I screened 64 ethanol extracts of edible plants native to Korea were screened for their ability to increase cellular proliferation and differentiation using the osteoblastic cell lines C3H10T1/2 and MC3T3-E1 and selected Lycii Radicis Cortex (LRC), Lycium Chinense root bark, as a primary candidate because the treatment of LRC extract showed the greatest increase in cellular proliferation and differentiation of osteoblastic cells. The OVX mice administered LRC extract for either 8 or 16 weeks exhibited significantly increased bone mineral density compared to non-treated control OVX mice, and no toxic effects were observed.
This is the first report demonstrating the effect of LRC extract on bone formation.
Based on the in vitro and in vivo data, my results suggest that LRC extract may be a good candidate for the treatment of osteoporosis with few side effects.
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다음으로, 골다공증 모델 동물인 난소절제 마우스 실험을 통해 지골피
추출물의 in vivo 효과를 관찰하였다. 8 주간의 1 차 실험을 통해 지골피
추출물의 최적농도(150 mg/kg/day)를 결정한 후 16 주 동안 2 차 실험을 진행한 결과, 지골피 추출물이 골다공증이 유발된 마우스의 골밀도(bone mineral density) 개선에 유의한 효과가 있음을 증명하였다.
결론적으로, 본 연구에서는 지골피 추출물이 부작용이 적고 효과적인 골다공증 예방 또는 치료제로서의 활용 가능성이 있음을 처음으로 밝혔다.
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핵심어 : 천연물 추출물, 지골피, 골다공증, 조골세포, 난소절제 마우스, 골밀도