We demonstrated that preoperative BoTA therapy increases TRAM flap survival in a rat model by increasing HIF-1α/VEGF signaling-dependent angiogenesis.
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ABSTRACT(IN KOREAN)
백서의 횡복직근 피판 생존에 보툴리눔 독소 A가 미치는 영향
<지도교수 나 동 균>
연세대학교 대학원 의학과
박 태 환
본 연구에서는 백서의 횡복직근 피판 모델에 보툴리눔 독소 A 를
국소 적용하였을 때 혈관 확장 및 혈관 신생 관련 성장인자의 과발
현을 통해 횡복직근 피판 생존에 미치는 영향을 규명하고자 하였다.
24 마리의 백서를 보툴리눔 독소 국소 투여군과 생리식염수를 투여
할 대조군으로 나누었다. 먼저 복부의 칼돌기 직하방에서 5cm 길이의
직선 절개선을 가하고 봉합 시행 5 일 후에 실험군은 보툴리눔 독소
A 를 국소 투여하고 대조군에서는 같은 양의 생리 식염수를 투여하 였다. 주사 5 일 후에 횡복직근 피판을 거상하였다. 피판을 거상한 후 하방에 실리콘 시트를 적용한 후 피판을 원래 위치로 다시 위치시키
고 피판의 생존면적을 술 후 제1 일, 제 3 일, 제 5 일째 측정하였고 최
종 피판 생존면적은 술 후 5 일째 측정하였다. 헤마톡실린-에오신염
색을 통해 횡복직근 피판의 혈관경 면적의 변화 및 전체적인 조직학
적 변화의 양상, CD31 면역 화학 염색법을 통해 피판 내 미세혈관농
도를 각각 술 후 5 일째 평가하였다. 그리고 같은 시기에 혈관신생에
관여하는 인자인 CD34, HIF-1α, VEGF 의 유전자 및 단백질을 정량적
Publication list
1. Park TH, Rah DK, Chong Y, Kim JK. The effects of botulinum toxin A on survival of rat TRAM flap with vertical midline scar. Ann Plast Surg 2015;74:100-6.
2. Park TH, Lee SH, Park YJ, Lee YS, Rah DK, Kim SY. Presurgical Botulinum Toxin A Treatment Increases Angiogenesis by Hypoxia-Inducible Factor-1α/Vascular Endothelial Growth Factor and Subsequent Superiorly Based Transverse Rectus Abdominis Myocutaneous Flap Survival in a Rat Model. Ann Plast Surg 2016;76:723-8.