회

/

^일과

2

^회

/

^일^{실험구보다}^높았다

(P<0.05).

^조단백질^함량은

1

^회

/

^일와

2

회

/

일실험구가

0.5

회

/

일와

3

회

/

일실험구보다유의하게높은

값을 보였다

(P<0.05).

조지방은

0.5

^회

/

^일 ^실험구가

1

^회

/

^일

, 2

회

/

^일^그리고

3

^회

/

^일^보다^유의하게^낮았지만

(P<0.05),

^일간

1, 2

그리고

3

회실험구사이에는유의한차이가없었다

(P>0.05).

조회분함량은

1

^회

/

^일과

2

^회

/

^일^실험구가

0.5

^회

/

^일과

3

^회

/

^일^실 험구보다유의하게높았다

(P<0.05).

혈액성상

사료 공급 횟수에 따른 조피볼락의 혈액성상 분석 결과는

Table 3

에나타내었다

.

조피볼락 혈액내

GOT, GPT

그리고

hematocrit

^는^사료^공급^횟수에^영향을^받지^않았다

(P>0.05).

혈액내

GLU

^의^경우

0.5

^회

/

^일^실험구가

1

^회

/

^일

, 2

^회

/

^일^그리고

3

^회

/

^일^{실험구보다}^유의하게^높았다

(P<0.05).

혈액내

TCHO

와

HDLC

^는

1

^회

/

^일과

2

^회

/

^일^실험구가

0.5

^회

/

^일과

3

^회

/

^일^실험 구보다유의하게높은값을보였다

(P<0.05).

고 찰

사료공급횟수의증가는어류의성장과사료섭취율을높이지 만

,

^동시에^일정^횟수^이상의^사료^공급이^이루어질^경우^어류

Table 1. Initial and final weight, weight gain (WG), specific growth rate (SGR), daily feed intake (DFI), feed efficiency (FE) and survival of Korean rockfish Sebastes schlegelii fed at different feeding frequencies for 77 days

Treatment Initial weight Final weight WG SGR DFI FE Survival

(g/fish) (g/fish) (%) (%/day) (%/day) (%) (%)

0.5 meal/day 116.2±1.0 180.3±2.7â 57.9±0.7â 0.59±0.01â 0.75±0.02â 77.5±1.0^b 92.7±0.9 1 meal/day 116.7±0.8 197.1±0.2^b 68.9±0.9^b 0.67±0.01^b 0.84±0.01^b 77.9±1.3^b 93.7±2.3 2 meals/day 120.3±5.3 200.1±5.2^b 66.7±3.0^b 0.66±0.02^b 0.85±0.02^b 76.9±0.6^b 92.3±1.4 3 meals/day 116.6±2.6 181.9±1.0â 56.2±2.6â 0.58±0.02â 0.77±0.01â 71.8±1.4â 92.0±1.2 Values (mean±SE, n=3) with different superscripts in the same column are significantly different (P<0.05).

0 5 10 15 20 25

Coefficient variation (%)

Feeding frequency per day

3

1 2

0.5

Fig. 1. Coefficient of variation (mean±SE, n=3) in fish weight of Korean rockfish Sebastes schlegelii fed at different feeding frequencies for 77 days.

(4)

성장률에영향을미치지않거나감소하는연구결과들이많이 보고된바있다

(Seo and Lee, 2008; Lee et al., 2000; Küçük et al., 2013; Mizanur and Bai, 2014; Oh and Maran, 2015).

본연 구에서도일간

1

^회의^사료^공급^{횟수까지는}^증체율과^일간성장 률및사료섭취율이증가하였지만

,

더많은사료공급횟수

(

즉

, 2

^회

/

^일^또는

3

^회

/

^일

)

^에서는^성장률에^영향을^미치지^않거나^유 의한감소를보여최적의사료공급횟수는

1

^회

/

^일로^나타났다

.

Mizanur and Bai (2014)

^은

92-133 g

^의^{조피볼락을}^대상으로

1

회

/2

^일

, 1

^회

/

^일

, 2

^회

/

^일

, 3

^회

/

^일^그리고

4

^회

/

^일의^조건에서

1

^회

/

일에서가장높은증체율

,

일간성장률및사료섭취율을보였지 만

, 2

^회

/

^일^{이상에서는}

1

^회

/

^일와^성장^차이가^없거나^유의한^감 소를보여본실험과동일한결과를보였다

. Lee et al. (2000)

역 시어체중

6-20 g

^조피볼락^치어의^최적^사료^공급^횟수를

1

^회

/

일로보고하였다

.

