Effect of the Extract of Schizandra chinensis Baill on Bacteria Isolated from Oral Cavity

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(1)Journal of Dental Hygiene Science Vol. 2, No. 2, pp. 85~ 88 (2002).

(2) (Schizandra chinensis Baill) . †. .

(3) . . 1. Effect of the Extract of Schizandra chinensis Baill on Bacteria Isolated from Oral Cavity †. Hyun-Ja Jeong , Yeong-Ae Lee and Won-Dae Ji1 Dept. of Dental Hygiene, Daegu Health College. Daegu 702-722, Korea Dept. of Food Science and Technology, Yeungnam University, Kyongsan 712-749, Korea. 1. Growth inhibition of the extract of Schizandra chinensis Baill on bacteria isolated from oral cavity of dental patient was investigated. The extract yield of water was 53.8%, and the yield was higher than these of methanol. High temperature was profitable to extract yield. As a result of investigation about antibacterial effect of water extract of S. chinensis, tested strains were mostly inhibited by water extract of S. chinensis. As a result of investigation about minimal inhibitory concentration(MIC) of water extract of S. chinensis on the oral bacteria, MIC of water extract of S. chinensis was between 125 and 1000 µg/ml.

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(12) . Effect of the Extract of Schizandra chinensis Baill on Bacteria Isolated from Oral Cavity. W ÑL`$ Ãu cSÌÍ v}Ò$ - cSÌ". CHJ-4 , CHJ-6 , pascens CHJ-12 CHJ-7 , CHJ-8 , CHJ-9 , CHJ-10 , Brain Heart Infusion(Difco, U.S.A: BHI) .. . 2.. ¾m w£ OÆu Ó Ô Õ¨

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(17). ~zm pÝ9 OÆ| ûL~ ¬0 w üý] S@õ W OÆÅºõ OÆå Ç_Ì". w üý] OÆS@ cS0 OÆå |c9 ¶ Fig. 1 þ". w OÆS@ cS¯ ¢ OÆåu. . Yield of methanol extract from Schizandra chinensis Baill at different temperature. S. chinensis was extracted with methanol for 5hrs.. _ ÿò, mS@_ üý] OÆw 23.4% ) " 9t, ¹Å 80 C OÆå |c9 ¶ Fig. U Èg þ ¹Å OÆå 24.8% _ ÿò, OÆåu 25.5% | ¥ )". %& OÆå ¥~ ¬M w S@ cS0 OÆÅº- ¥ J U" H¯ J c«¦".. 53.8% . 2 80oC. 2.. o. . lZ v} 59 wOÆw ¾m |c9 ¶ Table 2g þ". A. pascens CHJ-12 59 M Ó 16 mm ;Ê Ú )D, "ó Ñ L_ CHJ-4, A. pascens CHJ-2 , ÑL_ CHJ-6 ¨¨ 15 mm, 13 mm, 12 mm M Ú~)s". ÑL_ CHJ-7 CHJ-9 W CHJ-10 50 11 mm M )sD, B. japonicum CHJ1 50 10 mm M )sÍ, S. epidermidis CHJ-3 50 ;Ê Ru 9 mm M )s". 9t, ÑL_ CHJ-8 59 Mu

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(19) . Growth inhibition of water extract(2 mg/20 µl) of Schizandra chinensis Baill on bacteria isolated from oral cavity of dental patient Strains. B. japonicum CHJ-1. 10. A. pascens CHJ-2. 13. S. epidermidis CHJ-3. a. . Yield of solvent extracts from Schizandra chinensis Baill. S. chinensis was extracted at 80oC for 5hrs.. Inhibition zone diameter (mm). 9. Unidentified strain CHJ-4. 15. Unidentified strain CHJ-6. 12. Unidentified strain CHJ-7. 11. Unidentified strain CHJ-8. −a. Unidentified strain CHJ-9. 11. Unidentified strain CHJ-10. 11. A. pascens CHJ-12. 16. No inhibition. Diameter of paper disc was 7 mm. Cells were cultivated at 37oC for 48 hours in BHI plate..

