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Evaluation of Sperm Sex-Sorting Method using Flow Cytometry in Hanwoo (Korean Native Cattle)

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Academic year: 2021

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Table  1.  Composition  of  HEPES  sheath  fluid  buffer  for  sex-  sorting  of  bull  sperm  using  flow  cytometry
Fig.  1.  R3  and  R4  peaks  of  the  histogram  matched  the  gated  region  of  the  dot  plot  created  by  BD  FACSDiva  software
Fig.  3.  Embryo  sexing  after  PCR  with  the  S4BF/R  primer  set  to  determine  the  efficiency  of  the  sex-sorting  method  based  on  FSC  and  DNA  content
Table  2.  The  proportions  of  male  and  female  embryos  rates  at  48  h  after  IVF  with  sex-sorted  sperm  in  Hanwoo  cattle

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