^최적^사료^공급^횟수는^어종과^크기에^따라^달 Table 2. Whole body proximate composition (%, wet weight basis) of Korean rockfish Sebastes schlegelii fed at different feeding frequencies for 77 days

Treatment Moisture Crude protein Crude lipid Crude ash

Initial 68.32±0.13 17.36±0.08 7.68±0.03 4.93±0.04

0.5 meal/day 67.65±0.11^c 17.62±0.17â 10.24±0.13â 3.95±0.04â

1 meal/day 64.40±0.15^a 18.75±0.22^b 11.23±0.14^b 4.97±0.04^c

2 meals/day 64.49±0.16^a 18.23±0.17^b 11.18±0.13^b 4.91±0.03^c

3 meals/day 65.83±0.17^b 17.47±0.21^a 11.12±0.12^b 4.76±0.05^b

Values (mean±SE, n=3) with different superscripts in the same column are significantly different (P<0.05).

0.0 0.5 1.0 1.5

Food consumption (% body weight)

0.0 0.5 1.0 1.5

Time (h)

a a

b b

a

0.5 meal/d 1 meal/d

2 meals/d 3 meals/d

09:00 12:30 16:00 09:00 12:30 16:00

Fig. 2. Daily variation in food consumption at three feeding times of Korean rockfish Sebastes schlegelii fed at four different feeding frequencies. Values (mean±SE, n=3) with different letters are significantly different (P<0.05).

(5)

라진다

.

^예를^들어

, yellowtail flounder (Limanda ferruginea,

^초 기무게

6.8 g), hybrid sunfish (Lepomis cyanellus×L. macro- chirus,

초기무게

7.4 g)

^그리고

gibel carp (Carassius auratus gibelio,

초기무게

3.0 g)

의최적사료공급횟수는각각

2

회

/

일

(Dwyer et al., 2002), 3

^회

/

^일

(Wang et al., 1998)

^그리고

24

^회

/

^일

(Zhou et al., 2003)

^로^보고된^바^있다

.

^본^실험^결과^{사료효율은} 일간

2

회까지차이가없었지만일간

3

회에서는유의하게감소 하는결과를보였다

. Mizanur and Bai (2014)

^역시^동일^어종을 대상으로일간

3

회이상의사료공급횟수에서사료효율이유의 하게감소하는결과를보여본실험결과와유사하였다

.

^이것은 사료공급횟수의증가또는사료공급간격이짧아질경우섭 취한사료가체내소화관을거쳐가는시간이감소하고이에따 른효율적인소화가이루어지지않기때문이다

(Schnaittacher et al., 2005; Biswas et al., 2010; Mizanur and Bai, 2014).

그러 나

rock bream (Oplegnathus fasciatus)(Oh and Maran, 2015), mrigal (Cirrhinus mrigala)

^와

rohu (Labeo rohita)(Biswas et al., 2006)

그리고

flounder (Platichthys flesus luscus)(Küçük et

al., 2013)

^의^경우^사료^공급^횟수가^{사료효율에}^영향을^미치지

않는것으로나타나

,

어종및실험조건에따라달라지는것으 로나타났다

.

^본^실험^결과^사료^공급^횟수에^따른^개체간^크기 차이

(

^즉

, CV)

^는^나타나지^않아

,

^이전의

gibel carp (Zhou et al., 2003), white sturgeon (Acipenser transmontanus) (Cui et al., 1997)

^그리고

flounder (Küçük et al., 2013)

^와^유사한^결과를^나 타내었다

.

하지만

Oh et al. (2015)

과

Wang et al. (1998)

은각각

rock bream

^과

hybrid sunfish

^를^대상으로^사료^공급^횟수가^증 가할수록개체간크기차이를줄여일정한크기의어류생산을 할수있다고고찰하고있어어종에따라상이한결과를보였다

.

일간사료섭취패턴에대한정보는최적화된사료공급체 계를구축하는데유용한자료로활용될수있다

(Wang et al., 1998, Oh et al., 2015).

