Antimicrobial Activity of Streptococcus mutans by Herbal Medicine Extracts

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(1)Journal of Dental Hygiene Science Vol. 2, No. 1, pp. 0~0 (2002). Streptococcus mutans

(2) . . †. 1

(3) . . 1. 2. Antimicrobial Activity of Streptococcus mutans by Herbal Medicine Extracts †. Eun-Sook Jeun , Su-Hong Yoon and Man-Deuk Han Dept. of Dental Hygiene, Pohang College, Pohang city 791-711, Korea Dept. of Pharmacy, Catholic University of Taegue, Taegue city 712-702, Korea 2 Dept. of Dental Hygiene, Kimcheon College, Gimcheon, Gyeongbuk 740-704, Korea 1. . This study was to investigate the antibacterial effect of 63 species herbal medicine extracts on S. mutans growth by paper disc method and then examined the effects of S. mutans growth by some selected herbal medicines. Antibacterial effect of 63 species medicinal herb extracts on S. mutans was superior to Lycopi herba, Scutellariae radix in regular order and antibacterial activity(20 mg/ml concentration) through paper disc method were measured 6.3 mm and 3.5 mm, respectively. The growth of S. mutans in control medium was the highest at 8hrs, while that of medicinal herbs extract added-medium(2 mg/ml) was at 16 hrs. The pH values of the control media and media supplemented with Lycopi herba and Scutellariae radix extract were 5.08, 5.80, and 5.74 at 8 hrs, respectively. In the change of total carbohydrate of the control media and supplemented with Lycopi herba and Scutellariae radix extract were 0.81 mg/ml, 1.70 mg/ml and 1.84 mg/ml at 8 hrs, respectively. In the change of proteins of the control media and supplemented with Lycopi herba and Scutellariae radix extract were 8.39 mg/ml, 9.52 mg/ml and 9.28 mg/ml at 8 hrs, respectively. The polysaccharide contents of the control media and supplemented with Lycopi herba and Scutellariae radix extract were 300 mg/100 ml, 200 mg/ 100 ml and 220 mg/100 ml at 8hrs, respectively..

(4) . Streptococcus mutans, Herbal medicine extracts, Antimicrobial activity. . S. mutans2 , @;-. G U?@A VO /W= A >M3 XY A Z[\] A /0 . ^_5 àA bO52 ! A cd-Be, fgh", ! :;12 iA cdj P k2 lm>%d d

(5) nT. op, A à-W b Wq r s l m>% A VO 0> cd, r

(6) tu vwtx k9, y 4,56 zr%A + m>% cd, O {| }t5 , r

(7) ~-S vwtx k. zr% : , l I?, { O52 magnolol? hinokiol , gymnemic acid , polyphenol , s= % ? cardinol x1x k. #,56 à < 4A .

(8) t > % , {-. r1x kx, N N%(Grapefruit seed extract) ascorbic acid, O S. mutans , l!*+A e&x x1x k . op :*+ A àj P k2 a 2,3).

(9) (progressively infectious disease). !" # !, $%, &' ()*+, -. /012 3 !" # ! 4, 56 Streptococcus mutans

(10) 7 8!9, S. mutans2 , :;, < = > ?@A BC A /0 . D, S. mutans2 EF GH >-2 glucosyltransferase (GTF) IJ KLM3 NG(sucrose)A KO-. P+ Q2 LP+ glucanA >-9, 4 LP+ glucan , :;-. !"A R-S T. , :; 1). 4). 9). 5). 6-8). 10). 11,12). †. Corresponding author Tel: 054-245-1222 Fax: E-mail: [email protected]. 13). .

(11) . Antimicrobial Activity of Streptococcus mutans by Herbal Medicine Extracts. X, {| 415 zr%,5 l% A K-xN -2 \61x k2 \,, %,5 zr l !% $P, ,

(12) d < ~' +1w A \ P k y 2 ¡ ¢x -£. , r,52 #,5 /V12 63 ¤: ¥P%A K ¦, S. mutans , l!I? paper disc method §¨-x, l!I?

