Determination of Fertilizer Application Amount for Cnidium officinale Makino Cultivated in Silty Loam Soil Located at Alluvial Fan Using a Response Surface methodology

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351 -P5-8

Determination of Fertilizer Application Amount for Cnidium officinale Makino Cultivated in Silty Loam Soil Located at Alluvial Fan Using a Response

Surface methodology

1_{Bonghwa Alpine Medicinal Plant Research Station, Gyeongbuk A.T.A.}

2_{Dep. of Herbal Crop Research, National Institute of Horticulture and Herbal Science:}

Young-Jin Seo1_{, Jae-Cheol Kim}1_{, Won-Cheol Jang}1_{, Hyong-Rac Sohn}1_{, Jong-Phil Lee}1_,

Min-Ki Kim1, Chun-Geun Park2, Young-Sup Ahn2

선상지에 분포하는 미사질양토에서 반응표면분석을 활용한 일천궁 시비량 결정

1_{경북농업기술원 봉화고냉지약초시험장 : 서영진}*_{, 김재철, 장원철, 손형락, 이종필, 김민기}

2_{국립원예특작과학원 인삼특작부 : 박춘근, 안영섭}

Objectives

The aim of this study was conducted to determine the optimal levels of fertilizer application amount such as nitrogen, phosphorous and potassium etc., for Cnidium officinale Makino cultivated in silty loam soil located at alluvial fan.

Materials and Methods

The study was carried out in Bonghwa Alphine Plant research Station. Soil was classified as haenggok series (loamy skeletal, mixed, mesic family of Aquic Udifluvents). This soil locate on Alluvial fan have shallow, dark grayish colored Ap horizon . The experimental soil was silty loam, with pH, organic matter, available phosphate, exchangeable K, Ca and Mg of 6.7, 22.8 g kg-1_{, 358 mg kg}-1_{, 0.78 cmol}

c kg-1, 5.07 cmolc

kg-1 and 1.95 cmolc kg-1 respectively. Urea, fused phosphate and potassium chloride were

used a N, P, and K fertilizer. five application concentration of N, P and K fertilizer were treated with central composite design and analyzed through a response surface methodology based on root yield of Cnidium officinale Makino.

Results

The coefficiencts of determination (R2) and coefficient variation (CV) of response surface regression equation for the optimal amounts of NPK fertilization during in cultivating a Cnidium officinale Makino. were 0.856 and 11.1175. As a result, the optimal application amount of NPK were determined to be 12 (N), 10 (P) and 15 (K), Rrespectively. Content and uptake amount of nitrogen in root showed a positve relationship with appliction rate of nitrogen.

Corresponding author : 서영진 E-mail : [email protected] Tel : 054-673-8064

사사: 본 연구는 농촌진흥청 어젠다공동연구사업 주요 약용작물 고품질 종자생산 확대기 술 개발과제(PJ90693822)의 연구비 지원에 의하여 수행되었음.

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352

-pH(1:5) _{(g kg}OM-1₎ Avail.P_{(mg kg}2-1O₎5 Exch. Cation (cmolc kg -1₎

K Ca Mg

6.7±0.25 22.8±0.25 358±30.3 0.78±0.38 5.07±0.49 1.95±0.32

No. Independent variables Yield (kg 10a-1₎

N P K 1 0 18 24 350.1 2 5 9 12 397.2 3 5 9 36 369.2 4 5 27 12 356.0 5 5 27 36 344.1 6 10 0 24 382.1 7 10 18 0 388.5 8 10 18 24 389.9 9 10 18 48 326.0 10 10 36 24 330.1 11 15 9 12 399.1 12 15 9 36 367.8 13 15 27 12 358.1 14 15 27 36 330.1 15 20 18 24 350.3 Result of RSM Amount of fertilizer calculated RSM (kg 10a-1₎ N P K Y=330.52+9.9913×N+2.3296×P+1.8836×K– 0.4233×N2_{–0.0343×NP– 0.1181×P}2_{–0.0406×PK–} 0.0635×K2 (R2 = 0.856, CV=11.1175) 12 10 15

Table 1. Soil chemical properties before experiment (n=15).

Table 2. Levels of independent variables for central composition design and yield of

Cnidium officinale Makino.

Table 3. Polynominal equation caculated by response surface methodology (RSM) and optimal levels of fertilizer for Cnidium officinale Makino.