Quantitative Analysis of Microcystins, Cyanobacterial Toxins in Soyang Lake ,

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Printed in the Republic of Korea

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*

(2002. 9. 13 )

Quantitative Analysis of Microcystins, Cyanobacterial Toxins in Soyang Lake

Jungae Lee, Soyoung Lee, and Dongjin Pyo*

Department of chemistry, Kangwon National University, Chuncheon 200-701, Korea (Received September 13, 2002)

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ABSTRACT It is very difficult to analyze the microcystins, cyanobacterial toxins quantitatively since it exists in a trace level in lakes. In this paper, two different analytical methods were tried to analyze the microcystins, cyanobacterial toxins quantitatively in water samples collected in Soyang lake. The first method was solid phase extraction method fol- lowed by High Performance Liquid Chromatography(HPLC), and the second method was Enzyme-Linked Immu- nosorbent Assay(ELISA) using the monoclonal antibody of microcystin.

Keywords: Cyanobacterial toxin, Microcystin, Quantitative analysis

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Sample HPLC (pg/mL) ELISA (pg/mL)

March 12th 2002 93.3 100

April 8th 2002 111.7 150.8

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June 19th 2002 241.7 257.7

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Fig. 4. Chromatograms of each month in Soyang Lake (2002) (A) March 12th, (B) April 8th, (C) May 13th, (D) June 19th, (E) July 12th, (F) August 20 th.

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Fig. 5. Calibration curve of ELISA.

Fig. 6. Correlation between HPLC and ELISA.

Quantitative Analysis of Microcystins, Cyanobacterial Toxins in Soyang Lake ,  

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Quantitative Analysis of Microcystins, Cyanobacterial Toxins in Soyang Lake

Quantitative Analysis of Microcystins, Cyanobacterial Toxins in Soyang Lake ,

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