(13) e&e2 ¤ + -. S. mutans U, m2 ©ªA «W b-. > ¬ , pH ®¯, ° ®¯, ± ²P¯% ®¯ < GH > , m2 I? , {-. ³´-xN -µ. 1.. . . ¶·, U+ ¤(t:N F 62)2 5¸ ¹\ ,5 º(» iA ¼-. U+-µ9, Table 1 ,5 2 ½¾ ¿.. aX ÀÀ ¤ Á ¢W KÂ-. 50 g Ã ÄÅ ¦ ÆÀÇ_È¢, Éx \

(14) ÊW6Ë HP 1 l

(15) $ 100 C ,5 HÌÀW +-. PÍ(,5 3\Î Ï -µ. .?\] .?'A Ðx ÑU2 \ Ò.? ?@A BC .'A Ð ¦ .'A ÓO [ÔÕÖ W U+-. × º\Ø ¦ \ ºd-µ. ¤ U+12 :b, ^_ \Ù Ú , ¥¡, Û, Ü A ( -µ(Scheme 1). (2) \ < WW ¶·, U+ \2 BCA protein assay kit(Piece Co., U.S.A), BSA(Piece Co., USA)A U+Ý9, S. mutans Þ~A b Þt2 brain heart infusion(Difco, USA)A U+µ. WW2 pH meter(Istek Co., Korea), UV/Vis spectrophotometer(Shimadzu Co., Japan), centrifuge(Hanil Co., Korea), ßWN(BBL Co.), incubator(Dongyang Co., Korea), clean bench 2.. (1). o. (Sangwoo Co., Korea), voltex(Dongyang Co., Korea), magnetic. List of tested samples Serial No.. Korean name. Name of herbal medicines. Serial No.. Korean name. Name of herbal medicines. DH-01 DH-02 DH-03 DH-04 DH-05 DH-06 DH-07 DH-08 DH-09 DH-10 DH-11 DH-12 DH-13 DH-14 DH-15 DH-16 DH-17 DH-18 DH-19 DH-20 DH-21 DH-22 DH-23 DH-24 DH-25 DH-26 DH-27 DH-28 DH-29 DH-30 DH-31 DH-32. Goihwa Gilkyung Duchung Soksae Ogapi Usul Doin Haedongpi Cheonma Pogongyung Mokdanpi Goldamcho Ganghwal Mogun Hwanggum Dansam Baekdugu Bokbunja Gamguk Golyonpi Bija Jibuja Baekdanhang Daepungja Gumaengja Binlangja Ganghwang Tosaja Baeklyom Baeksunpi Baekgulchae Gosam. Sophorae flos Platycodi radix Eucommiae cortex Equiseti herba Acanthopanacis cortex Achyranthis radix Persicae semen Kalopanacis cortex Gastrodiae rhizoma Toraxaci herba Moutan cortex radix Caraganae radix Angelicae koreanae radix Imperata rhizoma Scutellariae radix Salviae radix Amomi cardamomi fructus Rubi fructus Chrysanthemi flos Meliae cortex Torreyae semen Lycopi herba Santali album lignum Hydnocarpi semen `Rosae laevigatae fructus Arecae semen Curcumae longae rhizoma Cuscutae semen Ampelopsis radix Dictamni radicis cortex Chelidonii herba Sophorae radix. DH-33 DH-34 DH-35 DH-36 DH-37 DH-38 DH-39 DH-40 DH-41 DH-42 DH-43 DH-44 DH-45 DH-46 DH-47 DH-48 DH-49 DH-50 DH-51 DH-52 DH-53 DH-54 DH-55 DH-56 DH-57 DH-58 DH-59 DH-60 DH-61 DH-62 DH-63. Guchuk Sansaja Sanyak Sandugun Sukchangpo Choyongdam Sokdan Sungma Siho Sini Gongsain Yonkyo Aeyop Chodugu Pohwang Indong Jeonho Gungang Eumyangguk Jogudong Jinbum Magamok Mahwang Jinpi Daehwang Chuncho Chunlunja Chungsangja Chukbaeyop Donggoaja Danggui. Cibotii rhizoma Crataegi fructus Dioscoreae rhizoma Sophorae subprostratae radix Acori graminei rhizoma Labiatae flos Phlomidis radix Cimicifugae rhizoma Bupleuri radix Magnoliae flos amomi xanthioidis fructus Forsythiae fructus Artemisiae asiaticae herba Katsumadai alpiniae semen Typhae pollen Lonicerae flos Anthrisci radix Zingiberis rhizoma Epimedii herba Ramulus uncariae cum uncis Aconitum flos Sorbus cortex Ephedrae herba Citrus unshin peel Rhei Rhizoma Zanthoxyli pericarpium Meliae fructus Caricae fici fructus Meliae flos Benincasae semen Angelicae gigantis radix.

