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The Effect of the Crude Extracts from sp. on Antiradical and Antioxidation

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90 2006년 한국생명과학회 추계학술대회

P115

The Effect of the Crude Extracts from Alnus sp.

on Antiradical and Antioxidation

Hye Jung Choi, Min A Lee, Jeong Uck Park1, Ja Young Moon2, Dong Wan Kim3, Young Whan Choi4, Young Guen Lee5, Young Kee Jeong6 and Woo Hong Joo*

Interdisciplinary Program in Biotechnology, 1Department of Biology, 2Department of Biochemistry and Health Sciences, 3Department of Microbiology, Changwon National University, 4School of Bioresource Science, 5School of Applied Life Science, Busan National University, 6Faculty of Natural Resources and

Life Sience, Dong-a University

In the course of research on the bioactive constituents of woody plants from the Goseong area of Korea, a methanol extract of the Alnus. sp were found to show strong antiradical and antioxidant activity. The crude extracts of the Alnus. sp were assessed using different antioxidant tests: DPPH ac- tivity, β-carotene linoleate model system, ferric thiocyanate (FTC) effect, reducing power and metal chelating activities in vitro. As a result, we found that the ethyl acetate- soluble fractions from the methanol extract had a potent antioxidant activity. In addition, a fraction was purified by silica gel col- umn chromatography and preparative HPLC.

P116

Isolation and application of insoluble phosphate solubilizing strains

Soo-Jung Kim, Ji-Hyun Park, Hyo Eun Jeon, Min Hee Kim and Hyun-Soo Kim

Department of Microbiology, College of Natural Science, Keimyung University, Taegu 704-701, Korea

Strain L-1 and BR-1 that solubilized insoluble phosphate were isolated from soil. The maximum growth conditions were investigated about carbon and nitrogen sources. Strain L-1 showed the excellant growth in the culture broth with glucose and peptone addition. Strain BR-1 revealed the excellant growth under the addition of glucose and yeast extract. In the result of garden plant experiment using two strains, the cabbage field showed the high phosphate solubilizing activity when strain L-1 and BR-1 culture broth sprayed.

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