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Wnt/β-catenin signaling promotes midbrain specification of neural precursor cells derived from human embryonic stem cells

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Academic year: 2021

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Figure 2. Inhibition  of GSK-3 increases midbrain characteristics (A)  The structure  of  BIO  (B,  C)  Dose-response  and  cytotoxicity  experiments  in  H9  NPCs  revealed  that 1 μM BIO was the optimal dose to increase En1 expression
Figure  3.  Wnt/β-catenin  signaling  pathway  can  modulate  expression  of  En1  (A)  Treatment with 20 ng/ml Wnt1 increased En1 expression after 7 days in NPCs, as  assessed  by  quantitative  RT-PCR
Figure  5.  Generation  of  dopaminergic  neuron  (A)  Immunocytochemistry  of  TH + /Tuj1 +   neurons  after  maturation  into  DA  neuron  from  NPCs  with  or  without  BIO  treatment  derived  from  H9  human  ES  cells
Figure 6. The comparison between no-treat control group and BIO-treated group of  NPCs  after  DA  differentiation  by  immunostaining  (A)  Coexpression  of  TH  (red)  and  En1  (green)  was  observed  in  BIO-treated  group

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