서론
1960 Mester
(biostimulation effect)
. Jan Tunér1) ,
(systemic effect) ,
.
(therapeutic laser)
InGaAlP 레이저 경피혈액조사가 정상성인의 심박변이도에 미치는 영향에 대한 임상적 연구(Ⅱ)
여진주, 이태호, 손동혁, 형례창, 이인환*, 장인수
우석대학교 한의과대학 내과학교실, 전북대학교 의과대학 신소재공학부*
A Clinical Study of InGaAlP Laser Transcutaneous Blood Irradiation on Heart Rate Variability in Healthy Adults (Ⅱ)
Yeo Jinju, Lee Taeho, Son Donghyuk, Hsing Lichang, Lee Inhwan*, Jang Insoo
Department of Internal Medicine, College of Korean Medicine, Woosuk University Faculty of Materials Science and Engineering, Chonbuk National University*
Objective Objective Objective
Objective :::: The heart rate variability is very useful indicator to study the function of the autonomic nervous system(ANS), and the physiologic signals can be observed based on the changes of the ANS of the heart. In order to assay the effects of the laser exposing to healthy subjects, the double blind test has been performed.
Methods Methods Methods
Methods :::: This study included 62 healthy adults who have not any ANS disease and had normal sinus rhythm in electrocardiogram. The control group consisted of 31 subjects, laser group consisted of 31 subject. HRV was measured for 5 minutes before laser irradiation, sham and real laser irradiated for 30 minutes and than HRV remeasured for 5 minutes. Statistical significance was evaluated by independent T-test.
Results Results Results
Results :::: Mean HRV, Ln(VLF), Ln(HF), Ln(TP) of both groups at post-laser period decreased compared with that of the pre-laser period. Ln(LF) of both groups at post-laser period increased compared with that of the pre-laser period. LF/HF, SDNN of real laser group decreased and sham group decreased.
Conclusions Conclusions Conclusions
Conclusions :::: There is no difference between two groups. The reason is presumed that all the studied subjects are healthy adults, and also the short and single transcutaneous laser irradiation would not influence upon changes of the ANS. The further study must be followed.
Key Key Key
Key Words:Words:Words:Words: Heart rate variability(HRV), Power spectrum analysis(PSA), Laser therapy, Therapeutic laser, InGaAlP Laser, Autonomic nervous system
(low level laser) , (surgical laser) .
(laser acupuncture)
.
HeNe (633 nm) ,
(630-685 nm) Indium-Gallium-Aluminium-Phosphide
(InGaAlP) 1).
,
.
(Heart rate variability:
HRV) ,
2),3)
.
HRV InGaAlP
4) .
.
연구대상 및 방법
1. 연구대상
2005 1 2005 5
,
(sinus rhythm) 62 (
38 , 24 ) .
2 ,
(Table 1).
2. 시험방법
20-25 . 5
.
5 ,
30 , 5
40 .
HRV CANS 3000(Central
& Autonomic Nervous System. Laxtha Inc. Products.
Korea)
(electrode) 5
.
. 5 HRV
(time domain analysis) (heart
rate), SDNN(standard deviation of all normal R-R
intervals) , (frequency domain
analysis) (total power, TP), (low frequency power, LF), (high frequency power, HF) ,
[inclusion criteria]
20 40
(sinus rhythm)
[exclusion criteria]
,
HRV 2 ,
(log-transformed low frequency power,
Ln(TP)), (log-transformed high
frequency power, Ln(HF)),
(log-transformed low frequency power, Ln(LF)),
LF/HF Ratio .
630 nm InGaAlP Lapex-2000(Meridian Co. Korea)
. (Cephalic
vein, Basilic vein) probe
. 40 mW
continuous mode 30 .
Sham Light Emitting
Diode(LED) , Lapex-2000
( ) probe
. LED
GaAlAs raw material ,
660±20 nm , Emitted Color .
