JFS

(1)

Supplementary figure 1. Symptoms on Ligusticum chuanxiong by infection of Neocosmospora solani. A: L. chuanxiong showing root and collar rot. B: Close-up view of L.

chuanxiong showing the symptom of collar rot.

(2)

Supplementary figure 2. Maximum-Likelihood tree of Neocosmospora species based on the concatenated data set including the internal transcribed spacer regions (ITS1, ITS2) and 5.8S rDNA gene regions and part of the translation elongation factor-1 alpha (tef-1α) regions. Only ML bootstrap values ≥ 50% are indicated on the nodes. Geejayessia atrofusca (NRRL 22316) and G. cicatricum (CBS 125552) were used as outgroup. T, ET, LT, and NT indicate type, epitype, lectotype, and neotype strains, respectively.

(3)

Supplementary table 1. Cultures and DNA accession numbers used for phylogenetic analysis in this study

1

Species Name Culture^* Substrate GenBank accession number

ITS tef-1α

Geejayessia atrofusca NRRL 22316 Staphylea trifolia AF178423 AF178361

G. cicatricum CBS 125552 Dead twig HQ728145 HM626644

Neocosmospora ampla CBS 202.32^T Coffea sp. LR583701 LR583594

N. batatas CBS 144398^T Ipomoea batatas AF178408 AF178344

N. bostrycoides CBS 144.25^NT Soil LR583704 LR583597

N. elegans CBS 144396^ET Trunk of Xanthoxylum piperitum AF178401 AF178336

N. falciformis CBS 475.67^T Human mycetoma MG189935 LT906669

N. haematococca CBS 119600^ET Dying tree KM231797 DQ247510

N. henyangensis HMAS 254518^T Twigs of unknown host KY829446 KY829448

N. illudens NRRL 22090 Beilschmiedia tawa AF178393 AF178326

N. kurunegalenis CBS 119599^T Tree JF433036 DQ247511

N. lichenicola CBS 279.59^T Soil LR583726 LR583616

N. liriodendri CBS 117481^T Liriodendron tulipifera AF178404 AF178340

N. longissima CBS 126407^T Tree bark LR583731 LR583621

N. macrospora CBS 142424^T Citrus sinensis LT746266 LT746218

N. martii BPI 452385^LT Solanum tuberosum LR583735 LR583625

N. noneumartii CBS 115658^T Solanum tuberosum LR583745 LR583630

N. oligoseptata CBS 143241^T Euwallacea validus on Ailanthus altissima KC691566 KC691538

N. paraeumartii CBS 487.76^T Solanum tuberosum LR583747 DQ247549

N. pseudensiformis CBS 125729^T Dead tree KC691584 DQ247512

N. pseudoradicicola CBS 145472^T Diseased cocoa pods JF740899 JF740757

(4)

N. rectiphora HMAS 254519^T Twigs of unknown host KY829447 KY829449

N. solani CBS 101018^T Raspberry LR583770 LR583651

N. solani CDH 2020-1 Ligusticum chuanxiong MT269073 MT263069

N. stercicola CBS 142480^T Branch of Hibiscus sp. LR583778 KY556525

N. tonkinensis CBS 115.40^T Musa sapientum MG189941 LT906672

* T, ET, LT, and NT represent type, epitype, lectotype, and neotype strains, respectively.

2