First Record of Nipponopsyche fuscescens Yazaki, 1926 (Lepidoptera, Psychidae) from Korea with a Redescription of External Morphology

The family Psychidae is consisting of 241 genera and 1,350 species (Sobczyk, 2011; van Nieukerken et al., 2011).

Among then, the subfamily Oiketicinae was established by Herrich-Schäffer in 1855 based on the type genus, Oiketicus Guilding, 1827. The Oiketicinae species have a relatively large-sized, consisting more than 388 described species under 4 tribe worldwide (Sobczyk, 2011).

The genus Nipponopsyche belongs to Oiketicinae, based on the type species Nipponopsyche fuscescens Yazaki, 1926.

In the world, the genus Nipponopsyche has been known only

one species, which was distributed in Japan (Sauter and Hättenschwiler, 1991; Sobczyk, 2011; Saigusa and Sugimoto, 2013). Yazaki (1926) diagnosed the genus Nipponopsyche with the combination of the following adult characters:

antennae bipectinate and scape very large, forewing very broad with nine veins arising from discal cell. As other species of Psychidae, larvae of the genus tend to build their cases putting together feed-grasses. The genus Nipponopsyche mainly live in low-elevation grassland, wasteland or wetland in their larval stage. However, some habitat has been reduced by the use of power brush cutter recently (Saigusa and Sugimoto, 2013).

In the present study, N. fuscescens is newly recognized from South Korea for the frist time. Also, all the available information is presented, including the collection locations,

Original article KOREAN JOURNAL OF APPLIED ENTOMOLOGY

한국응용곤충학회지 ⓒ The Korean Society of Applied Entomology

Korean J. Appl. Entomol. 58(1): 1-8 (2019) pISSN 1225-0171, eISSN 2287-545X

DOI: https://doi.org/10.5656/KSAE.2018.10.0.042

First Record of Nipponopsyche fuscescens Yazaki, 1926 (Lepidoptera, Psychidae) from Korea with a Redescription of External Morphology

Seung Jin Roh, Da-Som Kim

, Bo-Sun Park, Subin Choi and Bong-Kyu Byun

*

Division of Forest Biodiversity, Korea National Arboretum, Pocheon 11186, Korea

1

Department of Biological Science and Biotechnology, Hannam University, Daejeon 34054, Korea

2

Research and Promotion Division, National Science Museum, Daejeon 34143, Korea

한국미기록종 잔디주머니나방(나비목: 주머니나방과) 보고 및 형태특징 재기재

노승진ㆍ김다솜

ㆍ박보선ㆍ최수빈ㆍ변봉규

*

국립수목원 산림생물조사과, ¹한남대학교 생명시스템과학과, ²국립중앙과학관 자연사연구실

ABSTRACT: The genus Nipponopsyche Yazaki is reported from Korea with the species, N. fuscescens Yazaki for the first time. Adult including genitalia, larva, and pupa of the species are redescribed, and DNA barcode for precise identification of the species is also provided.

Key words: Oiketicinae, Bagworms, Parthenogensis, DNA barcode

초 록 : 본 연구를 통해 잔디주머니나방속이 국내에서 처음 발견되어 보고한다. 잔디주머니나방속내 잔디주머니나방에 대한 성충, 유충, 번데기 등 형태학적 특징을 재기재하였다. 또한 신속한 종 동정을 위한 DNA바코드 정보를 제공하였다.

검색어: 검정주머니나방아과, 도롱이벌레, 처녀생식, DNA바코드

*Corresponding author: [email protected]

Received September 5 2018; Revised October 31 2018

Accepted January 3 2019

microhabitats, and illustrations of larva, pupa, adults and its genitalia with SEM. DNA barcode is also provided for precise identification of the species.

