The Effects of Gammaekdaejo-tang (Ganmaidazao-tang) on Rats Subjected to Stress by Immobilization

(1)

서 론

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2007 8 16 2007 8 17

2007 9 3

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(Tel : 054-770-2361, Fax : 054-770-2281, E-mail : kjh@dongguk.ac.kr)

甘麥大棗湯白鼠影響

백현, 김장현

원 저

The Effects of Gammaekdaejo-tang (Ganmaidazao-tang) on Rats Subjected to Stress by Immobilization

Hyun Baek, Jang-hyun Kim

Dept. of Pediatrics, College of Oriental Medicine, Dongguk University

Objective : This study was conducted in order to find the effects of Gammaekdaejo-tang (Ganmaidazao-tang, GDT) by subjecting rats to immobilization stress, thereby inducing depression, anxiety and acquisition-retention defects.

Method : Rats treated with normal saline, GDT 200mg/kg and GDT 400mg/kg were subjected to stress by immobilization. Afterwards, behavior changes were observed by elevated plus maze test, acquisition test and retention test in the Morris water maze. The results were obtained by immunohistochemically measuring stress hormone (corticosteroid) levels in the blood.

Results and Conclusions :

1. The open arm test in the elevated plus maze showed that compared with the normal group, the time spent decreased in the control group and increased in the GDT 400mg/kg group.

2. The locomotor activity test in the elevated plus maze revealed that the control group showed significant activity decrease compared with the normal group but significant increase in the GDT 400mg/kg group.

3. The acquisition test in the Morris water maze showed that the acquisitive ability of the control group significantly deteriorated on the 3rd and 4th day compared with the normal group, but improved significantly in the GDT 200mg/kg and GDT 400mg/kg groups.

4. The retention test on the 7th day in the Morris water maze revealed that the retentive ability of the control group significantly deteriorated compared with the normal group, but the retentive ability of the GDT 400mg/kg group significantly improved.

5. The blood levels of corticosteroid in the control group increased significantly compared with the normal group but the levels of corticosterone in the blood of the GDT 400mg/kg group significantly decreased.

Key Words : gammaekdaejo-tang (ganmaidazao-tang, GDT), immobilization stress, elevated plus maze (EPM) test, morris water maze (MWM) test, corticosteroid

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corticosterone

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재료 및 방법

1. 材料 1)

8 200-250g Sprague-Dawley

(( ) , ) .

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.

9 12 , 9

12 .

21°C, 50±5% .

Scientific name amount(g)

Tritici Immatri Semen ( ) 28.0

Glycyrrhizae Radix(roasted) ( ( )) 6.0

Jujubae Fructus ( ) 5.0

Total 39.0

(3)

2)

. (Gamma-

ekdaejo-tang, GDT) ⁷⁾

1

Table 1 .

2. 方法 1)

10 6

110 3 .

65 rotary

vacuum evaporator 70%

4-5 .

18.0% , .

2) 4 .

(Normal group; N=11)

(Control group; N=10) 200 /

(GDT ; N=11) 400 /

(GDT ; N=10)

10 200 /

, 400 / 30 . 3)

(immobilization stress)

10 2 3

. ,

,

10). disposable rodent rest- raint cone(Havard 52-9586, Havard instrument, USA)

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4) Elevated plus maze(EPM) ^11,12) (1)

EPM

(closed arm),

(open arm). 60cm

.

, 5

,

. 30

EPM .

. (2)

EPM ,

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5) Morris water maze(MWM) ¹³⁾ (1)

3 1

MWM .

180cm, 50cm

22±2 30cm .

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12cm ,

1.5cm . 4

(NE),

(NW), (SE), (SW)

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4 7 ,

, (acquisition test)

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4 7

7

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. 7 (retention test)

. 6) corticosterone

pentobarbital 0.1 / ,

-70 , Cort kit

corticosterone .

3. 통계처리

± ,

window SPSS 8.0

repeated ANOVA , one-

way ANOVA , Tukey

, P 0.05 .

결 과 1. EPM test

1)

Group Mean±S.E.M.(%)

Normal Control GDT GDT

100.0±0.0 74.5±3.5**

82.1±3.9 93.1±4.6+

Normal : non-stressed group, Control : treated with saline and stressed group, GDT : treated with GDT 200mg/kg and stressed group, GDT : treated with GDT 400mg/kg and stressed group

** Significantly different from the normal group(p<0.01) + Significantly different from the control group(p<0.05)

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74.5±3.5%

(p<0.01) , GDT

93.1±4.6%

(p<0.05) (Fig. 1, Table 2).

