CONCLUSION

We provide the evidence for both HSPGs and CSPGs as internalizing receptor of an anti-nucleic acid antibody, 3D8 scFv.

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３１ - 국문요약 -

항-핵산항체 (3D8 scFv)의 세포 내 유입에 관여하는 세포 표면 수용체 분자 규명

아주대학교 대학원 의생명과학과 김혜진

(지도교수: 권명희)

일부의 항-핵산항체는 다양한 종류의 세포 내로 유입 할 수 있다. 3D8 single chain

variable fragment (scFv)는 caveolae/lipid-raft 세포 내 이입 경로를 통해서 세포 내로 유입 할 수 있는 항-핵산항체이다. 그러나 3D8 scFv의 세포 내 유입에 관여하는 수용체는 아직 밝혀지지 않았다. 본 연구에서는 3D8 scFv의 세포 내 유입 수용체를 알아보고자, 공촛점현미경 분석법을 수행하여 다음을 관찰하였다.

HeLa세포에서 3D8 scFv는 여러 가지 리간드 (ligand)의 수용체로 알려져 있는 세포막 헤파란황산 단백당 (heparan sulfate proteoglycans: HSPGs)과 콘드로이틴황산 단백당 (chondroitin sulfate proteoglycans: CSPGs)에 결합하였다. 3D8 scFv와 가용성 헤파란황산 또는 콘드로이틴황산을 함께 세포에 처리하였을 때에 3D8 scFv과 세포 표면 헤파란황산 단백당과의 결합이 저해되었다. 3D8 scFv를 세포에 처리한 후 세포 안에서 세 가지 분자들의 공존 즉, 3D8 scFv-헤파란황산 단백당-카베올린

３２

1 (caveolin 1)의 공존 및 3D8 scFv-콘드로이틴 황산단백당-카베올린 1의 공존이 관찰되었다. 또한 3D8 scFv의 세포 내 유입이 세포막 헤파란황산 단백당과 콘드로이틴 황산단백당이 결여된 CHO 세포주에서 상당히 감소되었다. 이러한 결과들은 헤파란황산 단백당과 콘드로이틴황산 단백당이 3D8 scFv의 세포 내 유입 수용체임을 보여준다.

핵심어: 항-핵산항체, 3D8 scFv, 세포내 이입 수용체, 헤파란황산 단백당, 콘드로이틴황산 단백당