Materials and Methods

The materials examined in this study are based on the Entomological Collection of the Korea National Arboretum, Pocheon, Korea (KNAE). Specimens were dissected and examined after mounting on slide glass; male genitalia and wing scales in 80% glycerol solution and wing venation on dried condition. Photographs of adults and genitalia were taken using a DFC 495 digital camera attached to Leica M205A stereomicroscope (Leica, Wetzlar, Germany). Also, drawing of the wing venation was taken using a Adobe Illustrator CS5 and Adobe Photoshop CS6 (Adobe systems, San Jose, USA). To examine some morphological character of larvae, scanning electron microscopy (SEM) was used. For the dried specimen, the larvae dehydrated on 50-99% ETOH in order. And then, critical poing dryer (EMITECH K850, Ashford, Kent, UK) was used for complete dehydration. Each specimens were gold coated using Ion Sputter E-1010 (HITACHI, Tokyo, Japan) and examined with S-3400N (HITACHI, Tokyo, Japan) electron scanning microscope.

Terminology and morphological characters follows Kris- tensen (2003), Sugimoto and Saigusa (2001), Kozhanchikov (1956).

Genomic DNA was extracted from the legs of dried specimen for males and thorax parts of immersion specimens for females and larva, preserved in 100% alcohol using a Genomic Cell/Tissue Spin Mini Kit (Qiagen, Inc, Hilden, Germany), according to the manufacturer’s protocol. A total of three specimens were sequenced for 658 bp fragment of the mitochondrial cytochrome c oxidase I (COI) gene, and the DNA barcode was amplified using the primer LepF1, LepR1 (Hebert et al., 2004). PCR conditions for amplification followed the manufacturer’s protocol (Platinum Taq, Invitrogen, Carlsbad City, CA, USA). The amplicons were purified using the QIAquick® PCR purification kit (QIAGEN, Inc, Hilden, Germany) and directly sequenced at Macrogen (Seoul, Korea).

Contigs were assembled using CodonCode aligner version 2.0.6 (CodonCode Co., Centerville City, MA, USA). Successful

sequences were uploaded to BOLD systems (BOLD BIN no.

BOLD:ADJ9301).

Taxonomic accounts

Genus Nipponopsyche Yazaki, 1926 잔디주머니나방속(신칭) Nipponopsyche Yazaki, 1926: 173, 174.

Type species: Nipponopsyche fuscescens Yazaki, 1926: 174 by monotypy.

Nipponopsyche fuscescens Yazaki, 1926 잔디주머니나방(신칭) Nipponopsyche fuscescens Yazaki, 1926: 174. Type locality:

Japan.

Adult. Male (Fig. 1). Wingspan 17.3-21.2 mm. Coloration and vestiture: sclerites on head and thorax dark-brown. Head (Fig. 1D and E) clothed with slightly long light brown hairs dorsally and anteriorly. Compound eyes blackish. Thoracic notum densely clothed with long brown hairs downwardly.

Forewings: ground color light brown; generally covered brown scales. Hindwing covered with brown scales, basal part slightly short brown hairs. Legs (Fig. 1F) red-brown on coxae, femora and tibiae; coxae and femora covered with brown long hairs; tarsi covered with short light brown hairs; claws reddish brown. Structure: head including eyes nearly 2/5 as wide as thorax in dorsal aspect; mouthparts mostly reduced. Ocelli absent. Antenna (Fig. 1C): basal flagellomeres 29 segmented, pectinate, slightly longer than 1/2 forewing, length of antenna nearly 3.4-3.7 mm, flagellomeres covered with dark-brown scales. Forewing (Fig. 1B) short and wide, nearly triangular, costa straightly, then gently arched beyond 4/5, apex very obtuse, posterior margin straight downwardly. Scales (Fig. 1G) very slender, truncate apical margin usually produced into 1-3 weak rounded laciniation. 9 veins arising from discoidal cell;

discoidal cell longer than 0.67 times as long as wing, distal

corner of anterior part rectangular; Sc reaching to 3/5 costa; R

and R

originated at anterior part of discoidal cell; R

and R

stalked at apical corner of anterior part of the discoidal cell; R

reaching to the near apex; R

arising to 2/5 R

; M

absent; M

and M

originated at apical corner of posterior part of discoidal

cell; CuA

and CuA

close to distal corner of posterior part of

discoidal cell; CuP weak; A1+A2 straight at hind margin.