2.00-2.04 mW/cm2 .
sham real InGaAlP
, .
, probe
power
, .
, real
( ) sham ( ) .
3
sham real probe
,
. HRV
(Figure 1).
Technical parameters Real laser probe Sham laser probe
1 2 3 4 5 6 7 8 9 10
, ,
,
Lapex-2000, ( ) , 630 nm, InGaAlP
, (collimated) 1
probe
40 mW
203.82 mW/cm2 (probe )
Class 3B
, ( )
660±20 nm, GaAlAs LED ,
2.00-2.04 mW
10.19-10.39 mW/cm2(probe )
3. 레이저 파라미터(Laser parameter)1)
, ,
Table 2, 3, 4 .
4. 통계 처리
SPSS for window 10.0 , HRV
independent t-test , p-value 0.05
.
결과
1. 일반적 특성
27.27±4.34(19-37) (n=31) 27.77±4.42(19-37 ), (n=31) 26.77±4.26(20-37
) .
2. 레이저 시술 전 HRV
5 5 HRV
.
3. 레이저 시술 후 HRV
Mean HRV 73.39±8.34 cycle/
min 30 73.26±9.12 cycle/min
. SDNN 44.26±15.55
ms 30 42.71±12.10 ms
. Ln(VLF) 6.54±0.85 logms2
30 6.43±0.71 logms2
. Ln(LF) 6.18±0.86 logms2
30 6.21±0.79 logms2
. Ln(HF) 5.66±0.86 logms2
30 5.52±0.96 logms2 .
Treatment parameters Real laser probe Sham laser probe
1 -Cephalic vein, Basilic vein
-0.20 cm2 , point -1
2 : 366.88 J/cm² 18.342-18.702 J/cm2
3 366.88 J/cm² 18.342-18.702 J/cm2
4 : 203.82 mW/cm2 10.19-10.39 mW/cm2
5 4
6 ,
7 , spot 0.5 cm
8
9 , ,
10 1
11 1
Medical parameters Real laser probe Sham laser probe
1 2 3 4 5 6 7 8
(Dropout rate)
( , )
31 , 27.77±4.42(19-37) (M 21, F 10) Table 1
0%
31 , 26.77±4.26(20-37) (M 17, F 14)
Ln(TP) 7.38±0.74 logms2 30 7.33±0.61 logms2
. LF/HF ratio 1.37±1.52
30 1.31±1.54 .
Mean HRV 75.58±10.53 cycle/
min 30 74.48±9.91 cycle/min
. SDNN 42.45±12.36
ms 30 43.35±12.15 ms
. Ln(VLF) 6.31±0.75 logms2
30 6.25±0.79 logms2
. Ln(LF) 6.09±0.67 logms2
30 6.18±0.62 logms2
. Ln(HF) 5.95±0.85 logms2
30 5.86±0.72 logms2 .
Ln(TP) 7.28±0.62 logms2
30 7.27±0.58 logms2 .
LF/HF ratio 1.15±0.21
30 2.70±0.12 ,
.
30 Mean HRV, SDNN, Ln (VLF), Ln(LF), Ln(HF), TP, LF/HF ratio
Table 5 , independent t-test
Table 5 .
고찰 및 결론
(Laser) Light amplification by stimulated emission of radiation
. ,
, .
, ,
, .
