Enhancement of Antioxidant Activities of Curcuma longa Linne leaves by Ultra High Pressure Extraction

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267 -P3-51

초고압 공정을 이용한 Curcuma longa Linne 추출물의 항산화 활성 증진

강원대학교 생물소재공학과 : 김지선, 서용창, 최운용, 이춘근, 송치호, 이현용10)

세바바이오텍 : 임혜원 전라북도 농업기술원 : 안민실

Enhancement of Antioxidant Activities of Curcuma longa Linne leaves by Ultra High Pressure Extraction

1_{Department of Biomaterials Engineering, Kangwon National University, Chuncheon} 200-701, Korea

2_{Shebah Biotech Co., Chuncheon Bioindustry Foundation Bio-1 #412, Chuncheon} 200-702, Korea

3_{Jeonbuk Provincial ARES, Iksan 570-704, Korea}

Ji Seon Kim1, Yong Chang Seo1, Woon Yong Choi1, Choon Geun Lee1, Chi Ho Song1, Hye Won Lim2, Min Sil Ahn3, Hyeon Yong Lee1*

Objectives

This study, we were to enhance antioxidant activities of Curcuma longa Linne leaves extracts by ultra high pressure extraction process

Materials and Methods ◦ Materials

Curcuma longa Linne leaves was extracted by water extraction at 100℃ and 60℃,

70% ethyl alcohol extraction at 60℃ and ultra high pressure extraction at 500 ㎫ for 30 minutes at 60℃.

◦ Methods

In order to measured antioxidant activities, we measured total phenol and flavonoids contents, DPPH free radical scavenging activity and reducing power.

Results

Total phenolic acid contents from WE, EE and HPE were show as 12.28, 11.36 and 21.82 µg/ml, respectively. The flavonoids contents were measured as 14.44, 15.49 and 26.11 µg/ml, respectively. The extracts from ultra high pressure extraction showed the strongest free radical scavenging activities 72.3% in adding 1 mg/ml of sample, compared to other extraction processes from WE and EE as 64.05 and 62.55%, respectively. The reducing power of the extracts for WE, EE and HPE were estimated as 52.89, 61.87 and 67.54%, respectively in adding 1 mg/ml. Antioxidant activities of Curcuma longa Linne leaves from ultra high pressure extraction process 주저자 연락처 (Corresponding author) : 이현용 E-mail : [email protected] Tel :

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-would be caused by the releases large amounts of active contents such as polyphenol compound and flavonoids that could play an important role in antioxidant activity.

Fig. 1. Total flavonoids and phenolic acid contents of the extracts of

Curcuma longa Linne leaves by different extraction processes and

‡_{WE: water extraction at 100℃; EE: 70%}

ethyl alcohol extraction at 60℃; HPE: high pressure extraction .0for 30 minutes at 6 0℃ with 70% ethyl alcohol solvent.

Fig. 3. Reducing power of the extracts of

Curcuma longa Linne leaves by different extraction processes andconcentration.

‡_{See the Fig. 1. for abbreviation.}

Fig. 2. DPPH free radical scavenging activities of the extracts of

Curcuma longa Linne leaves by different extraction processes and concentration.