Hindwing (Fig. 2) fan shape. Scales (Fig. 1H) slender with arrowhead shape. Costa slowly curved, discoidal cell 3/5 length of hindwing with asymmetrical, gradually dilating

apically; posterior part of discoidal cell longer than anterior part; Sc+R

reaching to 4/5 of costal margin; Rs ending at apex; M

absent; M

and M

originated at distal corner of

Fig. 1. Adult, male: A, male; B, wing venation; C, antenna; D, head, front; E, ditto, lateral aspect; F, legs; G, forewing scales; H, hindwing scales.

Fig. 2. Adult, female: A, lateral aspect; B, ventral aspect; C, dorsal aspect; D, head and thorax, lateral aspect; E, head, front; F, 8 to 10

segments (genitalia); G, legs.

posterior part of discoidal cell and parallel; CuA

arising to 1/3 M

; CuP straight to termen.

Male genitalia (Fig. 3). Genitalia coverd with long 8

abdominal segment. In lateral aspect, dorsum nearly curved;

saccus very long; ampulla club shape with setae sparsely;

harpe hooked apically. In dorso-ventral aspect, uncus very weak concave, tegumen broad, valva short and apex rounded, vinculum wide and rapidly arched to saccus, saccus slender and elongated downwardly, phallus shorter than genitalia.

Female (Fig. 2). Length 12.5-13.9 mm, vermiform, cylindrical, prothorax, mesothorax and metathorax thin, almost same thickness from the 3

to 6

abdominal segment, 8+10

abdominal segments with short papillae anales. Coloration:

head brown with matt vertex. Meso and metanotum brown.

Membranous areas of abdomen yellowish. Structure: head (Fig. 2D and E) reduced in size, about 0.05 times as long as body length; mouth part considerably degenerated, antennae very short, eye-spots blackish. Thorax very small and 0.07 times as long as body length, dorsal margin slightly curved and covered with few scales, prothorax narrower than meso and metathorax. Legs (Fig. 2G) almost degenerated with very short. Abdomen well developed and 0.82 times as long as body length, first abdominal segment weak sclerotized, second to

6

abdominal segments almost membranous, 7

abdominal segment approximately 1.2 times as long as the 6

segment, 7

segment (corethrogyne or anal hair tuft) covered with few hairs, eighth abdominal segment very short, 9

and 10

abdominal segments present papillae anales (ovipositor), papillae anales very short.

Larva (Fig. 4). Length 14-16.3 mm. Coloration and vestiture: head present thick black lines, epicranial suture black, frons pale yellow, clypeus and labrum dark brown.

Dorsal aspect of thorax pale yellow with thick black lines.

Abdomen brown, black lines present in dorsal aspect;

membranous parts and sclerites pale yellow. Thoracic legs pale yellow with black some line. Abdominal and anal proelg pale yellow. Structure: head slightly large; epicranial notch concave, epicranial suture slightly thick, adfrontal area very thick and slightly curved downwardly; antenna short; frons triangular, C1 setae coiled; clypeus protuberant weakly;

labrum slightly narrow and concave at ventral aspect;

mandible broad. Thoracic legs slightly thick with many setae, pretarsus hooked; prothoracic shield well sclerotized.

Abdominal prolegs short. Anal shield weak sclerotized, anal proleg very short, abdominal proleg crochets U-shaped with short setae; spiracles oval shaped.

Fig. 3. Male genitalia: A, dorso-ventral aspect; B, dorsum, dorsal aspect; C, 8

abdominal segment; D, lateral aspect.

Pupae (Fig. 5). Male (Fig. 5A-C): length 10.7-11.5 mm.

reddish brown on head, thorax and abdomen, brown on legs.