^본^실험^결과

09:00

^시와

16:00

^시에^높

은사료섭취가이루진반면

, 12:30

^시에는^사료^섭취가^유의하

게감소하는결과를보였다

.

이것은조피볼락이섭취하는사료 의소화를위해서는일정한시간이필요하며이에따른사료섭 취반응이사료공급시간간격에따라달라진것으로보인다

.

사료공급횟수에따른사료섭취비율은위의크기

(Ruohonen

and Grove, 1996),

^사료^공급^간격

(Liu and Liao, 1999)

^그리

고위배출속도

(Lee et al., 2000)

^등에^따라^달라진다

.

^향후^일 간사료섭취패턴에대한정보는조피볼락양식시하루중사 료공급시기및공급량결정을위한중요한정보를제공할수 있을것이다

.

사료공급횟수에따른체성분분석결과

1

^회

/

^일^실험구의^조 단백질과조지방함량이가장높고

3

^회

/

^일^{실험구에서}^유의한 감소또는감소하는경향을보였다

.

이것은이전의

92-133 g

범 위의조피볼락을대상으로한

Mizanur and Bai (2014)

^의^결과 와유사하였다

.

체내지방함량의경우빈번한사료공급에따른 여분의사료내에너지가어류의성장에사용되지않고체내지 방으로축적되며

, olive flounder (Lee and Pham, 2010)

^와

ayu (Cho et al., 2003)

등의어류에서동일한결과가보고된바있 다

. Xie et al. (2011)

^은^사료^공급^횟수^증가로^인한^충분한^사료

공급은사료를섭취하기위한경쟁과공식

(cannibalism)

을피

하기위한에너지를덜소모하기때문에체내에더높은함량의 단백질과지방을축적할수있다고고찰한바있다

.

^본^실험에 서

0.5

회

/

일실험구의단백질과지방함량이다른실험구에비 해낮게나타난것은제한된사료공급횟수로인한먹이경쟁과 공식을피하기위해더많은에너지의소모가하나의원인으로 작용할수있다

(Mizanur and Bai, 2014).

본실험결과조피볼락혈액내

hematocrit, GOT

^그리고

GPT

함량은사료공급횟수에대한영향이없었지만

, Mizanur and

Bai (2014)

^는^수온

15℃

^와

19℃

^두^조건에서^사료^공급^횟수가 증가할수록조피볼락혈액내

hematocrit, GOP

와

GPT

함량이 증가하는결과를보여본실험과차이를보였다

.

^하지만^이들은

2

^일에

1

^회^사료를^공급할^경우에도^먹이^부족에^따른^스트레스

반응을보인다는결과를볼때본실험에서나타난

0.5

회

/

일실

험구의

glucose

^함량^증가의^원인으로^작용한^것으로^생각된다

.

사료공급횟수는조피볼락혈액내

TCHO

와

HDLC

에영향을

미치는것으로나타났다

. Shimeno et al. (1997)

^은^사료^섭취율 이증가함에따라

commmon carp (Cyrinus carpio)

^의^콜레스 테롤함량이증가한다는보고를볼때가장높은사료섭취율 을보인

1

^회

/

^일와

2

^회

/

^일^{실험구에서}^동일한^현상이^나타난^것 으로생각된다

.

이상의결과에서

100-200 g

^범위의^조피볼락^성장률^향상을

위한최적의사료공급횟수는

1

^회

/

^일로^{나타났으며}

,

^향후^해상 Table 3. The concentration of GOT, GPT, glucose (GLU), total cholesterol (TCHO) and HDLC (high density of lipoprotein cholesterol) in the blood serum and hematocrit in the blood of Korean rockfish Sebastes schlegelii fed at different feeding frequencies for 77 days

Treatment Hematocrit GOT GPT GLU TCHO HDLC

% U/l mg/dl

0.5 meal/day 37.5±2.2 30.0±3.8 3.2±0.4 107.0±5.8^b 146.9±4.8^a 101.2±1.2^a

1 meal/day 40.1±1.2 44.0±8.8 3.8±0.9 44.1±4.6^a 171.9±4.9^b 105.1±1.3^b

2 meals/day 40.8±1.9 32.1±3.8 3.1±0.4 39.8±7.2^a 184.3±5.7^b 105.9±1.1^b

3 meals/day 39.6±2.3 43.3±7.7 4.4±0.7 28.1±3.0â 153.5±5.0â 100.0±0.9â

Values (mean±SE, n=30) with different superscripts in the same column are significantly different (P<0.05).