(20) . Journal of Dental Hygiene Science Vol. 2, No. 2, pp. 85~88 (2002)

(21) . Minimal inhibitory concentration of water extract of Schizandra chinensis Baill on bacteria isolated from oral cavity of dental patient Strains. Concentrations(µg/ml) 2000. 1000. 500. 250. 125. 6.25. 3.125. MIC (µg/ml). B. japonicum CHJ-1. -. -. +. ++. ++. ++. ++. 1000. A. pascens CHJ-2. -. -. -. -. +. +. ++. 250. S. epidermidis CHJ-3. -. -. +. +. ++. ++. ++. 1000. Unidentified strain CHJ-4. -. -. -. -. -. +. ++. 125. Unidentified strain CHJ-6. -. -. +. +. ++. ++. ++. 1000. Unidentified strain CHJ-7. -. -. +. ++. ++. ++. ++. 1000. Unidentified strain CHJ-9. -. -. +. ++. ++. ++. ++. 1000. Unidentified strain CHJ-10. -. -. +. ++. ++. ++. ++. 1000. A. pascens CHJ-12. -. -. -. -. -. +. ++. 125. Symbols: ++; growth, +; weak growth, -; no growth Strains were cultured at 37oC for 48hrs in BHI plates added with each concentration of water extract of Schizandra chinensis Baill.. lZÁ 59 §ôMº- |cÌÍ, E ¶ Table 3 þ". wOÆwu ÑL_ CHJ-4 A. pascens CHJ-12 5M 125 µg/ml ;Ê u §ô Mº- )sD, ÑL_ CHJ-2 g CHJ-11 5M 250 µg/ml §ôMº- )sÍ, ) $ é 5M 1000 µg/ml §ôMº- )s". wOÆw ÑL_ CHJ-4 A. pascens CHJ-12 50 ;Ê Z9 ¾ m ;!" J J c«¦". 9 ¶ $ lZ 79 Bacillus 59 OÆw MIC

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(27) , pp. 336-337, 1965. : . , pp. 268-269, 1988. : . !"

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(29) 89 , 1989. 5. %:;, <=>, ?@A, B: 1C', D, %&', %EF G HI J, JK&LM, CM N OPI ,=. /QR( ST, 22(1): 76-81, 1990. 6. UVW, /$X, Y#&, Z[, ,=\, ]=, ^, <^_, `>!: %&'(Schizandra chinensis Baili.) abcde fgh i =jI J. /Qkl ST, 43(1): 72-77, 2000. 7. m#=: %&' GHn oTpq rs- &tu !v. /Q!w x ST, 24(5): 658-662, 1995. 8. `y[: 1z& . &( M{, 16(3): 32-35, 1990. 9. ,|}: 8:~&g(Phase contrast microscope) nA/ 1z &

(30) |A. t(01, 21(3): 37-44, 1987. 10. <!, 0, % : Alternaria alternata- fn u 0 ,

(31) . /Q@J ST, 12(1): 66-71, 1996. 11. <0, n!', C, :, n*0, <: 0cde 0 - ./ 01. RIRY]d0, 1: 75-93, 1997. 12. ': 0'6 nA/ 1z +B(- ./ 01. 51 5 ( 5 6 7

(32) 89 , 2001. 13. l, /T2, < 2: C N I -O GHn Listeria monocytogenesI q +- &tu !v. /QR( ST, 26(5): 627-632, 1994. 14. ¡¢ £: ¤¥¦§¨©ª« - ¤¥¬¨®¯°±²³ -. ´µ¶·¸ ¹º»¼½¼¾, ¿À, 1979. 1. 2. 3. 4..

(33) . Effect of the Extract of Schizandra chinensis Baill on Bacteria Isolated from Oral Cavity. T65, Á>, Â*, <=!, Ug0, !: 1z- 5/ Ã /+I q+. /Q8( ST, 3(1-2): 21-30, 1997. 16. <Tl, Äg, Å>: 1zI Æ- 5/ Ã vI ! v. /Q8( ST, 4(2): 81-86, 1988. 17. <3>, n2, <!y: 1z- 5/ ÇI fB(. /Q8 ( ST, 5(2): 111-119, 1989. 15.. (Received November 4, 2002; Accepted December 9, 2002). Table 1. ®$%"..

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