2)

8.3±2.1

14.5±2.3 (p<0.05)

, GDT

13.5±2.4 (p<0.05)

(Fig. 2, Table 3).

2. MWM 검사 1)

MWM 3

110.5±10.4 sec 78.6±18.3 sec

(p<0.05) GDT

86.0±10.2 sec (p<0.05)

.

120 100 80

60 40

Normal 0

20

**

+

Control GDT I GDT II

Time in open arm(%)

Group Mean±S.E.M.(number)

14.5±2.3 8.3±2.1*

11.3±3.2 13.5±2.4+

* Significantly different from the normal group(p<0.05) + Significantly different from the control group(p<0.05)

(6)

4

92.1±10.4 sec 49.4±11.5 sec

(p<0.01) , GDT

, 61.4±9.3 sec, 60.5±9.7 sec ( p<0.05)

(Fig. 3, Table 4).

2)

15.6±4.3%

30.7±5.6% (p<0.01)

, GDT 28.4±3.6%

(p<0.05) (Fig. 4, Table 5).

3. 혈청 corticosterone 측정

corticosterone

16 14 12 10

Normal 0

20

*

+

8 6 4 2

Number of entered arm

Morris water maze acquisition test(Mean±SEM)(sec)

Group Day 1 Day 2 Day 3 Day 4 Day 5 Day 6

Normal 141.0±24.3 100.4±21.5 78.6±18.3 49.4±11.5 43.1±8.1 41.3±5.6 Control 143.5±21.6 111.5±11.5 110.5±10.4* 92.1±10.4** 56.2±9.2 48.5±8.7

GDT 142.7±17.9 118.5±14.5 98.0±11.3 61.4±9.3+ 51.3±8.6 43.1±7.8

GDT 135.6±21.6 119.5±15.2 86.0±10.2+ 60.5±9.7+ 52.3±10.3 35.0±9.3 Normal : non-stressed group, Control : treated with saline and stressed group, GDT : treated with GDT 200mg/kg and stressed group, GDT : treated with GDT 400mg/kg and stressed group

* Significantly different from the normal group(p<0.05)

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124.5±8.5 / 61.8±4.5 / (p<0.01)

, GDT 103.2±7.8 /

(p<0.05) ,

GDT 101.5±7.2 /

(p<0.01) (Fig. 5, Table 6).

고 찰

(depressive neurosis)

14).

,

180.0 160.0 140.0 120.0 100.0 80.0 60.0 40.0 20.0

0.0

1 2 3 4 5 6

Time (day)

Latency to platform(sec)

Normal Control GDT I GDT II

Group Mean±S.E.M.(%)

30.7±5.6 15.6±4.3**

24.0±5.2 28.4±3.6+

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35 30 25 20

Normal 0

**

+

15 10 5

Time in quadrant(%)

Group Mean±S.E.M.( / )

61.8±4.5 124.5±8.5**

103.2±7.8+

101.5±7.2+

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Blood level (ug/dL)

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holine receptor [H3]norepinephrine

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, EPM test, MWM test

,

corticosterone .

Handley Mithani EPM

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EPM .

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corticosterone .

,

corticosterone

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. Glucocorticoid

, corticosterone

(12)

corticosterone

.

, corticosterone

,

.

결 론

elevated plus maze test, Morris water maze test

,

corticosterone . 1.

74.5±3.5%

, 400 /

93.1±4.6%

. 2.

8.3±2.1 14.5±2.3

,

400 / 13.5±2.4

. 3.

3

400 / 86.0±10.2 sec

. 4

92.1±10.4 sec 49.4±11.5 sec

, 200 /

400 / 61.4±9.3 sec,

60.5±9.7 sec .

4. 7

15.6±4.3% 30.7±5.6%

, 400 /

28.4±3.6%

.

5. ·

corticosterone ,

124.5±8.5 / 61.8±4.5 /

, 200 /

103.2±7.8 /

, 400 /

101.5±7.2 /

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The Effects of Gammaekdaejo-tang (Ganmaidazao-tang) on Rats Subjected to Stress by Immobilization

甘麥大棗湯 白鼠 影響

The Effects of Gammaekdaejo-tang (Ganmaidazao-tang) on Rats Subjected to Stress by Immobilization

甘麥大棗湯白鼠影響