(16) . Journal of Dental Hygiene Science Vol. 2, No. 1, pp. 0~0 (2002). ç!T Muller hinton agar Þt petri dish, 15 ml Ã K7-. óx\Ø ¦, S. mutans Þ~' 50 µl æ-. !s-S 6è-µ. ¤ % oq Õ6 10 mg/ml 2Þ ru ôõ ¦ 37 C ,5 24\Î Ï ßW Þ~. öÏ {÷-. T Õ6¾ cdT Õ6 ø@µ. (6) > ¬ å@ > ¬ S. mutans !7 1 loop 10 ml BHI broth, æ ¦ 30 C ,5 18~20\Î ßW Þ~-. ¯ \Ø ' 50 µl¾ ¤ % 50 µl BHI broth 10 ml , 7¼-µ. 37 C ,5 ßW Þ~-5 \ÎÙ ùú-. ! >öA spectrophotometer +O5 ìû6(600 nm) å@-µ. (7) pH å@ pH ®¯2 $? ¿ å@-µ. S. mutans !7 1 loop 10 ml BHI broth, æ ¦ 37 C,5 18~20\Î ßW Þ~-. ¯ \Ø ' 50 µl¾ ¤ % 50 µl BHI broth 10 ml , 7¼-µ. 37 C,5 ßW Þ~5 \ÎÙ ùú-. pH meter +O5 å@-µ. (8) ²P¯% @Å å@ Þt# ²P¯% ®¯2 phenol sulfuric acid aXA + -µ. ü' ºd2 §Å¬A yW b-. ü% D-glucose U+-µ9, glucose 100 µl/ml stock solutionA ýx, HP ôõ-. äÙ ü'A ýþ . §Å * 20 ml+ \·{, äÙ ü' 1 ml A

(17) -., .W, 80% phenol 25 µl ¾ ÿ= 2.5 ml A

(18) -x, º',2 ü' HP 1 ml A

(19) Ý. Q sample 1 ml? .W, 80% phenol 25 µl¾ ÿ= 2.5 ml A

(20) Ý. \·{A Ó ¦ 30 C,5 20KÎ ó\], ¶ ÌÀ $, ü' < º' ìû6 490 nm,5 å@Ý. (9) ° @Å å@ Þt# ° ®¯2 BCA kit +-µ. ü' ºd 2 §Å¬A yW b-. ü% BSA(Bovine Serum albumine)A +-µ. BSA 1 mg/ml stock solutionA ýx, HP ôõ-. äÙ ü'A ýþ. §Å * 20 ml + \·{, BCA Ó'(100:2)A 4 ml

(21) -., .W, äÙ ü'A ÀÀ 0.1 ml Ã

(22) -x, º ',2 ü' HP 0.2 ml A

(23) Ý. Q sample 1 ml , BCA reagent Ó'A

(24) Ý. \·{A Ó ¦ 37 C ,5 30KÎ ó\] ¶,5 ÌÀ $, ü' < º' ìû6 562 nm,5 å@Ý. (10) S. mutans GH K = ¤ % S. mutans glucan >, m 2 ©ªA «W bO Þ~' # GH ~ aX A ®R-. ¶-µ. \ ùú b-. BHI Þt , ¤ %A

(25) (2 mg/ml)-. À \ÎÙ Pf-. Þt 4 C Ì x, {-µ. {T Þt# GH M Å $? ¿ å@-µ. GH ÐW b-. ùúT Þt, 2.5N Õ6

(26) 16Ë NaOH

(27) -x 12\Î Ï a\í. aT Þ~', acetic acid

(28) -. 4¯ $ cell debris 8|K-. dB ¦, (', 3ÞÅ(v/ o. o. o. o. o.