(6)

가두리내조피볼락양식을위한사료공급체계구축을위한기 초자료로활용할수있을것이다

.

사 사

본 연구는 한국해양과학기술진흥원 수산실용화기술개발 사업

“

^울릉도 ^해역에서 ^가두리를 ^활용한 ^{어류양식기술개발}

(PM59720)”

과한국에너지기술평가원산업기술혁신사업

“

해

상풍력단지해수공간을활용한산업융합설비개발및실증연 구

(20143010021800, PN66710)"

^의 ^연구비^지원으로 ^이루어 졌으며

,

^이에^감사를^드립니다

.

^실험기간^중^어류^사육과^관리

,

자료정리에도움을주신정유경연구원께도감사를드립니다

.

References

AOAC. 1990. Association of Official Analytical Chemists.

Official methods of analysis of the Association of Official Analytical Chemists, 15th edn. AOAC, Arlington, Virginia, U.S.A., 1298.

Biswas G, Jena JK, Singh SK, Patmajhi P and Muduli HK. 2006.

Effect of feeding frequency on growth, survival and feed utilization in mrigal, Cirrhinus mrigala, and rohu, Labeo

rohita, during nursery rearing. Aquaculture 254, 211–218.

http://dx.doi.org/10.1016/j.aquaculture.2005.08.001.

Biswas G, Thirunavukkarasu AR, Sundaray JK and Kailasam M. 2010. Optimization of feeding frequency of Asian sea- bass (Lates calcarifer) fry reared in net cages under brack- ishwater environment. Aquaculture 305, 26-31. http://

dx.doi.org/10.1016/j.aquaculture.2010.04.002.

Buurma BJ and Diana JS. 1994. Effects of feeding frequency and handling on growth and mortality of cultured walking catfish Clarias fuscus. J World Aquac Soc 175-182. http://

dx.doi.org/10.1111/j.1749-7345.1994.tb00179.x.

Cho SH, Lim YS, Lee JH, Lee JK, Park S and Lee S-M. 2003.

Effects of feeding rate and feeding frequency on survival, growth, and body composition of ayu post-larvae Pleco-

glossus altivelis. J World Aquac Soc 34, 85-91. http://dx.doi.

org/10.1111/j.1749-7345.2003.tb00042.x.

Cui Y, Hung SSO, Deng DF and Yang Y. 1997. Growth per- formance of juvenile white sturgeon as affected by feeding regimen. Prog Fish Cult 59, 31-35. http://dx.doi.

org/10.1577/1548-8640(1997)059<0031:GPOJWS>2.3.

CO;2.

Dwyer KS, Brown JA, Parrish C and Lall SP. 2002. Feeding frequency affects food consumption, feeding pattern and growth of juvenile yellowtail flounder (Limanda ferrugin-

ea). Aquaculture 213, 279-292. http://dx.doi.org/10.1016/

S0044-8486(02)00224-7.

Kubitz F and Lovshin LL. 1999. Formulated diets, feeding strategies, and cannibalism control during intensive culture of juvenile fishes. Rev Fish Sci 7, 1-22. http://dx.doi.

org/10.1080/10641269991319171.

Küçük E, Aydin İ, Polat H, Eroldoğan OT and Şahin T. 2013. Ef- fect of feeding frequency on growth, feed efficiency and nu- trient utilization of juvenile flounder (Platichthys flesusIus-

cus). Aquacult Int 22, 723-732. http://dx.doi.org/10.1007/

s10499-013-9701-2.

Lee S-M, Hwang U-G and Cho SH. 2000. Effects of feeding frequency and dietary moisture content on growth, body composition and gastric evacuation of juvenile Korean rock- fish (Sebastes schlegeli). Aquaculture 187, 399-409. http://

dx.doi.org/10.1016/S0044-8486(00)00318-5.

Lee, S-M and Pham MA. 2010. Effects of feeding frequency and feed type on the growth, feed utilization and body com- position of juvenile olive flounder (Paralichthys olivaceus).