Body cylindrical, present setae sparsely. Swollen at vertex;

pronotum slightly arched; maxillae considerably thick;

maxillary palps triangular shape; antennae quite wide with

transverse line regularly; I and II thoracic legs slightly slender

Fig. 4. Larva: A, head, front; B, thorax and abdomen, lateral aspect (T: thorax; A: abdomen); C, head and thoracic legs, dorso-ventral aspect

(A: anterior setae; C: clypeal setae; F:frontal setae); D, labrum and mandible (L: lateral setae; M: mandibular setae); E, 3 to 6

abdominal

segments; F, 6 to 10

abdominal segments; G, abdominal proleg; H, ditto, lateral aspect; I, spiracle; J, ditto, close-up; K, seta; L, setae,

close-up.

and straight downwardly. Forewing moderately wide, comprising 2/5 of pupa; hindwing narrowed to posterior margin of abdomen. posterior margin of mesonotum wide and U-shape; metanotum narrow and slighlty convex. second to 6

abdominal segments almost same thickness, 7

to 9

narrowed thickness; 9

abdominal segment slightly rounded. Anal hooked.

Female (Fig. 5D-F). Length 14-15.4 mm. Body reddish brown, covered with some scales, nearly same width and thickness as 4

abdominal segment, evenly narrowed to tip.

Head very small, considerably degenerated. Thorax narrowly

simple form, three thoracic segments of nearly the same length with less than 1/10 of pupa. Abdominal segments slightly tapered in basally. Anal slightly blunt.

Larval case (Fig. 7). In the observation, this Larvae build their cases by putting together lawn grasses. Also, they are live around the grave.

Material examined. 4 ♂, 33♀ (emerged individuals), 4 larvae, Chuja Is., Jeju Is., lat.33.96399722, long.126.2879, 30.v.2018 (S.J. Roh, D.S. Kim, B.S. Park and S.B. Choi), genitalia mounted on 80% glycerol solution, gen. no.

KNAESJ00044, scales of wing mounted on 80% glycerol

Fig. 5. Pupae: A, male, lateral aspect; B, ditto, ventral aspect (mx.p.: maxillary palps; mx.: maxillae); C, ditto, dorsal aspect; D, female, lateral

aspect (T: thorax; A: abdomen); E, ditto, ventral aspect; F, ditto, dorsal aspect.

Fig. 6. Parthenogenetic individual: A, pupal case of female; B, first instar larva, dead condition; C, first instar larvae, hatch from eggs; D, larva and larval case.

Fig. 7. Feeding habits of N. fuscescens: A, feeding on lawn grass (lat. 33.96399722, long. 126.2879); B, ditto: C, larvae of N. fuscescens; D,

ditto, close-up.

solution, sclaes of wing no. KNAESSJ10, wing venation no.

KNAEVS18-coll. KNAE.

Distribution. Korea (new record), Japan.

DNA barcode. DNA barcode sequence were generated (BOLD Process ID. NFNK001-18, NFNK002-18, NFNK003-18).

Multiple alignments using the BLAST tool in the BOLD systems database showed the following species as nearest neighbor: Nipponopsyche fuscescens (locality of reference data from Japan) 99.85%.

Remarks. Parthenogenesis is known at least four subfamilies and five genera in the Psychidae (Sobczyk 2011; Elzinga et al., 2013). In this study, we found that N. fuscescens occurrs parthenogenetic form (Fig. 6). This Larvae build their cases by putting together lawn grasses. Also, they are live around the grave (Fig. 7). Adults emerge from late June to late July in breeding condition. Out of 37 larvae, 33 females and 4 males were emerged in the present study.

Acknowledgements

We thank to Miss YM Shin (Entomological Collection of the Korea National Arboretum, Pocheon, Korea) for their assistance in breeding samples. Our thanks are also due to Mr.

GS Bae, for taking a SEM images. This study was carried out for 23

joint academic investigation by Korean Biodiversity Consortium with KBIF. This work was funded by National Research Foundation (NRF-2018X1A3A1070549) and Korea National Arboretum (Project No. KNA 1-1-20, 16-1).

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