(29) Standard extract preparation from herbal medicines.. A U+-µ. !7 < Þ~ ¶·, U+T !72 àáâ m>%á?,5 K~ ã S. mutans KCTC 3065 U+-µ9, { < äÞ~ brain heart infusion(BHI) Þt, 37 C,5 24\Î Ï ß WN,5 ßW Þ~-µ. (4) l! å@ À ¤ % l! §¨ paper disc method , -. å@-µ9 , S. mutans !7 1 loop 10 ml BHI broth, æ ¦ 37 C ,5 18~20\Î ßWN,5 ß W Þ~-. +-µ. ç!T Muller hinton agar Þt petri dish, 15 mlÃ K7-. xR¯ \Ø ¦, S. mutans Þ~' 50 µl æ. !s-S 6è-µ. é 63 ¤ %A 400 mg Ã ç!P 1 ml , êÔë . paper disc(6 mm), ÀÀ 50 µlÃ ìP ¦ º\í. ºT disc2 \·+ ÄîÞt , ï ð;\Ø $, 37 C ,5 24\Î Ï ßW Þ~-µ. %, l! paper disc 7® clear zone ñ¹(mm)A å@-. î-µ. (5) oJòOÕ6 å@ oJòOÕ6(MIC, minimum inhibitory concentration)2 paper disc method, -. å@-µ9 , S. mutans !7 1 loop 10 ml BHI broth, æ ¦ 37 C,5 18~20\ Î ßW Þ~-. +-µ. stirrer(Corning Co., USA) (3). o. 14) o. o. 14). o. o. o. 15). o.

(30) . Antimicrobial Activity of Streptococcus mutans by Herbal Medicine Extracts. Antimicrobial effects of a medicinal herb extracts. Continued. against S. mutans KCTC 3065 Serial No. DH-01 DH-02 DH-03 DH-04 DH-05 DH-06 DH-07 DH-08 DH-09 DH-10 DH-11 DH-12 DH-13 DH-14 DH-15 DH-16 DH-17 DH-18 DH-19 DH-20 DH-21 DH-22 DH-23 DH-24 DH-25 DH-26 DH-27 DH-28 DH-29 DH-30 DH-31 DH-32 DH-33 DH-34 DH-35 DH-36 DH-37 DH-38 DH-39 DH-40 DH-41 DH-42 DH-43 DH-44 DH-45 DH-46 DH-47 DH-48 DH-49 DH-50. clear zone Korean name Name of herbal medicines (mm) Sophorae flos Goihwa ND* Platycodi radix Gilkyung ND* Eucommiae cortex Duchung ND* Equiseti herba Soksae ND* Acanthopanacis cortex Ogapi ND* Achyranthis radix Usul 1 Persicae semen Doin ND* Kalopanacis cortex Haedongpi ND* Gastrodiae rhizoma Cheonma 0.6 Pogongyung Toraxaci herba ND* Moutan cortex radix Mokdanpi 0.5 Goldamcho Caraganae radix ND* Angelicae koreanae radix Ganghwal 1 Imperata rhizoma Mogun ND* Hwanggum Scutellariae radix 3.5 Salviae radix Dansam ND* Amomi cardamomi fructus Baekdugu 1 Rubi fructus Bokbunja 1 Chrysanthemi flos Gamguk 1.7 Meliae cortex Golyonpi ND* Torreyae semen Bija ND* Lycopi herba Jibuja 6.3 Baekdanhang Santali album lignum 0.5 Hydnocarpi semen Daepungja 0.5 Gumaengja `Rosae laevigatae fructus 1 Arecae semen Binlangja 0.8 Ganghwang Curcumae longae rhizoma 0.7 Cuscutae semen Tosaja ND* Ampelopsis radix Baeklyom ND* Dictamni radicis cortex Baeksunpi ND* Baekgulchae Chelidonii herba 0.5 Sophorae radix Gosam ND* Cibotii rhizoma Guchuk ND* Crataegi fructus Sansaja 1 Dioscoreae rhizoma Sanyak 1 Sophorae subprostratae radix ND* Sandugun Sukchangpo Acori graminei rhizoma 0.6 Choyongdam Labiatae flos ND* Phlomidis radix Sokdan 0.8 Cimicifugae rhizoma Sungma 0.7 Bupleuri radix Siho ND* Magnoliae flos Sini 1 amomi xanthioidis fructus Gongsain ND* Forsythiae fructus Yonkyo ND* Artemisiae asiaticae herba Aeyop 3 Katsumadai alpiniae semen Chodugu ND* Typhae pollen Pohwang ND* Lonicerae flos Indong 0.8 Anthrisci radix Jeonho 1 Zingiberis rhizoma Gungang 1.2. clear zone (mm) Epimedii Eumyangguk herba 3.3 Ramulus uncariae cum uncis Jogudong 1.5 Aconitum flos Jinbum ND* Sorbus cortex Magamok ND* Ephedrae herba Mahwang ND* Citrus unshin peel Jinpi 1 Rhei Rhizoma Daehwang ND* Zanthoxyli pericarpium Chuncho ND* Meliae fructus Chunlunja 1.2 Chungsangja Caricae fici fructus 1 Chukbaeyop Meliae flos ND* Benincasae semen Donggoaja ND* Danggui Angelicae gigantis radis ND*. Serial No. Korean name DH-51 DH-52 DH-53 DH-54 DH-55 DH-56 DH-57 DH-58 DH-59 DH-60 DH-61 DH-62 DH-63. Name of herbal medicines. *ND: Not Detective. v) ,²A

