In this study, we isolated and identified a new type of MSCs from mastoid bone marrow and adipose tissue obtained during mastoidectomies. Both hMBMCs and hEASCs possessed the general features of MSCs, including morphology, stemness, and multiple differentiation including adipogenesis, chondrogenesis, osteogenesis, neurogenesis, and epithelial differentiation under specific stimulation. Also, we have shown for the first time that hEASCs combined with porous PCL scaffolds significantly facilitated mastoid bulla bone formation. Thus, this combination of hEASCs with PCL scaffolds represents a promising approach for the anatomical and functional reconstruction of postoperative temporal bone defects following mastoidectomy.
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분석하였다. CT를 시행한 결과, 귀 지방유래 세포 + PCL 지지체군에서 상당한 골 분화가 관찰되었으며, H&E와 면역염색 결과에서도 유양골 결손 기저부로부터 골형성이 시작되는 것을 확인하였다. 이에 골 분화촉진 성장인자군이 첨가되었을 때, 유양골 형성의 상승효과가 관찰되었다. 그러나 이러한 효과가 두개골 결손모델에서는 관찰되지 않았다. 본 연구에서는 귀 지방유래 세포와 3 차원 다공성 지지체를 이식하여 유양골 형성의 유의미한 효과를 보았다. 향후 유양돌기 절제술시 발생하는 부작용에 자가조직과 지지체를 이용한 본 기술이 유용하게 적용될 것으로 기대된다.
핵심어: 골형성, 유양돌기절제술, 유양돌기, 지방조직, 줄기세포