D. 하인두암 세포주에서 Western blotting 을 이용하여 HGF 의 농도에 따른
Ⅴ. 결론
참고문헌
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-ABSTRACT-
Expression of E-cadherin and HGF in Hypopharyngeal Cancer
Jang Woo Lee
Department of Medical Sciences The Graduate School, Ajou University
(Supervised by Assistant Professor Chul-Ho Kim)
Background and Objectives: Head and neck squamous cell carcinoma (HNSCC) results from a multistep carcinogenesis process in which increasing degrees of mucosal changes and cellular atypia occur over large areas of epithelium.
Hypopharyngeal squamous cell carcinoma(SCC) is well known as a aggressive cancer which has relatively poor prognosis. E-cadherin is a large family of Calcium dependent intercelluar Adhesion Molecule(CAM) whose functions are essential for the induction and maintenance of intercellular cohesion and hepatocyte growth factor(HGF), a potent stimulator of hepatocyte growth, stimulates motility, invasiveness, proliferation, and morphogenesis of epithelium, and also may be involved in tumor invasion. So we examined the role of the E-cadherin/HGF on invasion and metastasis of hypopharyngeal squamous cell carcinoma(SCC).
Materials and Method: We performed immunohistochemical stains on 40 tissues of hypopharyngeal cancer patients who were admitted to Ajou University Hospital &
Severance Hospital between Mar. 1994 and Dec. 2003 with E-cadherin antibody. We classified the stains according to the intensity and location of E-cadherin expression, and analysis the correlation between the intensity or location of E-cadherin
expression and the many clinicopathologic factors. To find the effect of HGF for the E-cadherin expression in the progression of hypopharyngeal cancer, RT-PCR and Western blot in hypopharyngeal cell line(FaDu) were performed according to the concentration of HGF(0, 10, 30 ng/ml). Hypopharyngeal cancer cell line
Results: All tissues were positive for the immunohistochemical staining of E-cadherin. According to the intensity +, ++ and +++ group were 25%, 55% and 20%
respectively and according to the location membranous and non-membranous group were 50% respectively. Although the intensity of E-cadherin was not correlate with any clinical factor, the location of E-cadherin was siginificantly correlated with lymph node metastasis(p<0.05). Analyze for RT-PCR and Western Blot of E-cadherin expression in FaDu cells revealed the decreased expression of E-E-cadherin in proportion to the concentration of HGF.
Conclusion: These results suggest that non-membranous expression of E-cadherin in hypopharyngeal cancer was significantly correlated with lymph node metastasis and that HGF may plays an important role in progression of hypopharyngeal cancer through significantly reducing the expression of E-cadherin.
Key words: E-cadherin, HGF(Hepatocyte Growth Factor), Hypopharyngeal cancer, Invasion, Metastasis