, ,
,
group(probe) Pre-laser period Post-laser period Δ P-value
Mean HRV Laser
Sham
73.39±8.34 75.58±10.53
73.26±9.12 74.48±9.91
-0.06±3.75
1.10±2.83 0.506
SDNN Laser
Sham
44.26±15.55 42.45±12.36
42.71±12.10 43.35±12.15
1.55±9.52
-0.90±8.64 0.782
Ln(VLF) Laser
Sham
6.54±0.85 6.31±0.75
6.43±0.71 6.25±0.79
0.11±0.70
0.06±0.69 0.583
Ln(LF) Laser
Sham
6.18±0.86 8.03±10.96
6.21±0.79 6.18±0.62
-0.02±0.59
-0.09±0.47 0.528
Ln(HF) Laser
Sham
5.66±0.86 5.95±0.85
5.52±0.96 5.86±0.72
0.14±0.50
0.09±0.55 0.918
TP Laser
Sham
7.38±0.74 7.28±0.62
7.33±0.61 7.27±0.58
-0.06±0.44
-0.003±0.41 0.706
LF/HF ratio Laser
Sham
1.37±1.52 1.31±1.54
1.15±0.21 2.70±0.12
-0.04±0.12
-0.03±0.09 0.98
HRV : Heart rate variability, HRT : heart rate, SDNN : the standard deviation of normal-to-normal RR intervals, Ln(HF) : log-transformed high frequency power, Ln(LF): log-transformed low frequency power,
Ln(HF) : log-transformed high frequency power, Ln(TP) : log-transformed total power Δ: difference between pre-laser period and post-laser period
Laser was real laser group.
Sham was sham laser group.
Data are presented as the mean value±standard deviation Statistical significance was evaluated by independent T-test.
1),5),6)
.
(laser acupuncture)
( )
( ) .
, ( ), ( ), ( )
.
HeNe (633 nm) . HeNe
, ,
. (coherence length)
,
, InGaAlP
.
(Transcu-
taneous Blood Irradiation) .
7).
1). (therapeutic laser)
. HRV
. HRV (R-R interval)
,
. R-R
. 1960 fetal distress
, HRV
.
, ,
,
8),9)
.
5 R
8). HRV
(spectrum analysis) ,
(high frequency, HF), (low fre- quency, LF), (very low frequency, VLF)
(total power, TP) . Power
Spectrum HF 0.2-0.4 Hz
, LF 0.05-0.2 Hz
.
atropine HF
LF ,
. ,
SDNN RR 30-60 ms
, ,
LF/HF ratio
10). InGaAlP
HRV
. 62
, probe
. 5 , 5
HRV , 30
, 5 HRV .
independent t-test .
, Mean HRV 73.39±8.34 cycle/min, 75.58±
10.53 cycle/min 30 73.26
±9.12 cycle/min, 74.48±9.91 cycle/min , ,
. SDNN 44.26±15.55
ms, 42.45±12.36 ms 30
42.71±12.10 ms, 43.35±12.15 ms ,
, , . Ln(VLF)
6.54±0.85 logms2, 6.31±0.75 logms2
30 6.43±0.71 logms2, 6.25±0.79
logms2 , ,
. Ln(LF)
6.18±0.86 logms2, 6.09±0.67 logms2
30 6.21±0.79 logms2, 6.18±0.62
logms2 ,
. Ln(HF) 5.66±0.86
logms2, 5.95±0.85 logms2 30 5.52±0.96 logms2, 5.86±0.72 logms2
, ,
. Ln(TP) 7.38±0.74
logms2, 7.28±0.62 logms2 30 7.33±0.61 logms2, 7.27±0.58 logms2
. LF/HF ratio
1.37±1.52, 1.15±0.21 30
1.31±1.54, 2.70±0.12 , ,
.
Ln(VLF), Ln(HF), Ln(TP)
, Ln(LF) ,
Mean HRV, LF/HF, SDNN ,
. ,
,
,
.
(2004)4) TP(total power)
. Jan Tunér1)
, ,
.
.
30 1 ,
.
, ,
,
.
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, , , . Power
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. . 1997;21(5):928-35.
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. 2004;25(4):25-33.
5. . .
. 19991;8:7-10
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7. . : :
;1999, p79-81, 116.
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Standards of measurement, physiological inter-
pretation, and clinical use. Eur Heart J. 1996;
17(3):354-81.
9. , , , , , .
.
2002;23(12):1432-9.
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