(31) -. -v Ï a\í. ,², O ÔT GH2 xu8|KW(Beckman XL-90) Ô\] (15,000 g, 10 min) GH KA K-µ. KT G H2 60 C,5 º\Ø ¦ 4Å(mg/ml)A å@-µ. o.

(32). . ¤: %A Ðw S. mutans, l !I?¾ A paper disc method å@ ø?2 Table 2¾ ¿. S. mutans , O5 z , h, °ª, N,

(33) YN, ÿ, ° ù, õE, u, 1 mm - òO. ñ¹A e&#þ9, , , °, KN, N, = UN, =, , Ô), h, (N 1 mm ! cdA µx, 1.7 mm, 1.2 mm, º 1.5 mm, zN

(34) 1.2 mm ! cdA µ. Q $~ 3.3 mm, 3 mm ! cdA µ9, ë t:N, ÿ 2 ¤ %,5 òO. ñ¹A e&#w l! 1þ9(Figs. 1, 2), t:N 2 6.3 mm, ÿ 3.5 mm e&(Table 2). 2.

(35) S. mutans , O Lâ } l!A e& ¤ % t:N, ÿA ( oJòOÕ6 å@ ø? 2 Table 3? ¿. t:N % ¹2 2.5 mg/ml

(36) \ >ö {÷1t 9, ÿ % \ 2.5 mg/ml

(37) \ >ö {÷1 t . ^_5 S. mutans , t:N, ÿ % oJòOÕ62 5 mg/ml e&(Figs. 3, 4). 3. S. mutans ¤ % S. mutans >ö, m2 I? å@ -W bO t:N, ÿ %A 2 mg/ml Õ6

(38) 1.. 63.

(39) . Journal of Dental Hygiene Science Vol. 2, No. 1, pp. 0~0 (2002). Antimicrobial activity by paper disc method of the. Minimal inhibitory concentrations of the Scutellariae. DH-15 Scutellariae radix extracts on the growth of S. mutans KCTC 3065.. radix extracts on the growth of S. mutans KCTC 3065. (1) 10 mg/ml (2) 5 mg/ml (3) 2.5 mg/ml (4) 1.25 mg/ml. Antimicrobial activity by paper disc method of the DH-. Minimal inhibitory concentrations of the Lycopi herba. 22 Lycopi herba extracts on the growth of S. mutans KCTC 3065.. extracts on the growth of S. mutans KCTC 3065. (1) 10 mg/ml (2) 5 mg/ml (3) 2.5 mg/ml (4) 1.25 mg/ml. .. Minimal inhibitory concentrations of the herbal medicines extracts on the growth of S. mutans KCTC 3065 The herbal medicines extracts. Minimal growth inhibitory concentrations mg/ml of the medicinal herb extracts. Scutellariae radix Lycopi herba. 5 5. ¦ ! >öA spectrophotometer å@ ø? Figs. 5-8 ? ¿. (1) > ¬, m2 ©ª t:N % S. mutans > , I?2 º! Þ~ 8\Î ¦, ìû6 1.05 o > A µe, t :N %(2 mg/ml)A ". Þt,52 > tr1w 16\Î ¦, ìû6 0.91 o > A e&#. ÿ % ¹ º! Þ~ 8\Î ¦, 1.05 o > A µ9, ÿ%(2 mg/ml)A ". Þt,52 > t r1w 16\Î ¦, 0.87 o > A e&#(Fig. 5). ¾ ¿ ø?2 dT \ÎÏ #, ÑH-. l I? e&#2 , $d K ¤ %A

