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Effects of Yukmigeehwang-hwan Pharmacopuncture Extracts on the Collagenase Activity and Procollagen Synthesis in HS68 Human Fibroblasts and Tyrosinase Activity

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Academic year: 2021

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이세나 김명규, ,김명희,김형준,조학준,김이화,임강현 ,

Abstract

목적 : 본 연구는 六味地黃丸 藥鍼液가 사람 피부 섬유아세포의 콜라게나제 활성 및 프로 콜라겐 합성에 미치는 영항과 티로시나제 활성에 미치는 효과를 측정하고자 실시하였다.

방법 : HS68 사람 정상 섬유아세포에 UVB조사 후 六味地黃丸 藥鍼液가 type I procollagen 생성과 콜라게 나제 효소활성에 미치는 효능과 티로시나제 효소활성에 미치는 효능을 평가하였다.

결과 : 六味地黃丸 藥鍼液은 UVB 조사된 세포의 콜라게나제 효소활성을 통계적으로 유의하게 억제하였고 티로, 시나제 활성과 L-DOPA oxidation 활성 또한 통계적으로 유의하게 억제하였다. 그러나 티로시나제와

억제활성의 정도는 미약하였다

L-DOPA oxidation .

결론 : 六味地黃丸 藥鍼液의 콜라게나제 억제효능은 주름개선 약침 치료에 활용이 가능할 것으로 생각된다.

Key words : Yukmigeehwang-hwan, Liuweidihuang-wan, Type I procollagen, Collagenase, Tyrosinase

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Fig. 1. Cell viability of Yukmigeehwang-hwan pharmacopuncture extracts (YPE) on HS68 human fibroblasts.

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Fig. 2. Effect of Yukmigeehwang-hwan pharmacopuncture extracts (YPE) on collagen type I synthesis in human fibroblast cells.

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Fig. 4. Effect of Yukmigeehwang-hwan pharmacopuncture

extracts (YPE) on tyrosinase activity.

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Fig. 5. Effect of Yukmigeehwang-hwan pharmacopuncture

extracts (YPE) on L-DOPA oxidation.

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수치

Fig. 1. Cell viability of Yukmigeehwang-hwan pharmacopuncture extracts (YPE) on HS68 human fibroblasts.
Fig. 2. Effect of Yukmigeehwang-hwan pharmacopuncture extracts (YPE) on collagen type I synthesis in human fibroblast cells.
Fig. 5. Effect of Yukmigeehwang-hwan pharmacopuncture extracts (YPE) on L-DOPA oxidation.

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