경락경혈학회지 Vol.27, No.4, pp.15 23, 2010∼ Korean Journal of Acupuncture
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Tae-Yeon Choi , Sung-Won Lee , Yeon-Hee Ryu , Hyo-Jeong Ban , Geun-Young Seo , Jae-Hyo Kim , Seong-Hun Ahn , In-Chul Sohn Dept. Of Meridian & Acupoint, College of Oriental Medicine, Wonkwang University Division of Standard Research, Acupuncture, Moxibustion and Meridian Research Center,
Korea Institute of Oriental Medicine, Daejeon, Korea
Research Center of Korean Traditional Medicine, Wonkwang University
Abstract
Objectives : In order to confirm the anti-cancer effect of Trichosanthes kirilowii pharmacopuncture fluid, this study was proceeded.
Methods : A549 lung cancer cells were cultured to be treated by Trichosanthes kirilowii pharmacopuncture fluid as dose dependent manner for 72 hours. And then the cell viability, nucleus fragmentaion, p21 and p53 protein expression, Bcl-2 and Bax protein expression, procaspase-3 PARP protein expression.
Results :
1. Trichosanthes kirilowii pharmacopuncture fluid decrease A549 cell viability as dose dependent manner.
2. Trichosanthes kirilowii pharmacopuncture fluid induced the nucleus fragmentation in A549 lung cancer cells as dose dependent manner.
3. Trichosanthes kirilowii pharmacopuncture fluid increase the p21 and p53 protein expression.
4. Trichosanthes kirilowii pharmacopuncture fluid decrease the Bcl-2 protein expression but cannot affect the Bax protein expression.
5. Trichosanthes kirilowii pharmacopuncture fluid increase the activation of caspase-3 and PARP protein.
Conclusions : As the above results, it was conclused theTrichosanthes kirilowii pharmacopuncture fluid had the anti-cancer effects to induce apoptosis.
Key words : Trichosanthes kirilowii, pharmacopuncture, A549, lung cancer, apoptosis
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Fig. 1. Effects of Trichosanthes kirilowii phamacopuncture fluid on the cell viability in A549 cells.
Fig. 2. Effects of Trichosanthes kirilowii phamacopuncture fluid on the nucleus fragmentation of A549 cells.
- 19 - Fig. 3. Effects on Trichosanthes kirilowii phamacopuncture fluid on the expression of p53 and p21 protein in A549 cells.
Fig. 4. Effects on Trichosanthes kirilowii phamacopuncture fluid on the expression of Bcl-2 protein in A549 cells.
Fig. 5. Effects on Trichosanthes kirilowii phamacopuncture fluid on the expression of procaspage-3 protein in A549 cells.
- 21 - Fig. 6. Effects on Trichosanthes kirilowii phamacopuncture fluid on the expression of PARP and C-PARP protein in A549 cells.
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