Aquac Res 41, e166-e171. http://dx.doi.org/10.1111/j.1365- 2109.2010.02491.x.

Liu F-G and Liao CI. 1999. Effect of feeding regimen on the food consumption, growth and body composition in hybrid striped bass Morone saxatilis × M. chrysops. Fish Sci 64, 513-519. http://doi.org/10.2331/fishsci.65.513.

Lupatsch I, Kissil GW and Sklan D. 2003. Comparison of energy and protein efficiency among three fish species gilthead sea bream (Sparus aurata), European sea bass (Dicentrarchus

labrax) and white grouper (Epinephelus aeneus): energy ex-

penditure for protein and lipid deposition. Aquaculture 225, 75-189. http://dx.doi.org/10.1016/S0044-8486(03)00288-6.

Mizanur RM and Bai SC. 2014. The optimum feeding fre- quency in growing Korean rockfish (Sebastes schlegeli) rearing at the temperature of 15℃ and 19℃. Asian-Aus- tralas J Anim Sci 27, 1319-1327. http://dx.doi.org/10.5713/

ajas.2014.14193.

Oh S-Y and Maran BAV. 2015. Feeding frequency influences growth, feed consumption and body composition of juve- nile rock bream (Oplegnathus fasciatus). Aquacult Int 23, 175-184.

Ruohonen K and Grove DJ. 1996. Gastrointestinal responses of rainbow trout to dry pellet and low-fat herring diets. J Fish Biol 49, 501-513. http://dx.doi.org/10.1111/j.1095-8649.1996.

tb00045.x.

Okumus I and Bascinar N. 2001. The effects of different num- bers of feeding days on feed consumption and growth of rainbow trout [Oncorhynchus mykiss (Walbaum)].

Aquac Res 32, 365-367. http://dx.doi.org/10.1046/j.1365- 2109.2001.00566.x.

Schnaittacher G, William KV and Berlinsky DL. 2005. The effects of feeding frequency on growth of juvenile Atlantic halibut, Hippoglossus hippoglossus L. Aquac Res 36, 370- 377. http://dx.doi.org/10.1111/j.1365-2109.2005.01218.x.

Seo J-Y and Lee S-M. 2008. Effects of dietary macronutrient level and feeding frequency on growth and body composi- tion of juvenile rockfish (Sebastes schlegeli). Aquacult Int 16, 551-560. http://dx.doi.org/10.1007/s10499-008-9165-y.

(7)

Shimeno S, Shikata T, Hosokawa H, Masumoto T and Kheyyali D. 1997. Metabolic responses to feeding rates in common carp, Cyprinus carpio. Aquaculture 151, 371-377. http://

dx.doi.org/10.1016/S0044-8486(96)01492-5.

Sveier H and Lied E. 1998. The effects of feeding regime on growth, feed utilization and weight dispersion in large Atlantic salmon (Salmo salar) reared in seawater. Aqua- culture 165, 333-345. http://dx.doi.org/10.1016/S0044- 8486(98)00269-5.

Wang N, Hayward RS and Noltie DB. 1998. Effect of feeding frequency on food consumption, growth, size variation, and feeding pattern of age-0 hybrid sunfish. Aquaculture 165, 261-267. http://dx.doi.org/10.1016/S0044-8486(98)00266- Wang Y, Kong L, Li K and Bureau DP. 2007. Effects of feeding X.

frequency and ration level on growth, feed utilization and ni- trogen waste output of cuneate drum (Nibea miichthioides) reared in net pens. Aquaculture 271, 350-356. http://dx.doi.

org/10.1016/j.aquaculture.2007.03.022.

Xie F, Ai Q, Mai K, Xu W and Ma H. 2011. The optimal feeding frequency of large yellow croaker (Pseudosciaena crocea, Richardson) larvae. Aquaculture 311, 162-167. http://dx.doi.

org/10.1016/j.aquaculture.2010.12.005.

Zhou Z, Cui Y, Xie S, Zhu X, Lei W, Xue M and Yang Y. 2003.

Effect of feeding frequency on growth, feed utilization, and size variation of juvenile gibel carp (Carassius aura-

tus gibelio). J Appl Ichthyol 19, 244-249. http://dx.doi.

org/10.1046/j.1439-0426.2003.00453.x.