The effect of β -sitosterol proliferation and apoptosis
in human uterine leiomyoma cells Youngsun Park, Seunghee Baek
Dept. of gynecology, College of Orinental Medicine, Daegu Haany University
β
β
β
β μ
β μ
β
β
대구한의대학교 한의과대학 부인과 교실 박영선 백승희,
β β
β
β μ
β
β
μ
β μ
μ
β μ
β μ
β
β μ
0 0 . 2 0 . 4 0 . 6 0 . 8 1 1 . 2
T im e a f t e r t r e a t m e n t o f β - s it o s t e r o l ( h r )
Cell number (ratio)
0 2 4 4 8 7 2
*
0 0 . 2 0 . 4 0 . 6 0 . 8 1 1 . 2
T im e a f t e r t r e a t m e n t o f β - s it o s t e r o l ( h r )
Cell number (ratio)
0 2 4 4 8 7 2
*
β μ
β
β μ
0 0 .2 0 .4 0 .6 0 .8 1 1 .2
T im e a fte r tre a tm e n t o f β - s ito s te ro l (h r)
Cell number (ratio)
0 2 4 4 8 7 2
*
0 0 .2 0 .4 0 .6 0 .8 1 1 .2
T im e a fte r tre a tm e n t o f β - s ito s te ro l (h r)
Cell number (ratio)
0 2 4 4 8 7 2
*
β μ
β μ
β μ
Control
Control 24hr 24hr
48hr
48hr 72 hr72 hr
6 3 .2 2 4 .8 4 1 5 .0 0 1 6 .9 7 7 2 h r
6 8 .2 4 6 7 .0 1
6 8 .3 6 G 2 /M
5 .1 0 6 .7 7
6 .2 0 S
2 1 .7 2 2 2 .5 1
2 2 .8 4 G 1
4 .7 8 3 .1 1
1 .9 0 S u b G 1
4 8 h r 2 4 h r
C o n tr o l
6 3 .2 2 4 .8 4 1 5 .0 0 1 6 .9 7 7 2 h r
6 8 .2 4 6 7 .0 1
6 8 .3 6 G 2 /M
5 .1 0 6 .7 7
6 .2 0 S
2 1 .7 2 2 2 .5 1
2 2 .8 4 G 1
4 .7 8 3 .1 1
1 .9 0 S u b G 1
4 8 h r 2 4 h r
C o n tr o l
β μ
β μ
β μ
0 0 . 1 0 . 2 0 . 3 0 . 4 0 . 5 0 . 6 0 . 7
DNA Fragments (Relative Unit)
0 2 4 4 8 7 2 T im e a fte r tre a tm e n t o f β - s ito ste ro l ( h r)
*
0 0 . 1 0 . 2 0 . 3 0 . 4 0 . 5 0 . 6 0 . 7
DNA Fragments (Relative Unit)
0 2 4 4 8 7 2 T im e a fte r tre a tm e n t o f β - s ito ste ro l ( h r)
*
β
μ
β μ
β- t u b u l in ( 4 6 k D a ) P 2 1 ( 2 1 k D a ) P 2 7 ( 2 7 k D a )
C o n tr o l 2 4 4 8 7 2
C D K 2
C D K 4 C y c li n E
S k p 2 p 4 5 T i m e ( h r ) :
β - s i t o s t e r o l
β- t u b u l in ( 4 6 k D a ) P 2 1 ( 2 1 k D a ) P 2 7 ( 2 7 k D a )
C o n tr o l 2 4 4 8 7 2
C D K 2
C D K 4 C y c li n E
S k p 2 p 4 5 T i m e ( h r ) :
β - s i t o s t e r o l
β
μ β μ
β
β μ
Procaspase 3 (32kDa)
C ontrol 24 48 72
Rb (116kDa) PARP (116/85kDa)
E2F-1 β-tubulin (46kDa)
116kD a 85kDa Tim e (hr) :
β-sitosterol
Procaspase 3 (32kDa)
C ontrol 24 48 72
Rb (116kDa) PARP (116/85kDa)
E2F-1 β-tubulin (46kDa)
116kD a 85kDa Tim e (hr) :
β-sitosterol