(40) - A I? àj P kA i UT.. Growth curve of S. mutans by addition of Lycopi herba and Scutellariae radix extracts. - - Lycopi herba, - - Scutellariae radix, - - Control. . . . ®¯, m2 ©ª t:N %A S. mutans Þt,

(41) -. 8\Î Ï Þ~ ¦ pH å@ ø? º! pH 5.08 %& ® ¯ µtý, t:N % ".T Þt2 pH 5.80 Lâ êý ®¯ µ. ÿ % ¹ º! pH 5.08 %& ®¯ µtý, pH 5.74 Lâ êý -S J-µ(Fig. 6). (2) pH.

(42) . Antimicrobial Activity of Streptococcus mutans by Herbal Medicine Extracts. The pH changes in S. mutans culture medium by addition of Lycopi herba and Scutellariae radix extracts - - Lycopi herba, - - Scutellariae radix, - - Control . . . . (? ¿ S. mutans Þt# pH ®¯, 0T ¤ % I? « ø? S. mutans ! ¬? ðæ ({{ä

(43) k$A « P kþ. pH2 @( ¹2 4tý, GA ' (ú -2 ¹,2 !"# !, O = >1w pH

(44) )wtx, 5.4 - )wtS 1 A /0. ^_5 ¶· ø? t:N, ÿ % * º!, LO pH %& ®¯ ! cd¾ ¿ aX cd-2 i e&+ ª¦ l I? Wj P kA i UT. (3) ²P¯% ®¯, m2 ©ª = ¤ %A S. mutans,

(45) ¦ \ÎÙ Þ ~\ Þt# ²P¯% ®¯ «. º!e t: N %

(46) T Þt qW ± ²P¯% ~ mlG 2.5~3 mg ²P¯% @Å1þ. Þ~ 8\Î ¦ º! 0.81 mg/ml þe, t:N % ".T Þt2 1.70 mg/ml ²P¯% ~ B ® -t 9, ÿ % ¹ \ Þ~ 8\Î, 1.84 mg/ml ²P¯% ~ B ®-t (Fig. 7). (4) ° ®¯, m2 ©ª ¤ %

(47) T Þt, S. mutans KCTC 3065 Þ~-x \ÎÙ ®¯-2 ° ÅA å@-µ. Þ~ 0\Î ,52 *, Þt,5 10 mg/ml ° @Å1þ. ! o > .A 8\Î,52 º! 8.39 mg/ml þx, t:N % ".T Þt2 9.52 mg/ml ° @Å1þ(Fig. 8). t:N2 ´7?, u-2 s/> q 0¾ O1*+ k. K, { r Nazar Kochia indica,5 monobromo derivative < Pt alkaloid K reineckate ø@A K-µx, Tandon Kochia trichophylla benzene < ,² K,5 saturated hydrocarbon hentriacontane, hentriacontanol, triterpenoid ä¾ saponin oleanolic acid W -. glucose, arabinose < rhamnose

(48) øÓ1w k$A x-µ9, Kernan t:N N +-. oleanolic acid @ÅA x ½

(49) k. S. mutans, t:N r2 Ô2-. 3 6 ' r

(50) 4nj i UT. ÿ % ".T Þt2 9.28 mg/ml ° @Å 16). 17). 18). 19). The changes of total carbohydrate in S. mutans culture medium by addition of Lycopi herba and Scutellariae radix extracts. - - Lycopi herba, - - Scutellariae radix, - - Control. . . . Protein changes in S. mutans culture medium by addition of Lycopi herba and Scutellariae radix extracts. - - Lycopi herba, - - Scutellariae radix, - - Control . . . . 1þ(Fig. 8). ÿ l5~, l < l!*+ @1w 6 U+1w7 8. 7K2 wogonin, baicalein, baicalin t9t 40. flavone < {¯Ó% K x 1þ9 , y *+2 :;Þ<=*+ , l«>?W *+ , t U*+ «@7 k. F,6 ÿ %# baikalin flavonoid

