제18강
<강의 핵심 내용>
* Flybase 웹사이트를 통해 관심 있는 유전자에 대해 조사한다.
www.flybase.org
*유전자: CG17109
*초파리의 생활사
CG6726 family
CG Num. a.a length a.a Seq similarity Highest expression Tissue CG6726 401
85.75%
Malpighian tubule CG17110 402CG17109 400 CG6733 401 CG6738 401
Malpighian tubule
Malpighian tubules (MT) are osmoregulatory organs that maintain the ionic balance and
remove toxic substances from the body. Additionally they act as autonomous immune sensing organs, which secrete antimicrobial peptides in response to invading microbial pathogens.
Drosophila AMP Gram (+)
mtk, def Gram (-)
att, dip, cec, drc Fungi
mtk, def, drm
MT – The renal tube
PC : H+를 능동수송으로 lumen으로 보냄.
H+농도기울기를 이용, K+역수송.
K+ 혈림프로부터, 세포를통해 lumen으로 보냄.
SC : PC가 생성한 K+ 농도기울기 때문에 Cl-채널로 Cl-을 lumen 으로.
생성된 삼투압으로 물을 아쿠아포린을 통해 이동시킴.
SC (stellate cell) PC (principal cell)
IMD pathway
• Gram (-)
• PAMP* :
LPS, DAP-PGN, TCT
• PRR** :
PGRP-LC, PGRP-Lx• txn factor :
Relish• AMP :
att(attacin) dip(diptericin) cec(cecropin) drc(drosocin)*PAMP : pathogen-associated microbial patterns
**PRR : pattern recognition recepter
Toll pathway
• Gram (+), fungi
• PAMP :
Gram(+) : Lys-PGN fungi : β-glucan
• PRR :
PGRP-SD, PGRP-SA• txn factor :
DIF• AMP :
mtk(metchnikowin) def(defensin)
drm(drosomycin)
* In situ hybridization
Polytene chromosome
<ISH website 조사>
BDGP: insitu.fruitfly.org
* 유전자: CG17109
* Check list for CG17109
5-1. cytological location
5-2. molecular function and biological process 5-3. how many transcripts and CDs
5-4. the most important domain
5-5. expression profile. ISH vs microarray 5-6. how many alleles
5-7. major phenotypes 5-8. how many references
