Effects of Glycyrrhizae Radix Pharmacopuncture Intravenous Injection on Ischemia-Induced Acute Renal Failure in Rabbits

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(1)ISSN 2093-6966. Journal of Pharmacopuncture. 5 http://dx.doi.org/10.3831/KPI.2011.14.4.005. Original Article. 허혈로 유발된 토끼의 급성 신부전에 대한 甘草 약침액 정맥 주입의 영향. Received : Sep 4, 2011 Revised : Oct 17, 2011. Effects of Glycyrrhizae Radix Pharmacopuncture Intravenous Injection on Ischemia-Induced Acute Renal Failure in Rabbits. Accepted : Nov 2, 2011 KEY WORDS: Glycyrrhizae Radix; pharmacopuncture; ischemia; renal failure. Hyeong Cheol Kim1, Gyoung Ho Kim2, Guem San Lee2, Hyung Woo Kim2, Se Hyun Lim3, Chi Yeon Lim4, Young Gyun Kim1, Su In Cho2* 1 Dong-Eui University College of Oriental Medicine, Busan, Korea Pusan National University School of Korean Medicine, Yangsan, Korea 3 Department of Nursing, Far East University College of Public Health, Eumseong, Korea 4 Department of Medicine, Dongguk University College of Medicine, Seoul, Korea 2. Abstract Objectives: The present study was undergone to determine whether Glycyrrhizae Radix pharmacopuncture intravenous injection exerts beneficial effect against the ischemia-induced acute renal failure in rabbits. Methods: Rabbits were treated with Glycyrrhizae Radix pharmacopuncture via i.v., followed by renal ischemia/reperfusion. The fractional excretion of glucose and phosphate were measured and the malondialdehyde content was also determined. The morphological changes of cortical part of kidney also observed with light microscope. Results: Renal ischemia/reperfusion caused increase of the fractional excretion of glucose and phosphate in ischemia-induced animals, which was prevented by Radix Glycyrrhizae extract treatment. Ischemia/reperfusion increased lipid peroxidation, which was prevented and morphological changes also altered by Radix Glycyrrhizae pharmacopuncture administration. Conclusions: These results indicate that lipid peroxidation plays a critical role in ischemia-induced acute renal failure and Glycyrrhizae Radix pharmacopuncture exerts the protective effect against acute renal failure induced by renal ischemia/reperfusion.. I. 서론. cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.. 2011 Korean Pharmacopuncture institute. Corresponding Author Su In Cho, Division of Pharmacology, School of Korean Medicine, Pusan National University, Yangsan, Korea Tel: +82-51-510-8457, Fax: +82-51-510-8420 E-mail: [email protected] http://pharmacopuncture.co.kr.

(2) 6. Journal of Pharmacopuncture 제 14권 제 4호( 2011년 12월). II. 재료 및 방법 1 1.1 2.0. New Zealand White 2. 60. 1.2 72. 2 2.1. nosae bra L.. tyrosinase. LegumiG. glaGlycyrrhiza uralensis Fisch (G. inflata Bat.). 1,000 80g 30 DWP-99000T, 2 500 5.9g 1% 800 g 15 Whattman paper No 1 0.45 membrane filter (Scheme 1). pH 0.25. 7.2. pore size. Scheme 1. Procedure of preparing Glycyrrhizae Radix pharmacopuncture i.v. solution..

(3) 7. 2.2. 1% phosphoric acid 3 0.6% thiobarbituric acid 1 60 1-Butanol 4 800 g 25 534nm 510nm MDA 1 pmoles Bradford. sham operation ketamin. xylazine. 15. /. 2.5. / 5. 1. /. /. 30 2.6 silver clip. 1. 4% paraformaldehyde paraffin. 1. 24 microtome slide glass. vehicle vehicle vehicle 2.3 1 0.2. Periodic acid-Schiff (PAS) hematoxylin Canada balsam (Olympus BX51, Japan). /min/ 1 3. 4 72. 2.4 glucose. phos-. Sigma plot (Sigma plot for Windows, ver. 9.0, U.S.A.) (mean SD) Student's ttest p0.05. phate (Pi). III. 결과 2.5. malondialdehyde (MDA). 130mM NaCl, 5mM KCl, 10mM Tris-HCl (pH 7.4). (Tomas Co. USA) Uchiyama MDA. Mihara. Stadie-Riggs microtome 1 0.3 0.5. 1. Glucose. 0.24 4.92. 0.31% Pi 120.3. 0.51% 27.0. 12.2%. 0.05% 2.63 (Fig. 1).. 4.8% 92.4 8.6% (Fig. 2).

(4) Journal of Pharmacopuncture 제 14권 제 4호( 2011년 12월). 8. Fig. 1. Effect of Glycyrrhizae Radix pharmacopuncture treatment on changes in fractional glucose excretion (FE glucose) in ischemia/reperfusion induced acute renal failure. Data are mean S.E. of five experiments. NOR, normal group; CON, 1 hr of renal ischemia applied group; SAM, 1 hr of renal ischemia and 1% concentration of Glycyrrhizae Radix extract treated group. *, p 0.05.. Fig. 3. Effect of Glycyrrhizae Radix pharmacopuncture treatment on changes in lipid peroxidation of cortex of kidneys in ischemia/reperfusion induced acute renal failure. Data are mean S.E. of five experiments. NOR, normal group; CON, 1 hr of renal ischemia applied group; SAM, 1 hr of renal ischemia and 1% concentration of Glycyrrhizae Radix extract treated group. *, p 0.05.. 3.. PAS (brush border) (Fig. 4A) (parietal layer) (visceral layer) (capsular lumen) Fig. 2. Effect of Glycyrrhizae Radix pharmacopuncture treatment on changes in fractional Pi excretion (FE Pi) in ischemia/reperfusion induced acute renal failure. Data are mean S.E. of five experiments. NOR, normal group; CON, 1 hr of renal ischemia applied group; SAM, 1 hr of renal ischemia and 1% concentration of Glycyrrhizae Radix extract treated group. *, p 0.05.. 2.. protein protein MDA/ protein (Fig. 3).. PAS. PAS (Fig. 4-B). PAS. 90.2 5.3 pmole MDA/ 138.0 8.9 pmole MDA/ 110.4 5.8 pmole. (Fig. 4-C).

(5) 9. A. B. 150g. 4. 200. C Fig. 4. Light micrograph of kidney from normal(A), control(B), and sample(C) groups. G, glomerulus; PT, Proximal tubule; DT, distal tubule. PAS-stain 200.. IV. 고찰. 1% 2 12g 15 30g glucose. Pi.

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