(51) >-# )?A l(, ©ªA 7x l < l¹ I?6 k2 x

(52) k . ^_5 3 , ÿ *+, {-. ' r

(53) vw7B j i UT. 20). 22). 21). 23). 24). S. mutans! "#$ % &' S. mutans GH2 , {.-2 !"A R-2C

(54) 4n jA -2 GH. ^_5 r,52 ¤ % S. mutans GH >A w D @6 cd-2t -W b-. '-Þt,5 \ÎÙ GH ~ ®¯ «. Table 4,5 2 ½¾ ¿ º!,52 Þ~ 8\Îý, 300 mg/100 ml GH Ð þe, t:N ¹ 200 mg/100 ml, ÿ 220 mg/100 ml GH

(55) >1w, º! L 50% JI? µ 4.. (Fig. 9)..

(56) Journal of Dental Hygiene Science Vol. 2, No. 1, pp. 0~0 (2002). Effects of herbal medicines on S. mutans KCTC 3065 polysaccharide production Time (hr) polysaccharide (mg/100ml cultured medium) Sample Names Scutellariae radix Lycopi herba Control. 0. 8. 12. 16. 24. 48. 16 15 20. 220 200 300. 200 180 280. 175 170 220. 155 165 220. 150 160 210. . 8\Î Ï Þ~ ¦ Þt# ²P¯% ® ¯ÅA å@ ø? º! 0.81 mg/ml þ9, t: N, ÿ % ".T Þt2 1.70 mg/ml, 1.84 mg/ ml þ. 5. S. mutans 8\Î Ï Þ~ ¦ Þt# ° ®¯ ÅA å@ ø? º! 8.39 mg/ml þ9, t:N, ÿ % ".T Þt2 9.52 mg/ml, 9.28 mg/ml þ. 6. S. mutans GH >, m2 I?2 º! ¹ Þ~ 8\Î, 300 mg/100 ml A >-µe, t: N, ÿ % ".T Þt,52 200 mg/100 ml, 220 mg/100 ml A >-µ. 4. S. mutans. . The effect of Lycopi herba and Scutellariae radix on S. mutans KCTC 3065 polysaccharide production. - - Lycopi herba, - - Scutellariae radix, - - Control. . . . 2 qW ".Å oJcdÕ6 ml G 2 mg ".ÝW EF i U19, r GH Kf ¯á A ½ glucan GHµ. ( ø? t:N, ÿ % !" R, 4 n-S *+-2 S. mutans GH >A cdj i .Gt9 % + l % n, Áë ój P k2 zr J¤3 m

(57) k2 i UT. 25). . r,52 #,5 /V12 63 ¤ ¥P % S. mutans, l!I? paper disc method § ¨-., l!I?

(58) P t:N, ÿ S. mutans > , { ©ªA Ä

(59) -µ. 1. 63 = ¤ ¥P % ª!I? H¨ ø? S. mutansl, ª! P I52 t:N, ÿIþ. Paper disc method, l! å@ (20 mg/ml ".) ø?2 t:N

