Effect of the Water Extracts of Codonopsis lanceolata on the Growth of Rat Pheochromocytoma Nerve Cells

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257 -P3-46 더덕 추출물이 쥐의 신경 세포 생장에 미치는 효과 강원대학교 생물소재공학과 : 송치호, 최운용, 김지선, 이춘근, 서용창, 마충제, 이현용5) 농촌진흥청 국립농업과학원 농식품자원부 기능성식품과 : 박동식 뉴트리 : 정재윤, 김도언, 정희철

1_{Department of Biomaterials Engineering, Kangwon National University, Chuncheon} 200-701, Korea

2_{Functional Food & Nutrition Division, Rural Development Administration, Gyeonggi} 441-711, South Korea

3_{NewTree CO., Ltd, 11F Tech center, SK n Techno Prak, Sungnam 462-120, Korea}

Chi-Ho Song1_{, Woon-Yong Choi}1_{, Ji-Seon Kim}1_{, Choon-Geun Lee}1_{, Yong-Chang Seo}1_,

Dong-Sik Park2, Jae-Youn Chung3, Do-Un Kim3, Hee-Chul Chung3, Choong-Je Ma1, and Hyeon-Yong Lee1*

실험목적 (Objectives)

The study investigated enhancement of cognitive function by adding hot steamed

Codonopsis lanceolata.

재료 및 방법 (Materials and Methods)

Both fresh Codonopsis lanceolata (FC) and hot steamed Codonopsis lanceolata (SC) were extracted by water at 100 ℃for 24 h to compare the growth of rat nerve cell, PC12 for screening the improvement of cognitive function.

실험결과 (Results)

Growth of PC12 cells was enhanced up to 5×102 cells/mL in adding 60 μg/mL of SC for 5 days cultivation while 2.5×104 cells/mL FC extracts, compare to 1.2×104 cells/mL of the control. Moreover, the numbers of neurite bearing cell was increased up to 20-25%, compare to the case of adding FC extracts. It was found that the C. lanceolata can enhance both the proliferation and differentiationof nerve cell, result in enhancing the cognitive function. The steaming process can alone greatly help to improve its activities by extracting of large amounts of biologically active compounds such as compound of lancemaside.

주저자 연락처 (Corresponding author) : 이현용 E-mail : [email protected] Tel : 033-256-4819

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-Fig. 1. The growth of PC12 cells in adding 60 ㎍/mL of the extracts of

C. lanceolata for 5days.

Fig. 2. Effect of C. lanceolata on differentiation of PC12 cells in adding 60 ㎍/mL of C. lanceolata