(60) 6.3 mm, ÿ 3.5 mm þ. 2. ¤ % S. mutans > , I?2 º! 8\Î,5 1.05 o > .A µe, t:N, ÿ % 2 mg/mlA ". Þt,52 16\Î ý, 0.91, 0.87 o > A µ. 3. S. mutans 8\Î Ï Þ~ ¦ Þt# pH å@ ø? º! 5.08 þ9, t:N, ÿ % ".T Þt2 5.80, 5.74 þ.. 1. Hamada S, Koga T, Ooshima T: Virulence factors of streptoccus mutans and dental caries prevention. J Dent Res 63: 407-411, 1984. 2. Gibbons RJ, van Houte J: Bacterial adherence in oral microbial ecology. Annu Rev Microbiol 29: 19-44, 1975. 3. McGhee JR, Michalek SM: Immunobiology of dental caries Microbial aspects and local immunity. Ann Rev Microbial 35: 595-635, 1981. 4. Namba T, Tsunezuka M, Hattor M, Kadota S, Kikuchi T: Studies on dental caries prevention by traditional chinese medicines Screening of crude drugs for inhibitory action on plaque formation. Proc. Symp. WAKAN-YUKU 15: 179-186, 1982. 5. Miyoshi M, Imoto T, Kasagi T: Antieurodontic effect of various fraction extracted from the leaves of Gymnema sylveser. J Yonago Med Ass 38: 127-137, 1987. 6. Nakahara K, Kawabata S, Ono H, Ogura K, Tanaka T, Ooshima T, Kamada S: Inhibitory effect of Oolong tea polyphenols on glycosyltransferase of mutans streptococci. Appl Environ Microbiol 59: 968-973, 1993. 7. Ooshima T, Minami T, Aono W, Izumitani A, Sobue S, Fujiwara T, Kawabata S, Hamads S: Oolong tea polyphenols inhibit experimental dental caries in SPF rats infected with mutans streptococci. Caries Res 27(2): 124-129, 1993. 8. Otaka S, Makimura M, Kuroki T, Nishihara Y, Hirasawa M: Anticaries effects of polyphenolic compounds from Japanese green tea. Caries Res 25(6): 438-443, 1991. 9. Sakanaka S, Kim M, Taniguchi M, Yamamoto T: Antibacterial substances in Japanese green tea extract against Streptoccus mutans-A catiogenic bacteruium. Agr Bio Chem 53(9): 23072311, 1989. 10. Takasago K: Inhibitor of cariogenic bacteria. Japan patent 25890, 1990. 11. , , , , , , : , Streptococcus mutans hydroxyapatite . 20(2): 259-270, 1996. 12. , , : . 40(4): 478-481, 1996. 13. , , , , , , , : , Streptococcus mutans . 20(1): 107-120, 1996. 14. Marvin T, Robert I, Lindemeyer, Robert G. Petersdorf: Comparison of single-disc and tube-dilution techniques in determining antibiotic sensitivities of gram-negative pathogens. Annals of Internal Medicine 58(1): 56-65, 1962. 15. Han MD, Jeong H, Lee JW, Back SJ, Kim SW, Yoon KH: The composition and bioactivities of ganoderan by mycelial fractionation.

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(63) . 16. 17. 18. 19.. 20. 21.. 22.. Antimicrobial Activity of Streptococcus mutans by Herbal Medicine Extracts of Ganoderma lucidum IY009. Kor J mycol 23(4): 285-297, 1995. : . . 361, 1984. Nazar Singh, I.S. Nirula: Chemical investig ation of Kochia indica. Indian. J Pharm 21(4): 4-7, 1958. Tandon JS, Agarwal NP: Chemical examination of Kochia trichophylla, Indian. J Chem 4: 545, 1966. Jack A, Kernan, EwenCoxworth, Sharon Fleming: Microdetermination of triterpene sapogenin content of Kochia scoparia seed using Gas-liquid chromatography. J Agr Food Chem 21(2): 232-234, 1973. Tang W, Eisenbrand G: Chinese Drugs of plant origin. Springer Verlag pp. 919-929, 1992. Kenichi Abe, Osamu Inoue, Eizaburo Yumioka: Biliary excretion of metabolites of baicalun and baicalein in rats. Chem Pharm Bull 38(1): 208-211, 1990. Kubo M, Matusuda H, Kimura Y, Okuda H, Higashino M, Tani. QRS TUVWXYZ 21[\]. T, Namba K, Arichi S: Studies on Scutellariae Radix. VII. Antiarthritic and anti-inflammatory action of methanolic extract and flavonold components from Scutellariae Radix. Chem Pharm Bull 33: 2411-2415, 1984. 23. Kubo M, Matusuda H, Kimura Y, Okuda H, Arichi S: Scutellariae Radix. X. Inhibitory effects of various flavonoids on histamine relelease peritonial mast cells in vitro. Chem Pharm Bull 32: 2724-2729, 1984. 24. Kimura Y, Okuda H, Tani T, Arichi S: Studies on Scutellariae Radix. IV. Effects of various flavone compounds on lipid peroxidation in rat liver. Chem Pharm Bull 30: 1792-1795, 1982. 25. , , , , : Streptococcus mutans KCTC 3065 . 24(3): 259-270, 2000. (Received May 28, 2002; Accepted June 24, 2002). ^_ ` ab c dc >efg >? hOi \.j k< * + ,-./0.

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